Animal-Free HDGF Protein, Mouse (His)
Based on 1 Customer Validation
HDGF Protein, a transcriptional repressor, promotes fibroblast mitogenic activity. Existing as a monomer or domain-swapped homodimer, it interacts with nuclear proteins NCL and YBX1/YB1. Animal-Free HDGF Protein, Mouse (His) is the recombinant mouse-derived animal-FreeHDGF protein, expressed by E. coli , with C-His labeled tag. This product is for cell culture use only.
- Species: Mouse
- Source: E. coli
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Biological Activity
Description
HDGF Protein, a transcriptional repressor, promotes fibroblast mitogenic activity. Existing as a monomer or domain-swapped homodimer, it interacts with nuclear proteins NCL and YBX1/YB1. Animal-Free HDGF Protein, Mouse (His) is the recombinant mouse-derived animal-FreeHDGF protein, expressed by E. coli , with C-His labeled tag. This product is for cell culture use only.
Background
HDGF Protein functions as a transcriptional repressor and exhibits mitogenic activity for fibroblasts. This heparin-binding protein can exist as a monomer and as a domain-swapped homodimer. Additionally, it interacts with nuclear proteins NCL and YBX1/YB1.
Technical Parameters
-
Species Mouse
-
Source E. coli
-
Tag C-6*His
-
Accession
P51859 (M1-L237)
-
Molecular Construction
-
N-term
-
HDGF (M1-L237)
Accession # P51859 -
6*His
-
C-term
-
-
Protein Length
Full Length
-
Synonyms
HDGF; Hepatoma-Derived Growth Factor; Heparin Binding Growth Factor; Hepatoma-Derived Growth Factor (High-Mobility Group Protein 1-Like); HMG1L2; High-Mobility Group Protein 1-Like; Epididymis Secretory Sperm Binding Protein; Hepatoma-Derived Growth Facto
-
AA Sequence
MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCAAEPEVEPEAHEGDGDKKGSAEGSSDEEGKLVIDEPAKEKNEKGTLKRRAGDVLEDSPKRPKESGDHEEEDKEIAALEGERPLPVEVEKNSTPSEPDSGQGPPAEEEEGEEEAAKEEAEAQGVRDHESL
-
Predicted Molecular Mass
27.1 kDa
-
Molecular Weight
Approximately 35-48 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a solution containing 1X PBS, pH 7.4, trehalose.
<0.1 EU per 1 μg of the protein by the LAL method.
It is recommended to reconstitute to a concentration of 100-200 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (236 KB)
-
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)