1. Recombinant Proteins
  2. Cytokines and Growth Factors Animal-free Recombinant Proteins
  3. Interleukin & Receptors
  4. IL-32
  5. Animal-Free IL-32 alpha Protein, Human (His)

Animal-Free IL-32 alpha Protein, Human (His)

Cat. No.: HY-P700124AF
COA Handling Instructions

IL-32 alpha Protein, a cytokine, potentially contributes to innate and adaptive immune responses. It induces TNFA/TNF-alpha and IL8, activating typical cytokine signal pathways, including NF-kappa-B and p38 MAPK. Animal-Free IL-32 alpha Protein, Human (His) is the recombinant human-derived animal-FreeIL-32 alpha protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-32 alpha Protein, Human (His) is 131 a.a., with molecular weight of ~15.72 kDa.

Animal-free recombinant proteins offer high consistency and stability, whithout using or contacting of any animal-derived materials and reagents.

For research use only. We do not sell to patients.

Protein Expression Service

Size Price Stock Quantity
2 μg $70 In-stock
10 μg $195 In-stock
50 μg $550 In-stock
100 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

IL-32 alpha Protein, a cytokine, potentially contributes to innate and adaptive immune responses. It induces TNFA/TNF-alpha and IL8, activating typical cytokine signal pathways, including NF-kappa-B and p38 MAPK. Animal-Free IL-32 alpha Protein, Human (His) is the recombinant human-derived animal-FreeIL-32 alpha protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-32 alpha Protein, Human (His) is 131 a.a., with molecular weight of ~15.72 kDa.

Background

IL-32 alpha protein, classified as a cytokine, is implicated in both innate and adaptive immune responses. This protein demonstrates the ability to induce the expression of various cytokines, including TNFA/TNF-alpha and IL8, thereby influencing the inflammatory milieu. Furthermore, the signaling pathways triggered by IL-32 alpha encompass the canonical pathways of NF-kappa-B and p38 MAPK, highlighting its involvement in orchestrating diverse cellular responses associated with immune regulation. This underscores the potential significance of IL-32 alpha in the intricate network of immune signaling cascades, contributing to the modulation of inflammatory and immune processes.

Biological Activity

Measure by its ability to induce TNF alpha secretion in RAW264.7 cells. The ED50 for this effect is <10 μg/mL.

Species

Human

Source

E. coli

Tag

C-His

Accession

P24001-4 (M1-K131)

Gene ID
Molecular Construction
N-term
IL-32α (M1-K131)
Accession # P24001-4
His
C-term
Synonyms
IL-32alpha; IL-32beta; IL-32delta; IL-32gamma; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd
AA Sequence

MCFPKVLSDDMKKLKARMHQAIERFYDKMQNAESGRGQVMSSLAELEDDFKEGYLETVAAYYEEQHPELTPLLEKERDGLRCRGNRSPVPDVEDPATEEPGESFCDKSYGAPRGDKEELTPQKCSEPQSSK

Molecular Weight

Approximately 15.72 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a solution containing 1X PBS, pH 8.0.

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Animal-Free IL-32 alpha Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Animal-Free IL-32 alpha Protein, Human (His)
Cat. No.:
HY-P700124AF
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