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  4. Animal-Free PGA5 Protein, Human (His)

Animal-Free PGA5 Protein, Human (His)

Cat. No.: HY-P700144AF
COA Handling Instructions

PGA5 protein, with broad substrate specificity, prefers cleaving phenylalanine and leucine bonds but also shows the ability to cleave various other bonds to some extent. Animal-Free PGA5 Protein, Human (His) is the recombinant human-derived animal-FreePGA5 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free PGA5 Protein, Human (His) is 388 a.a., with molecular weight of ~42.80 kDa.

Animal-free recombinant proteins offer high consistency and stability, whithout using or contacting of any animal-derived materials and reagents.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
2 μg $102 In-stock
10 μg $286 In-stock
50 μg $799 In-stock
100 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

PGA5 protein, with broad substrate specificity, prefers cleaving phenylalanine and leucine bonds but also shows the ability to cleave various other bonds to some extent. Animal-Free PGA5 Protein, Human (His) is the recombinant human-derived animal-FreePGA5 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free PGA5 Protein, Human (His) is 388 a.a., with molecular weight of ~42.80 kDa.

Background

PGA5 protein demonstrates a wide substrate specificity, with a preference for cleaving bonds involving phenylalanine and leucine. Notably, it exhibits the ability to cleave many other types of bonds to some extent.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

E. coli

Tag

C-His

Accession

P0DJD9 (M1-A388)

Gene ID
Molecular Construction
N-term
PGA5 (M1-A388)
Accession # P0DJD9
His
C-term
Synonyms
Pepsin A-5; EC:3.4.23.1; pepsinogen 5, group I (pepsinogen A); Pepsinogen A5; PGA5
AA Sequence

MKWLLLLGLVALSECIMYKVPLIRKKSLRRTLSERGLLKDFLKKHNLNPARKYFPQWEAPTLVDEQPLENYLDMEYFGTIGIGTPAQDFTVVFDTGSSNLWVPSVYCSSLACTNHNRFNPEDSSTYQSTSETVSITYGTGSMTGILGYDTVQVGGISDTNQIFGLSETEPGSFLYYAPFDGILGLAYPSISSSGATPVFDNIWNQGLVSQDLFSVYLSADDKSGSVVIFGGIDSSYYTGSLNWVPVTVEGYWQITVDSITMNGETIACAEGCQAIVDTGTSLLTGPTSPIANIQSDIGASENSDGDMVVSCSAISSLPDIVFTINGVQYPVPPSAYILQSEGSCISGFQGMNVPTESGELWILGDVFIRQYFTVFDRANNQVGLAPVA

Molecular Weight

Approximately 42.80 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Animal-Free PGA5 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Animal-Free PGA5 Protein, Human (His)
Cat. No.:
HY-P700144AF
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