APOBEC3A Protein, Human (His-SUMO)
Based on 1 Customer Validation
APOBEC3A protein is a DNA deaminase that limits the mobility of viruses, foreign DNA, and retrotransposons. APOBEC3A Protein, Human (His-SUMO) is the recombinant human-derived APOBEC3A protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
APOBEC3A protein is a DNA deaminase that limits the mobility of viruses, foreign DNA, and retrotransposons. APOBEC3A Protein, Human (His-SUMO) is the recombinant human-derived APOBEC3A protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.
APOBEC3A protein functions as a DNA deaminase, specifically a cytidine deaminase, exerting restriction activity against viruses, foreign DNA, and the mobility of retrotransposons. It demonstrates antiviral efficacy against adeno-associated virus (AAV) and human T-cell leukemia virus type 1 (HTLV-1), potentially impeding the mobility of both LTR and non-LTR retrotransposons. APOBEC3A selectively targets single-stranded DNA, exhibiting the capacity to deaminate both methylcytosine and cytosine in foreign DNA. Additionally, it can induce somatic hypermutation in both nuclear and mitochondrial DNA, suggesting its involvement in genomic diversity. Furthermore, APOBEC3A may contribute to the epigenetic regulation of gene expression through the active process of DNA demethylation, highlighting its multifaceted role in cellular defense mechanisms and potential impact on the epigenetic landscape.
Immobilized Human APOBEC3A at 2 μg/mL can bind Anti-APOBEC3A antibody. The ED50 for this effect is 2.25 ng/mL.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His;N-SUMO
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Accession
P31941-1 (M1-N199)
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Gene ID100913187 [NCBI]
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Molecular Construction
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N-term
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6*His-SUMO
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APOBEC3A (M1-N199)
Accession # P31941-1 -
C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
APOBEC3A; A3A; Apolipoprotein B MRNA Editing Enzyme Catalytic Subunit 3A; Probable DNA DC->DU-Editing Enzyme APOBEC-3A; PHRBN; Single-Stranded DNA Cytosine Deaminase; ARP3; APOBEC3A Transcript Variant 3; Apolipoprotein B MRNA Editing Enzyme, Catalytic Pol
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AA Sequence
MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTSVKMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSWGCAGEVRAFLQENTHVRLRIFAARIYDYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWDTFVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN
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Molecular Weight
Approximately 36-39 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 0.1% SKL.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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