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ATG5 Protein, Human

Customer Review

Based on 1 Customer Validation

ATG5 is an important participant in the formation of autophagy vesicles and binds to ATG12 through a ubiquitin-like system, which is critical for the lipidation of ATG8 family proteins. It plays a crucial role in mitochondrial quality control, affects cell longevity, and is critical for lymphocyte development, antigen processing, axonal morphology maintenance, adipocyte differentiation, primary ciliogenesis, and apoptotic processes. ATG5 Protein, Human is the recombinant human-derived ATG5 protein, expressed by E. coli , with tag free.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for ATG5 Protein, Human
  • ATG5 Protein, Human Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

ATG5 is an important participant in the formation of autophagy vesicles and binds to ATG12 through a ubiquitin-like system, which is critical for the lipidation of ATG8 family proteins. It plays a crucial role in mitochondrial quality control, affects cell longevity, and is critical for lymphocyte development, antigen processing, axonal morphology maintenance, adipocyte differentiation, primary ciliogenesis, and apoptotic processes. ATG5 Protein, Human is the recombinant human-derived ATG5 protein, expressed by E. coli , with tag free.

Background

ATG5, a key participant in autophagic vesicle formation, undergoes conjugation with ATG12 through a ubiquitin-like system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme. The resulting ATG12-ATG5 conjugate serves as an E3-like enzyme essential for lipidation of ATG8 family proteins and their association with vesicle membranes. Beyond its role in autophagy, ATG5 contributes to mitochondrial quality control post-oxidative damage, impacting cellular longevity. It plays a critical role in lymphocyte development, vital for the survival and proliferation of both B and T lymphocytes, and is necessary for optimal antigen processing and presentation for MHC II. Additionally, ATG5 is involved in maintaining axon morphology, normal adipocyte differentiation, and promoting primary ciliogenesis through autophagic pathways. Moreover, it may play a crucial role in apoptotic processes, occurring downstream of caspase activity, and participates in IFN-gamma-induced autophagic cell death through interaction with FADD.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag Tag Free
  • Accession

    Q9H1Y0-1 (M1-D275)

  • Gene ID
    9474  [NCBI]
  • Molecular Construction
    • N-term
    • ATG5 (M1-D275)
      Accession # Q9H1Y0-1
    • C-term
  • Protein Length

    Full Length of Isoform 1

  • Synonyms

    ATG5; APG5 (Autophagy 5, S. Cerevisiae)-Like; Prev. APG5L; Uncharacterized Protein DKFZp686F0183; ASP; ATG5 Autophagy Related 5 Homolog; Autophagy Protein 5; APG5 Autophagy 5-Like; HAPG5; ATG5 Variant 3; APG5; DKFZp686F0183; Apoptosis-Specific Protein; AP

  • AA Sequence

    MTDDKDVLRDVWFGRIPTCFTLYQDEITEREAEPYYLLLPRVSYLTLVTDKVKKHFQKVMRQEDISEIWFEYEGTPLKWHYPIGLLFDLLASSSALPWNITVHFKSFPEKDLLHCPSKDAIEAHFMSCMKEADALKHKSQVINEMQKKDHKQLWMGLQNDRFDQFWAINRKLMEYPAEENGFRYIPFRIYQTTTERPFIQKLFRPVAADGQLHTLGDLLKEVCPSAIDPEDGEKKNQVMIHGIEPMLETPLQWLSEHLSYPDNFLHISIIPQPTD

  • Predicted Molecular Mass

    32.4 kDa

  • Molecular Weight

    Approximately 33 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filter solution of 20 mM Tris-HCl, 0.2 M NaCl, 1 mM DTT, 40% glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

References

[1]. Shida Yousefi, et al. Calpain-mediated cleavage of Atg5 switches autophagy to apoptosis. Nat Cell Biol. 2006 Oct;8(10):1124-32. [Content Brief]

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • ATG5 Protein, Human Product Image
    1/16
  • Experimental Validation Results for ATG5 Protein, Human
  • ATG5 Protein, Human Product Image