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B2M/Beta-2 microglobulin Protein, Mouse (HEK293, Fc)

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Based on 1 Customer Validation

Beta-2 microglobulin (B2M) is vital in presenting antigens to the immune system through MHC class I molecules. It forms a heterodimer with an alpha chain to create MHC class I molecules. B2M also forms a heterotrimer with MR1 and a metabolite antigen, enhancing antigen presentation and immune recognition. B2M's molecular interactions facilitate immune system functioning and foreign substance recognition. B2M/Beta-2 microglobulin Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived B2M/Beta-2 microglobulin protein, expressed by HEK293 , with C-hFc labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for B2M/Beta-2 microglobulin Protein, Mouse (HEK293, Fc)
  • B2M/Beta-2 microglobulin Protein, Mouse (HEK293, Fc) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

Beta-2 microglobulin (B2M) is vital in presenting antigens to the immune system through MHC class I molecules. It forms a heterodimer with an alpha chain to create MHC class I molecules. B2M also forms a heterotrimer with MR1 and a metabolite antigen, enhancing antigen presentation and immune recognition. B2M's molecular interactions facilitate immune system functioning and foreign substance recognition. B2M/Beta-2 microglobulin Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived B2M/Beta-2 microglobulin protein, expressed by HEK293 , with C-hFc labeled tag.

Background

Beta-2 microglobulin (B2M) protein is a crucial component of the class I major histocompatibility complex (MHC), playing a key role in presenting peptide antigens to the immune system. It forms a heterodimer with an alpha chain, together comprising the major histocompatibility complex class I molecules. Additionally, B2M can form a heterotrimer with MR1 and a metabolite antigen, further contributing to antigen presentation and immune recognition processes. By participating in these molecular interactions, B2M helps in the proper functioning of the immune system and the recognition of foreign substances.

Verified Bioactivity

Immobilized Mouse B2M at 2 μg/mL (100 μL/well) can bind Anti-B2M antibody. The ED50 for this effect is 0.7127 μg/mL.

MCE Validation Data

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Immobilized Mouse B2M at 2 μg/mL (100 μL/well) can bind Anti-B2M antibody. The ED50 for this effect is 0.7127 μg/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-hFc
  • Accession

    P01887 (I21-M119)

  • Gene ID
    12010  [NCBI]
  • Molecular Construction
    • N-term
    • B2M (I21-M119)
      Accession # P01887
    • hFc
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    B2M; Beta 2-Microglobulin; Beta-2-Microglobulin; Beta-2-Microglobin; Beta Chain Of MHC Class I Molecules; AMYLD6; Beta 2-Microglobulin Protein; MHC1D4; Human Nkt Tcr Alpha Chain; IMD43; Human Nkt Tcr Beta Chain

  • AA Sequence

    IQKTPQIQVYSRHPPENGKPNILNCYVTQFHPPHIEIQMLKNGKKIPKVEMSDMSFSKDWSFYILAHTEFTPTETDTYACRVKHASMAEPKTVYWDRDM

  • Predicted Molecular Mass

    38.4 kDa

  • Molecular Weight

    Approximately 40-50 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immobilized Mouse B2M at 2 μg/mL (100 μL/well) can bind Anti-B2M antibody. The ED50 for this effect is 0.7127 μg/mL.

  • B2M/Beta-2 microglobulin Protein, Mouse (HEK293, Fc) Product Image
    1/16
  • Experimental Validation Results for B2M/Beta-2 microglobulin Protein, Mouse (HEK293, Fc)
  • B2M/Beta-2 microglobulin Protein, Mouse (HEK293, Fc) Product Image