BLMH/Bleomycin Hydrolase Protein, Mouse (His)

Bleomycin Hydrolase Protein (BLMH) is crucial for cellular defense, inactivating the antitumor drug Bleomycin (BLM) by hydrolyzing its carboxamide bond. This neutralizes BLM's cytotoxic effects, protecting normal and malignant cells. BLMH's exact physiological function is unknown, but its significance in maintaining cellular balance and defending against harmful agents is evident. BLMH/Bleomycin Hydrolase Protein, Mouse (His) is the recombinant mouse-derived BLMH/Bleomycin Hydrolase Protein, expressed by E. coli , with N-His labeled tag.

BLM hydrolase, also known as Bleomycin hydrolase, plays a crucial role in cellular defense mechanisms by catalyzing the inactivation of the antitumor drug Bleomycin (BLM), a glycopeptide. This enzymatic activity involves the hydrolysis of the carboxamide bond within the B-aminoalaninamide moiety of BLM, leading to its inactivation. This process serves to protect both normal and malignant cells from the toxic effects of BLM. While the precise physiological function of BLM hydrolase remains unclear, its ability to neutralize the cytotoxic impact of BLM highlights its significance in cellular homeostasis and defense against potentially harmful agents.

Measured by its ability to hydrolyze Met-AMC and the specific activity is 368 pmols/min/ug. (Activation description: The proenzyme needs to be activated in reducing buffer for an activated form.)

Mouse

E. coli

N-His

Q8R016 (N2-E455)

104184  [NCBI]

Partial

NNAGLNSEKVSALIQKLNSDPQFVLAQNVGTTHDLLDICLRRATVQGAQHVFQHVVPQEGKPVTNQKSSGRCWIFSCLNVMRLPFMKKFNIEEFEFSQSYLFFWDKVERCYFFLNAFVDTAQKKEPEDGRLVQYLLMNPTNDGGQWDMLVNIVEKYGVVPKKCFPESHTTEATRRMNDILNHKMREFCIRLRNLVHSGATKGEISSTQDAMMEEIFRVVCICLGNPPETFTWEYRDKDKNYHKIGPITPLQFYKEHVKPLFNMEDKICFVNDPRPQHKYNKLYTVDYLSNMVGGRKTLYNNQPIDFLKKMVAASIKDGEAVWFGCDVGKHFNGKLGLSDMNVYDHELVFGVSLKNMNKAERLAFGESLMTHAMTFTAVSEKDNQEGTFVKWRVENSWGEDHGHKGYLCMTDEWFSEYVYEVVVDKKHVPEEVLAVLEQEPIVLPAWDPMGALAE

Approximately 47 kDa.

• 
  ≥ 90%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM Tris, 0.15M NaCl, 10% glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.