1. Recombinant Proteins
  2. Immune Checkpoint Proteins Biotinylated Proteins
  3. Butyrophilins
  4. BTN3A1/CD277
  5. BTN3A1/CD277 Protein, Human (Biotinylated, HEK293, Fc-Avi)

BTN3A1/CD277 Protein, Human (Biotinylated, HEK293, Fc-Avi)

Cat. No.: HY-P72345
Handling Instructions

BTN3A1/CD277 Protein, functioning as a homodimer, regulates activated T-cell proliferation, controls cytokine release, and mediates T-cell response to cells with elevated phosphorylated metabolites, such as isopentenyl pyrophosphate, contributing to T-cell activation and the adaptive immune response. BTN3A1/CD277 Protein, Human (Biotinylated, HEK293, Fc-Avi) is the recombinant human-derived BTN3A1/CD277 protein, expressed by HEK293 , with C-Avi, C-hFc labeled tag. The total length of BTN3A1/CD277 Protein, Human (Biotinylated, HEK293, Fc-Avi) is 225 a.a., with molecular weight of 58-62 kDa.

For research use only. We do not sell to patients.

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Description

BTN3A1/CD277 Protein, functioning as a homodimer, regulates activated T-cell proliferation, controls cytokine release, and mediates T-cell response to cells with elevated phosphorylated metabolites, such as isopentenyl pyrophosphate, contributing to T-cell activation and the adaptive immune response. BTN3A1/CD277 Protein, Human (Biotinylated, HEK293, Fc-Avi) is the recombinant human-derived BTN3A1/CD277 protein, expressed by HEK293 , with C-Avi, C-hFc labeled tag. The total length of BTN3A1/CD277 Protein, Human (Biotinylated, HEK293, Fc-Avi) is 225 a.a., with molecular weight of 58-62 kDa.

Background

The BTN3A1/CD277 protein is involved in crucial functions related to T-cell activation and the adaptive immune response. It acts as a regulator of activated T-cell proliferation and controls the release of cytokines and IFNG by these cells. Additionally, it plays a role in mediating the response of T-cells towards infected and transformed cells that exhibit elevated levels of phosphorylated metabolites, such as isopentenyl pyrophosphate. The BTN3A1/CD277 protein functions as a homodimer.

Species

Human

Source

HEK293

Tag

C-Avi;C-hFc

Accession

O00481 (Q30-G254)

Gene ID
Molecular Construction
N-term
BTN3A1 (Q30-G254)
Accession # O00481
hFc-Avi
C-term
Synonyms
Butyrophilin subfamily 3 member A1; CD277; BTN3A1; BTF5
AA Sequence

QFSVLGPSGPILAMVGEDADLPCHLFPTMSAETMELKWVSSSLRQVVNVYADGKEVEDRQSAPYRGRTSILRDGITAGKAALRIHNVTASDSGKYLCYFQDGDFYEKALVELKVAALGSDLHVDVKGYKDGGIHLECRSTGWYPQPQIQWSNNKGENIPTVEAPVVADGVGLYAVAASVIMRGSSGEGVSCTIRSSLLGLEKTASISIADPFFRSAQRWIAALAG

Molecular Weight

58-62 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

BTN3A1/CD277 Protein, Human (Biotinylated, HEK293, Fc-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
BTN3A1/CD277 Protein, Human (Biotinylated, HEK293, Fc-Avi)
Cat. No.:
HY-P72345
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