1. Recombinant Proteins
  2. Complement System
  3. Complement Component 7
  4. C7/Complement component C7 Protein, Human (HEK293, Fc)

C7/Complement component C7 Protein, Human (HEK293, Fc)

Cat. No.: HY-P74372
COA Handling Instructions

C7, also known as complement component C7 protein, is a key component of the membrane attack complex (MAC) and plays a key role in innate and adaptive immune responses. As a component of MAC, C7 actively participates in the formation of target cell plasma membrane pores. C7/Complement component C7 Protein, Human (HEK293, Fc) is the recombinant human-derived C7/Complement component C7 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of C7/Complement component C7 Protein, Human (HEK293, Fc) is 843 a.a., with molecular weight of ~118 kDa.

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Description

C7, also known as complement component C7 protein, is a key component of the membrane attack complex (MAC) and plays a key role in innate and adaptive immune responses. As a component of MAC, C7 actively participates in the formation of target cell plasma membrane pores. C7/Complement component C7 Protein, Human (HEK293, Fc) is the recombinant human-derived C7/Complement component C7 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of C7/Complement component C7 Protein, Human (HEK293, Fc) is 843 a.a., with molecular weight of ~118 kDa.

Background

Complement component C7 is an integral part of the membrane attack complex (MAC), a crucial effector in the innate and adaptive immune responses. The MAC plays a pivotal role by forming pores in the plasma membrane of target cells, leading to cell lysis and destruction. C7 functions as a membrane anchor in this process, and it can exist as a monomer or dimer, with the potential to form multimeric rosettes when part of the C5b-7 complex. MAC assembly begins with the proteolytic cleavage of C5 into C5a and C5b, and subsequently, C5b binds sequentially to C6, C7, C8, and multiple copies of the pore-forming subunit C9. This orchestrated sequence highlights the essential role of C7 in the assembly of MAC, contributing to the immune system's ability to eliminate target cells.

Species

Human

Source

HEK293

Tag

C-hFc

Accession

P10643 (M1-Q843)

Gene ID

730  [NCBI]

Molecular Construction
N-term
C7 (M1-Q843)
Accession # P10643
hFc
C-term
Synonyms
Complement component C7; C7
Molecular Weight

Approximately 118 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of PBS, 5 % Trehalose, 8% Glycerol, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

C7/Complement component C7 Protein, Human (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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C7/Complement component C7 Protein, Human (HEK293, Fc)
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HY-P74372
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