Carboxypeptidase A2/CPA2 Protein, Human (HEK293, His)
Carboxypeptidase A2/CPA2 Protein, Human (HEK293, His) is an approximately 50.0 kDa human carboxypeptidase A2 with a His-flag. Carboxypeptidase A2 is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
Carboxypeptidase A2/CPA2 Protein, Human (HEK293, His) is an approximately 50.0 kDa human carboxypeptidase A2 with a His-flag. Carboxypeptidase A2 is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides.
Background
There exists different forms of human pancreatic procarboxypeptidase A, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties[1].The hydrolytic action of CPA2 prefers towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide[2].Carboxypeptidase A2 is a secreted pancreatic procarboxy-peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group[1][2].
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
AAP36067.1 (L17-Y417)
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Molecular Construction
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N-term
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CPA2 (L17-Y417)
Accession # AAP36067.1 -
6*His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
CPA2; Carboxypeptidase A2 Isoform 4; Carboxypeptidase A2; Carboxypeptidase A2 Isoform 1; Carboxypeptidase A2 (Pancreatic); Carboxypeptidase A2 Isoform 5
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AA Sequence
LETFVGDQVLEIVPSNEEQIKNLLQLEAQEHLQLDFWKSPTTPGETAHVRVPFVNVQAVKVFLGSQGIAYSIMIEDVQVLLDKENEEMLFNRRRERSGNFNFGAYHTLEEISQEMDNLVAEHPGLVSKVNIGSSFENRPMNVLKFSTGGDKPAIWLDAGIHAREWVTQATALWTANKIVSDYGKDPSITSILDALDIFLLPVTNPDGYVFSQTKNRMWRKTRSKVSGSLCVGVDPNRNWDAGFGGPGASSNPCSDSYHGPSANSEVEVKSIVDFIKSHGKVKAFITLHSYSQLLMFPYGYKCTKLDDFDELSEVAQKAAQSLRSLHGTKYKVGPICSVIYQASGGSIDWSYDYGIKYSFAFELRDTGRYGFLLPARQILPTAEETWLGLKAIMEHVRDHPY
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Predicted Molecular Mass
45.9 kDa
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Molecular Weight
Approximately 50 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
References
[1]. L Catasús, et al. The sequence and conformation of human pancreatic procarboxypeptidase A2. cDNA cloning, sequence analysis, and three-dimensional model. J Biol Chem [Content Brief]
[2]. P Aloy, et al. Comparative analysis of the sequences and three-dimensional models of human procarboxypeptidases A1, A2 and B. Biol Chem. 1998 Feb;379(2):149-55. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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