Cathepsin L1 Protein, Human (HEK293, His)

Cathepsin L1 Protein, Human (HEK293, His) is a major cysteine protease that plays a major role in intracellular protein catabolism.

Cathepsin L1, a major cysteine protease secreted by the parasite plays a pivotal role in various aspects of its pathogenicity. For example, Cathepsin L1 takes part in nutrient acquisition by catabolizing host proteins to absorbable peptides, facilitates the migration of the parasite through the host intestine and liver by cleaving interstitial matrix proteins such as fibronectin, laminin, and native collagen, and is implicated in the inactivation of host immune defenses by cleaving immunoglobulins. Cathepsin L1 suppresses Th1 immune responses in infected laboratory animals making them susceptible to concurrent bacterial infections^[1].

Biological activity has been tested in vitro.

Human

HEK293

C-6*His

P07711 (T18-V333)

1514  [NCBI]

TLTFDHSLEAQWTKWKAMHNRLYGMNEEGWRRAVWEKNMKMIELHNQEYREGKHSFTMAMNAFGDMTSEEFRQVMNGFQNRKPRKGKVFQEPLFYEAPRSVDWREKGYVTPVKNQGQCGSCWAFSATGALEGQMFRKTGRLISLSEQNLVDCSGPQGNEGCNGGLMDYAFQYVQDNGGLDSEESYPYEATEESCKYNPKYSVANDTGFVDIPKQEKALMKAVATVGPISVAIDAGHESFLFYKEGIYFEPDCSSEDMDHGVLVVGYGFESTESDNNKYWLVKNSWGEEWGMGGYVKMAKDRRNHCGIASAASYPTVHHHHHH

Approximately 34 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filter solution of 20 mM HAc-NaAc, 150 mM NaCl, pH 4.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Panat Anuracpreeda, et al. Production and characterization of a monoclonal antibody against recombinant cathepsin L1 of Fasciola gigantica. Acta Trop. 2014 Aug;136:1-9.  [Content Brief]