1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Hydrolases (EC 3) Cathepsin
  4. Cathepsin L1
  5. Cathepsin L1 Protein, Human (HEK293, His, solution)

Cathepsin L1 Protein, Human (HEK293, His, solution)

Cat. No.: HY-P7754
COA Handling Instructions

Cathepsin L1 Protein, Human (HEK293, His, solution) is a major cysteine protease that plays a major role in intracellular protein catabolism.

For research use only. We do not sell to patients.

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10 μg $140 In-stock
50 μg $420 In-stock
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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

  • References

Description

Cathepsin L1 Protein, Human (HEK293, His, solution) is a major cysteine protease that plays a major role in intracellular protein catabolism.

Background

Cathepsin L1, a major cysteine protease secreted by the parasite plays a pivotal role in various aspects of its pathogenicity. For example, Cathepsin L1 takes part in nutrient acquisition by catabolizing host proteins to absorbable peptides, facilitates the migration of the parasite through the host intestine and liver by cleaving interstitial matrix proteins such as fibronectin, laminin, and native collagen, and is implicated in the inactivation of host immune defenses by cleaving immunoglobulins. Cathepsin L1 suppresses Th1 immune responses in infected laboratory animals making them susceptible to concurrent bacterial infections[1].

Biological Activity

Biological activity has been tested in vitro.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

P07711 (T18-V333)

Gene ID
Molecular Construction
N-term
Cathepsin L1 (T18-V333)
Accession # P07711
6*His
C-term
Synonyms
rHuCathepsin L1, His; Cathepsin L1; Major Excreted Protein; MEP; CTSL1; CTSL
AA Sequence

TLTFDHSLEAQWTKWKAMHNRLYGMNEEGWRRAVWEKNMKMIELHNQEYREGKHSFTMAMNAFGDMTSEEFRQVMNGFQNRKPRKGKVFQEPLFYEAPRSVDWREKGYVTPVKNQGQCGSCWAFSATGALEGQMFRKTGRLISLSEQNLVDCSGPQGNEGCNGGLMDYAFQYVQDNGGLDSEESYPYEATEESCKYNPKYSVANDTGFVDIPKQEKALMKAVATVGPISVAIDAGHESFLFYKEGIYFEPDCSSEDMDHGVLVVGYGFESTESDNNKYWLVKNSWGEEWGMGGYVKMAKDRRNHCGIASAASYPTVHHHHHH

Molecular Weight

Approximately 34 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filter solution of 20 mM HAc-NaAc, 150 mM NaCl, pH 4.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
References

Cathepsin L1 Protein, Human (HEK293, His, solution) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Cathepsin L1 Protein, Human (HEK293, His, solution)
Cat. No.:
HY-P7754
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