CCT2 Protein, Human (His-SUMO)

The CCT2 protein is an important component of the chaperone-containing T complex (TRiC), a molecular chaperone that assists in protein folding through ATP hydrolysis. CCT2 Protein, Human (His-SUMO) is the recombinant human-derived CCT2 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of CCT2 Protein, Human (His-SUMO) is 534 a.a., with molecular weight of ~66 kDa.

CCT2 operates as an integral component of the chaperonin-containing T-complex (TRiC), a sophisticated molecular chaperone complex pivotal for protein folding through ATP hydrolysis. This versatile complex plays a crucial role in mediating the folding of various proteins, including WRAP53/TCAB1, thus influencing telomere maintenance. Furthermore, as part of the TRiC complex, CCT2 may contribute to the assembly of the BBSome, a complex essential for ciliogenesis that regulates the transport of vesicles to cilia. The TRiC complex's involvement in the folding of actin and tubulin underscores its multifaceted functions in cellular processes (Probable). Structurally, CCT2 is a key constituent of the TRiC complex, a heterooligomeric assembly characterized by two stacked rings, each measuring 12 to 16 nm in diameter. Beyond its structural role, CCT2 engages in interactions with various proteins, including PACRG, FLCN, and DLEC1, further highlighting its significance in diverse cellular processes.

Human

E. coli

N-6*His;N-SUMO

P78371 (A2-C535)

10576  [NCBI]

ASLSLAPVNIFKAGADEERAETARLTSFIGAIAIGDLVKSTLGPKGMDKILLSSGRDASLMVTNDGATILKNIGVDNPAAKVLVDMSRVQDDEVGDGTTSVTVLAAELLREAESLIAKKIHPQTIIAGWREATKAAREALLSSAVDHGSDEVKFRQDLMNIAGTTLSSKLLTHHKDHFTKLAVEAVLRLKGSGNLEAIHIIKKLGGSLADSYLDEGFLLDKKIGVNQPKRIENAKILIANTGMDTDKIKIFGSRVRVDSTAKVAEIEHAEKEKMKEKVERILKHGINCFINRQLIYNYPEQLFGAAGVMAIEHADFAGVERLALVTGGEIASTFDHPELVKLGSCKLIEEVMIGEDKLIHFSGVALGEACTIVLRGATQQILDEAERSLHDALCVLAQTVKDSRTVYGGGCSEMLMAHAVTQLANRTPGKEAVAMESYAKALRMLPTIIADNAGYDSADLVAQLRAAHSEGNTTAGLDMREGTIGDMAILGITESFQVKRQVLLSAAEAAEVILRVDNIIKAAPRKRVPDHHPC

Approximately 66 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.