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CCT2 Protein, Human (His-SUMO)

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The CCT2 protein is an important component of the chaperone-containing T complex (TRiC), a molecular chaperone that assists in protein folding through ATP hydrolysis. CCT2 Protein, Human (His-SUMO) is the recombinant human-derived CCT2 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • CCT2 Protein, Human (His-SUMO) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The CCT2 protein is an important component of the chaperone-containing T complex (TRiC), a molecular chaperone that assists in protein folding through ATP hydrolysis. CCT2 Protein, Human (His-SUMO) is the recombinant human-derived CCT2 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.

Background

CCT2 operates as an integral component of the chaperonin-containing T-complex (TRiC), a sophisticated molecular chaperone complex pivotal for protein folding through ATP hydrolysis. This versatile complex plays a crucial role in mediating the folding of various proteins, including WRAP53/TCAB1, thus influencing telomere maintenance. Furthermore, as part of the TRiC complex, CCT2 may contribute to the assembly of the BBSome, a complex essential for ciliogenesis that regulates the transport of vesicles to cilia. The TRiC complex's involvement in the folding of actin and tubulin underscores its multifaceted functions in cellular processes (Probable). Structurally, CCT2 is a key constituent of the TRiC complex, a heterooligomeric assembly characterized by two stacked rings, each measuring 12 to 16 nm in diameter. Beyond its structural role, CCT2 engages in interactions with various proteins, including PACRG, FLCN, and DLEC1, further highlighting its significance in diverse cellular processes.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-6*His;N-SUMO
  • Accession

    P78371-1 (A2-C535)

  • Gene ID
    10576  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His-SUMO
    • CCT2 (A2-C535)
      Accession # P78371-1
    • C-term
  • Protein Length

    Full Length of Isoform-1 Mature Protein

  • Synonyms

    CCT2; Chaperonin Containing T-Complex Polypeptide 1, Beta Subunit; Chaperonin Containing TCP1 Subunit 2; Chaperonin Containing T-Complex Polypeptide 1, Subunit 2; T-Complex Protein 1 Subunit Beta; Chaperonin Containing T-Complex Polypeptide 1 Subunit 2; C

  • AA Sequence

    ASLSLAPVNIFKAGADEERAETARLTSFIGAIAIGDLVKSTLGPKGMDKILLSSGRDASLMVTNDGATILKNIGVDNPAAKVLVDMSRVQDDEVGDGTTSVTVLAAELLREAESLIAKKIHPQTIIAGWREATKAAREALLSSAVDHGSDEVKFRQDLMNIAGTTLSSKLLTHHKDHFTKLAVEAVLRLKGSGNLEAIHIIKKLGGSLADSYLDEGFLLDKKIGVNQPKRIENAKILIANTGMDTDKIKIFGSRVRVDSTAKVAEIEHAEKEKMKEKVERILKHGINCFINRQLIYNYPEQLFGAAGVMAIEHADFAGVERLALVTGGEIASTFDHPELVKLGSCKLIEEVMIGEDKLIHFSGVALGEACTIVLRGATQQILDEAERSLHDALCVLAQTVKDSRTVYGGGCSEMLMAHAVTQLANRTPGKEAVAMESYAKALRMLPTIIADNAGYDSADLVAQLRAAHSEGNTTAGLDMREGTIGDMAILGITESFQVKRQVLLSAAEAAEVILRVDNIIKAAPRKRVPDHHPC

  • Predicted Molecular Mass

    70.3 kDa

  • Molecular Weight

    Approximately 66 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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