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CD161 Protein, Human (Biotinylated, HEK293, His-Avi)

Customer Review

Based on 1 Customer Validation

CD161 protein plays a crucial role in inhibiting NK cell toxicity by activating specific acid sphingomyelinase/SMPD1 and increasing ceramide levels. Activation stimulates AKT1/PKB and RPS6KA1/RSK1 kinases, enhancing anti-CD3-induced T cell proliferation. CD161 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CD161 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CD161 Protein, Human (Biotinylated, HEK293, His-Avi)
  • CD161 Protein, Human (Biotinylated, HEK293, His-Avi) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

CD161 protein plays a crucial role in inhibiting NK cell toxicity by activating specific acid sphingomyelinase/SMPD1 and increasing ceramide levels. Activation stimulates AKT1/PKB and RPS6KA1/RSK1 kinases, enhancing anti-CD3-induced T cell proliferation. CD161 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CD161 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

Background

CD161 Protein assumes a crucial role in inhibiting natural killer (NK) cell cytotoxicity. Upon activation, CD161 stimulates specific acid sphingomyelinase/SMPD1, resulting in a significant increase in intracellular ceramide levels. The activation process also leads to the stimulation of AKT1/PKB and RPS6KA1/RSK1 kinases, along with a marked enhancement of T-cell proliferation induced by anti-CD3. Functioning as a lectin, CD161 binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope and the N-acetyllactosamine epitope. Furthermore, it acts as a ligand for CLEC2D/LLT1, inhibiting NK cell-mediated cytotoxicity and interferon-gamma secretion in target cells. Existing as a homodimer with disulfide linkage, CD161 interacts with acid sphingomyelinase/SMPD1, contributing to its multifaceted regulatory functions in immune responses.

Verified Bioactivity

1.Immobilized Biotinylated Human CD161 His at 1 μg/mL (100 μL/Well) on the plate. Dose response curve for Anti-CD161 Antibody hFc with the EC50 < 9.3 ng/mL determined by ELISA.
2.Immobilized Ant-CD161 Anlibody,hFc Tag at 5 μg/mL (100 μL/Well)on the plate. Dose response curve for Biotinylated Human CD161,His Tag with the EC50 ≤ 28.9 ng/mL detormined by ELISA.
3. Loaded Rezorstobart (HY-P990760) on AHC2 biosensor, can bind CD161 Protein, Human (Biotinylated, HEK293, His-Avi) with an affinity constant of 3.766E-10 M as determined in BLI assay.

MCE Validation Data

  • Bioactivity - BLI

    Bioactivity - BLI

    Loaded Rezorstobart (HY-P990760) on AHC2 biosensor, can bind CD161 Protein, Human (Biotinylated, HEK293, His-Avi) with an affinity constant of 3.766E-10 M as determined in BLI assay.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-Avi;C-8*His
  • Accession

    Q12918 (Q67-S225)

  • Gene ID
    3820  [NCBI]
  • Molecular Construction
    • N-term
    • CD161 (Q67-S225)
      Accession # Q12918
    • 8*His-Avi
    • C-term
  • Protein Length

    Extracellular Domain

  • Conjugation

    Biotin

  • Synonyms

    KLRB1; CD161; Prev. NKR; Killer Cell Lectin-Like Receptor Subfamily B Member 1; NKR-P1A; C-Type Lectin Domain Family 5 Member B; CLEC5B; NKRP1A; Natural Killer Cell Surface Protein P1A; CD161 Antigen; HNKR-P1A; HNKR-P1a; NKR-P1; Killer Cell Lectin Like Re

  • AA Sequence

    QKSSIEKCSVDIQQSRNKTTERPGLLNCPIYWQQLREKCLLFSHTVNPWNNSLADCSTKESSLLLIRDKDELIHTQNLIRDKAILFWIGLNFSLSEKNWKWINGSFLNSNDLEIRGDAKENSCISISQTSVYSEYCSTEIRWICQKELTPVRNKVYPDS

  • Predicted Molecular Mass

    21.4 kDa

  • Molecular Weight

    Approximately 41-45 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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    MedChemExpress Validation
     Loaded Rezorstobart (HY-P990760) on AHC2 biosensor, can bind CD161 Protein, Human (Biotinylated, HEK293, His-Avi) with an affinity constant of 3.766E-10 M as determined in BLI assay.

  • CD161 Protein, Human (Biotinylated, HEK293, His-Avi) Product Image
    1/16
  • Experimental Validation Results for CD161 Protein, Human (Biotinylated, HEK293, His-Avi)
  • CD161 Protein, Human (Biotinylated, HEK293, His-Avi) Product Image