CD162/PSGL-1 Protein, Mouse (HEK293, Fc)
Based on 1 Customer Validation
CD162/PSGL-1 Protein, Mouse (HEK293, Fc) is a recombinant mouse CD162 expressed in HEK 293 cells with a C-Fc tag at the C-terminus. CD162/PSGL-1 Protein plays an important role in immunity and homeostasis .
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
CD162/PSGL-1 Protein, Mouse (HEK293, Fc) is a recombinant mouse CD162 expressed in HEK 293 cells with a C-Fc tag at the C-terminus. CD162/PSGL-1 Protein plays an important role in immunity and homeostasis [1][2].
Background
PSGL-1 (P-Selectin Glycoprotein Ligand-1, designated CD162), is a type I membrane protein. PSGL-1 is expressed as a disulfide-linked homodimer with an extended mucin ectodomain composed of serine, threonine, and proline-rich decameric repeats bearing sites for potential O-glycan modification. PSGL-1 is expressed on all myeloid and lymphoid lineages including dendritic cells (DCs) and is downregulated on lymphocytes after entry into secondary lymphoid organs. Although not originally observed in megakaryocytes, PSGL-1 expression was identified in platelets with expression further increased in inflammatory settings[1][2].
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-Fc
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Accession
CAA62583.1 (Q42-C307)
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Molecular Construction
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N-term
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CD162/PSGL-1 (Q42-C307)
Accession # CAA62583.1 -
Fc
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C-term
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Protein Length
Partial Extracellular Domain
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Synonyms
SELPLG; Cutaneous Lymphocyte-Associated Associated Antigen; Selectin P Ligand; CD162 Antigen; PSGL-1; PSGL1; CD162; CLA; P-Selectin Glycoprotein Ligand 1
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AA Sequence
QVVGDDDFEDPDYTYNTDPPELLKNVTNTVAAHPELPTTVVMLERDSTSAGTSERATEKIATTDPTAPGTGGTAVGMLSTDSATQWSLTSVETVQPASTEVETSQPAPMEAETSQPAPMEAETSQPAPMEADTSKPAPTEAETSKPAPTEAETSQPAPNEAETSKPAPTEAETSKPAPTEAETTQLPRIQAVKTLFTTSAATEVPSTEPTTMETASTESNESTIFLGPSVTHLPDSGLKKGLIVTPGNSPAPTLPGSSDLIPVKQC
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Predicted Molecular Mass
54.7 kDa
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Molecular Weight
Approximately 90 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (263 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Yang J, et, al. The biology of P-selectin glycoprotein ligand-1: its role as a selectin counterreceptor in leukocyte-endothelial and leukocyte-platelet interaction. Thromb Haemost. 1999 Jan;81(1):1-7. [Content Brief]
[2]. Carlow DA, et, al. PSGL-1 function in immunity and steady state homeostasis. Immunol Rev. 2009 Jul;230(1):75-96. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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