CD20/MS4A1 Protein-Nanodisc, Human (HEK293, His)
Based on 1 Customer Validation
CD20/MS4A1 Protein-Nanodisc, Human (HEK293, His) is produced using our proprietary Nanodisc platform. This platform employs styrene-maleic acid (SMA) copolymer technology, which enables detergent-free, direct extraction of membrane proteins from both prokaryotic and eukaryotic expression systems. The SMA copolymer integrates into the lipid bilayer and excises ~10 nm membrane discs that contain the embedded target protein, effectively preserving its native conformation and biological activity. The resulting lipid–protein complexes can be readily purified by standard chromatographic methods.Compared with conventional membrane scaffold protein (MSP)-based nanodiscs, SMA nanodiscs maintain the native lipid environment surrounding the target protein without introducing exogenous protein scaffolds. This offers a more physiologically relevant context for structural and functional characterization, and provides a distinct advantage in membrane protein functional screening and drug discovery applications.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
CD20/MS4A1 Protein-Nanodisc, Human (HEK293, His) is produced using our proprietary Nanodisc platform. This platform employs styrene-maleic acid (SMA) copolymer technology, which enables detergent-free, direct extraction of membrane proteins from both prokaryotic and eukaryotic expression systems. The SMA copolymer integrates into the lipid bilayer and excises ~10 nm membrane discs that contain the embedded target protein, effectively preserving its native conformation and biological activity. The resulting lipid–protein complexes can be readily purified by standard chromatographic methods.Compared with conventional membrane scaffold protein (MSP)-based nanodiscs, SMA nanodiscs maintain the native lipid environment surrounding the target protein without introducing exogenous protein scaffolds. This offers a more physiologically relevant context for structural and functional characterization, and provides a distinct advantage in membrane protein functional screening and drug discovery applications.
Background
The CD20/MS4A1 protein, a B-lymphocyte-specific membrane protein, plays a crucial role in regulating cellular calcium influx essential for the development, differentiation, and activation of B-lymphocytes. It functions as a component of the store-operated calcium (SOC) channel, promoting calcium influx upon activation by the B-cell receptor/BCR. CD20/MS4A1 forms homotetramers, contributing to its structural organization and functional role in calcium signaling. Notably, it interacts with both the heavy and light chains of cell surface IgM, the antigen-binding components of the BCR, highlighting its involvement in the B-cell receptor complex and underscoring its significance in B-cell activation and immune responses.
Verified Bioactivity
1.Immobilized Rituximab (HY-P9913) at 2 μg/mL (100 μL/well) can bind Human CD20 Full Length. The ED50 for this effect is 0.969 ng/mL.
2.Immobilized Human CD20 Full Length, His Tag at 5 μg/mL (100 μL/well) can bind Biosimilar of Obinutuzumab (HY-P9910), The ED50 for this effect is 8.78 ng/mL.
3.Human CD20 Full Length, His Tag captured on CM5 Chip via anti-His antibody can bind Rituximab(HY-P9913) with an affinity constant of 75.2 nM as determined in SPR assay.
4.Human CD20 Full Length, His Tag captured on CM5 Chip via anti-His antibody can bind Obinutuzumab(HY-P9910) with an affinity constant of 27.6 nM as determined in SPR assay.
5.Human CD20 Full Length, His Tag captured on CM5 Chip via anti-His antibody can bind Ofatumumab(HY-P9961) with an affinity constant of 6.1 nM as determined in SPR assay.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
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Bioactivity - ELISA
Bioactivity - ELISA
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Bioactivity - ELISA
Bioactivity - ELISA
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Bioactivity - SPR
Bioactivity - SPR
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Bioactivity - SPR
Bioactivity - SPR
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Bioactivity - SPR
Bioactivity - SPR
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
P11836-1 (M1-P297)
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Gene ID931
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Molecular Construction
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N-term
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CD20 (M1-P297)
Accession # P11836-1 -
6*His
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C-term
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Protein Length
Full Length
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Synonyms
MS4A1; CD20 Antigen; Prev. CD20; B-Lymphocyte Cell-Surface Antigen B1; Bp35; B-Lymphocyte Surface Antigen B1; FMC7; CD20 Receptor; B1; LEU-16; Membrane-Spanning 4-Domains, Subfamily A, Member 1; CVID5; Leukocyte Surface Antigen Leu-16; S7; B-Lymphocyte An
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AA Sequence
MTTPRNSVNGTFPAEPMKGPIAMQSGPKPLFRRMSSLVGPTQSFFMRESKTLGAVQIMNGLFHIALGGLLMIPAGIYAPICVTVWYPLWGGIMYIISGSLLAATEKNSRKCLVKGKMIMNSLSLFAAISGMILSIMDILNIKISHFLKMESLNFIRAHTPYINIYNCEPANPSEKNSPSTQYCYSIQSLFLGILSVMLIFAFFQELVIAGIVENEWKRTCSRPKSNIVLLSAEEKKEQTIEIKEEVVGLTETSSQPKNEEDIEIIPIQEEEEEETETNFPEPPQDQESSPIENDSSP
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Predicted Molecular Mass
33.9 kDa
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Molecular Weight
Approximately 35 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution in PBS, pH 7.4, 8% trehalose.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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