Rituximab (Synonyms: Anti-Human CD20 type I, Chimeric Antibody; HSDB 7455; IDEC-C2B8)

Name: Rituximab
Brand: MedChemExpress
SKU: HY-P9913
Rating: 5.0 (12 reviews)

Cat. No.: HY-P9913 Purity: 99.50%: Data Sheet
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Rituximab is an anti-CD20 chimeric monoclonal antibody used for research of certain autoimmune diseases and cancer.

For research use only. We do not sell to patients.

CAS No. : 174722-31-7

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1 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 12 publication(s) in Google Scholar

Other Forms of Rituximab:

Rituximab (anti-CD20) In-stock

12 Publications Citing Use of MCE Rituximab

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Bio/Physico-chemical Assay

: Rituximab purchased from MedChemExpress. Usage Cited in: Clin Transl Med. 2025 May;15(5):e70336. [Abstract]; DLBCL cells were treated either with ABT-199, ABT-737, or R-CHOP (CHOP plus 10 µg/mL Rituximab) for 48 h.

: Rituximab purchased from MedChemExpress. Usage Cited in: Electrophoresis. 2025 Sep 23. [Abstract]; Overlay of CZE electropherograms of rituximab (1 mg/mL) and infliximab analysed using 400 mM eACA, 2 mM TETA, 0.05% HPMC, pH 5.7 (blue), 400 mM GABA, 2 mM TETA, 0.05% HPMC, pH 5.7 (red), 400 mM 7-AHA, 2 mM TETA, 0.05% HPMC, and pH 5.7 (green).

: Rituximab purchased from MedChemExpress. Usage Cited in: EBioMedicine. 2024 Nov:109:105379. [Abstract]; Biological activity of ADCC response induced by trastuzumab in BT474 and SKBR3 cells upon treatment with increasing concentrations of rituximab (6 h) was measured.

: Rituximab purchased from MedChemExpress. Usage Cited in: Research Square Preprint. 2022 Mar.; In the case of rituximab (17 nM; 48 h) killing, flow cytometry detected the proportion of cells in the G2 phase of each group.

: Rituximab purchased from MedChemExpress. Usage Cited in: Cancer Biol Med. 2020 Nov 15;17(4):1026-1038. [Abstract]; Namalwa cells and Daudi cells were mixed with phosphate-buffered saline, rituximab (100 nM), or 161519 TriKE (100 nM) and incubated for 30 min at 37 °C.

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Description

Rituximab is an anti-CD20 chimeric monoclonal antibody used for research of certain autoimmune diseases and cancer.

Isotype

Human IgG1 kappa

Recommend Isotype Controls

Human IgG1 kappa, Isotype Control

Species Reactivity

Human

IC₅₀ & Target

CD20/MS4A1

RDC Antibody

In Vitro

Rituximab inhibits the proliferation of stimulated human B cells, which is associated with a relative increase of B cells with an activated naive phenotype. Aside from this population shift, there are no major changes in phenotype or cytokine profile of the various B-cell subsets. B cells stimulated in the presence of rituximab induces stronger T-cell proliferation, compared to B cells stimulated in the absence of rituximab^[1]. All lymphoma cells tested are equally sensitive to antibody-dependent cell-mediated cytotoxicity (ADCC), antibody-mediated phagocytosis of tumor cells, and rituximab-induced apoptosis. Rituximab induces high CDC killing of follicular lymphoma cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

A single injection of rituximab or the murine anti-CD20 Ab 1F5, given i.p. 1 day after the tumor, cures 100% of the animals. Depletion of either NK cells or neutrophils or both in tumor-injected animals does not affect the therapeutic activity of the drug. Similarly, rituximab is able to eradicate tumor cells in athymic nude mice, suggesting that its activity is T cell independent^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Gene ID

931 [NCBI]

Accession

P11836

Application

ELISA, FACS, Functional assay

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

Molecular Weight

143.47 kDa

CAS No.

174722-31-7

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Rituximab]

Shipping

Shipping with dry ice.

Formulation

Please refer to the lot-specific COA for specific buffer information.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Format

Human IgG1 kappa

Biological Activity

Rituximab has a significant ADCC effect on Raji cells in a dose-dependent manner.The EC₅₀ for this effect is 6.56 and 3.25 ng/mL.

Purity & Documentation

Purity: 99.85%

Select Batch:

Data Sheet (262 KB) SDS (252 KB)

COA (291 KB)

Inhibitory Antibodies User Guide (603 KB)

References

[1]. Kamburova EG, et al. In vitro effects of rituximab on the proliferation, activation and differentiation of human B cells. Am J Transplant. 2012 Feb;12(2):341-50. [Content Brief]

[2]. Manches O, et al. In vitro mechanisms of action of rituximab on primary non-Hodgkin lymphomas. Blood. 2003 Feb 1;101(3):949-54. [Content Brief]

[3]. Byrd JC, et al. The mechanism of tumor cell clearance by rituximab in vivo in patients with B-cell chronic lymphocytic leukemia: evidence of caspase activation and apoptosis induction. Blood. 2002 Feb 1;99(3):1038-43. [Content Brief]

Cell Assay	A single injection of rituximab or the murine anti-CD20 Ab 1F5, given i.p. 1 day after the tumor, cures 100% of the animals. Depletion of either NK cells or neutrophils or both in tumor-injected animals does not affect the therapeutic activity of the drug. Similarly, rituximab is able to eradicate tumor cells in athymic nude mice, suggesting that its activity is T cell independent^[3]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[1]	C57BL/6 mice (8-10 wk of age) are inoculated 8×10³ EL4-CD20⁺ cells in 200 μL of saline by tail vein injection. In parallel groups of mice, 150 g of rituximab, murine anti-CD20 IgG2a Ab 1F5, or control anti-human IL-2R Ab daclizumab in 300 μL of saline, or saline only is inoculated i.p. 24 h later^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Kamburova EG, et al. In vitro effects of rituximab on the proliferation, activation and differentiation of human B cells. Am J Transplant. 2012 Feb;12(2):341-50. [Content Brief] [2]. Manches O, et al. In vitro mechanisms of action of rituximab on primary non-Hodgkin lymphomas. Blood. 2003 Feb 1;101(3):949-54. [Content Brief] [3]. Byrd JC, et al. The mechanism of tumor cell clearance by rituximab in vivo in patients with B-cell chronic lymphocytic leukemia: evidence of caspase activation and apoptosis induction. Blood. 2002 Feb 1;99(3):1038-43. [Content Brief]

Rituximab Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Rituximab174722-31-7Anti-Human CD20 type I, Chimeric Antibody HSDB 7455 IDEC-C2B8HSDB7455HSDB 7455HSDB-7455Radionuclide-Drug Conjugates (RDCs)CD20Inhibitorinhibitorinhibit

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Rituximab (Synonyms: Anti-Human CD20 type I, Chimeric Antibody; HSDB 7455; IDEC-C2B8)

Customer Review

Other Forms of Rituximab:

Rituximab Related Antibodies

12 Publications Citing Use of MCE Rituximab

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Radionuclide-Drug Conjugates (RDCs) Isoform Specific Products:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

Rituximab Related Antibodies

Rituximab Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

Your Recently Viewed Products:

Customer Review

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