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CD204/MSR1 Protein, Mouse (HEK293, His)

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Based on 1 publication(s) in Google Scholar

CD204/MSR1 Protein, a membrane glycoprotein, crucially contributes to cholesterol accumulation in arterial walls during atherogenesis. With two receptor subunits, it facilitates endocytosis of various macromolecules, including modified low-density lipoproteins (LDL). As a homotrimer, CD204/MSR1 engages in key interactions, including its association with MYO18A, highlighting its functional role in cellular processes. CD204/MSR1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD204/MSR1 protein, expressed by HEK293 , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CD204/MSR1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for CD204/MSR1 Protein, Mouse (HEK293, His)
  • CD204/MSR1 Protein, Mouse (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

CD204/MSR1 Protein, a membrane glycoprotein, crucially contributes to cholesterol accumulation in arterial walls during atherogenesis. With two receptor subunits, it facilitates endocytosis of various macromolecules, including modified low-density lipoproteins (LDL). As a homotrimer, CD204/MSR1 engages in key interactions, including its association with MYO18A, highlighting its functional role in cellular processes. CD204/MSR1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD204/MSR1 protein, expressed by HEK293 , with N-His labeled tag.

Background

CD204/MSR1 Protein, a membrane glycoprotein, plays a crucial role in the pathological accumulation of cholesterol in arterial walls during atherogenesis. Comprising two types of receptor subunits, this protein facilitates the endocytosis of a diverse array of macromolecules, including modified low-density lipoproteins (LDL). As a homotrimer, CD204/MSR1 engages in key interactions, such as its association with MYO18A, contributing to its functional role in cellular processes.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse CD204 at 5 µg/mL (100 µL/well) can bind biotinylated advanced glycation endproducts of bovine serum albumin (AGE-BSA). The ED50 for this effect is 92.81 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse CD204 at 5 µg/mL (100 µL/well) can bind biotinylated advanced glycation endproducts of bovine serum albumin (AGE-BSA) .The ED50 for this effect is 92.81 ng/mL

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag N-His
  • Accession

    AAH03814.1 (W83-V354)

  • Gene ID
    20288  [NCBI]
  • Molecular Construction
    • N-term
    • His
    • CD204/MSR1 (W83-V354)
      Accession # AAH03814.1
    • C-term
  • Protein Length

    Partial Extracellular Domain

  • Synonyms

    MSR1; Macrophage Acetylated LDL Receptor I And II; Macrophage Scavenger Receptor 1; Scavenger Receptor Class A Member 1; SCARA1; CDNA FLJ55770, Highly Similar To Macrophage Scavenger Receptor Types I And II; SR-AIII; Macrophage Scavenger Receptor Type III

  • AA Sequence

    WEMKNCLVCSRNTSDTSQGPMEKENTSNVEMRFTIIMAHMKDMEERIQSISNSKADLIDTGRFQNFSMATDQRLNDILLQLNSLILSVQEHGNSLDAISKSLQSLNMTLLDVQLHTETLHVRVRESTAKQQEDISKLEERVYKVSAEVQSVKEEQAHVEQEVKQEVRVLNNITNDLRLKDWEHSQTLKNITFIQGPPGPQGEKGDRGLTGQTGPPGAPGIRGIPGVKGDRGQIGFPGGRGNPGAPGKPGRSGSPGPKGQKGEKGSVGGSRSV

  • Molecular Weight

    Approximately 42-75 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse CD204 at 5 µg/mL (100 µL/well) can bind biotinylated advanced glycation endproducts of bovine serum albumin (AGE-BSA) .The ED50 for this effect is 92.81 ng/mL

  • CD204/MSR1 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for CD204/MSR1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for CD204/MSR1 Protein, Mouse (HEK293, His)
  • CD204/MSR1 Protein, Mouse (HEK293, His) Product Image