CD204/MSR1 Protein, Rat (HEK293, His)

CD204/MSR1 Protein is a homo-trimeric transmembrane glycoprotein, holds key inflammatory roles in multiple pathophysiologic processes. MSR1 is highly expressed in lung, arteries, and adipose tissue^[1]. CD204/MSR1 Protein, Rat (HEK293, His) is the recombinant rat-derived CD204/MSR1 protein, expressed by HEK293 , with N-His labeled tag.

MSR1, also known as scavenger receptor-A (SR-A) or cluster of differentiation 204 (CD204), holds key inflammatory roles in multiple pathophysiologic processes. Present primarily on the surface of various types of macrophage, this receptor variably affects processes such as atherosclerosis, innate and adaptive immunity, lung and liver disease, and more recently, cancer. MSR1 has been shown to be important for M2 polarisation. Therefore, the role it carries or MSR1 is in part determined by the type of macrophage in which MSR1 is expressed and location within which the macrophages reside. The role of MSR1 is often dichotomous, being either host protective or detrimental to the pathogenesis of disease^[1].

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat CD204 at 5 µg/mL (100 µL/well) can bind biotinylated advanced glycation endproducts of bovine serum albumin (AGE-BSA). The ED₅₀ for this effect is 303.8 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat CD204 at 5 µg/mL (100 µL/well) can bind biotinylated advanced glycation endproducts of bovine serum albumin (AGE-BSA) .The ED₅₀ for this effect is 303.8 ng/mL

Rat

HEK293

N-6*His

D3ZDS2 (W134-S509)

498638  [NCBI]

Extracellular Domain

WEMKNCLVGSANTSDTSQTLTGKENTSKAEMGFTIAMERMKALEERVQSISDSKAGLLDTDRFQNFSMATDQRLNDLLLQLNSLISSVQEHGESLDAISKSLQSLNTTLLDVQVHTETLNVKVRESTAKQQEDISKLEERVHKVSAEIQSVKEEQEHVEQEVKQEVKVLNNITNDLRLKDWEHSQTLKNITLIQGPPGPPGEKGDKGLTGQSGPPGVPGARGPPGPKGDQGPVGFPGGRGYPGAPGKAGRSGFPGPKGQKGEKGRAGISTSLKTVRLVDGSGPHEGRVEIFHEGQWGTICDDRWDIRAGQVVCRSLGYQDVLTVHKRAHFGQGTGPIWLNEVMCFGRESSIENCKISKWGELGCSHAEDAGVTCTS

Approximately 48-75 kDa due to the glycosylation.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Jack Gudgeon, et al. The role of macrophage scavenger receptor 1 (MSR1) in inflammatory disorders and cancer. Front Immunol. 2022 Oct 17:13:1012002.  [Content Brief]