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CD204/MSR1 Protein, Rat (HEK293, His)

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Based on 1 Customer Validation

CD204/MSR1 Protein is a homo-trimeric transmembrane glycoprotein, holds key inflammatory roles in multiple pathophysiologic processes. MSR1 is highly expressed in lung, arteries, and adipose tissue. CD204/MSR1 Protein, Rat (HEK293, His) is the recombinant rat-derived CD204/MSR1 protein, expressed by HEK293 , with N-His labeled tag.

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  • Species: Rat
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CD204/MSR1 Protein, Rat (HEK293, His)
  • Experimental Validation Results for CD204/MSR1 Protein, Rat (HEK293, His)
  • CD204/MSR1 Protein, Rat (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
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Biological Activity

Description

CD204/MSR1 Protein is a homo-trimeric transmembrane glycoprotein, holds key inflammatory roles in multiple pathophysiologic processes. MSR1 is highly expressed in lung, arteries, and adipose tissue[1]. CD204/MSR1 Protein, Rat (HEK293, His) is the recombinant rat-derived CD204/MSR1 protein, expressed by HEK293 , with N-His labeled tag.

Background

MSR1, also known as scavenger receptor-A (SR-A) or cluster of differentiation 204 (CD204), holds key inflammatory roles in multiple pathophysiologic processes. Present primarily on the surface of various types of macrophage, this receptor variably affects processes such as atherosclerosis, innate and adaptive immunity, lung and liver disease, and more recently, cancer. MSR1 has been shown to be important for M2 polarisation. Therefore, the role it carries or MSR1 is in part determined by the type of macrophage in which MSR1 is expressed and location within which the macrophages reside. The role of MSR1 is often dichotomous, being either host protective or detrimental to the pathogenesis of disease[1].

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat CD204 at 5 µg/mL (100 µL/well) can bind biotinylated advanced glycation endproducts of bovine serum albumin (AGE-BSA). The ED50 for this effect is 303.8 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat CD204 at 5 µg/mL (100 µL/well) can bind biotinylated advanced glycation endproducts of bovine serum albumin (AGE-BSA) .The ED50 for this effect is 303.8 ng/mL

Technical Parameters

  • Species Rat
  • Source HEK293
  • Tag N-6*His
  • Accession

    NP_001178868.1 (W134-S509)

  • Gene ID
    498638  [NCBI]
  • Molecular Construction
    • N-term
    • His
    • MSR1 (W134-S509)
      Accession # NP_001178868.1
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    MSR1; Macrophage Acetylated LDL Receptor I And II; Macrophage Scavenger Receptor 1; Scavenger Receptor Class A Member 1; SCARA1; CDNA FLJ55770, Highly Similar To Macrophage Scavenger Receptor Types I And II; SR-AIII; Macrophage Scavenger Receptor Type III

  • AA Sequence

    WEMKNCLVGSANTSDTSQTLTGKENTSKAEMGFTIAMERMKALEERVQSISDSKAGLLDTDRFQNFSMATDQRLNDLLLQLNSLISSVQEHGESLDAISKSLQSLNTTLLDVQVHTETLNVKVRESTAKQQEDISKLEERVHKVSAEIQSVKEEQEHVEQEVKQEVKVLNNITNDLRLKDWEHSQTLKNITLIQGPPGPPGEKGDKGLTGQSGPPGVPGARGPPGPKGDQGPVGFPGGRGYPGAPGKAGRSGFPGPKGQKGEKGRAGISTSLKTVRLVDGSGPHEGRVEIFHEGQWGTICDDRWDIRAGQVVCRSLGYQDVLTVHKRAHFGQGTGPIWLNEVMCFGRESSIENCKISKWGELGCSHAEDAGVTCTS

  • Molecular Weight

    Approximately 51-60 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Jack Gudgeon, et al. The role of macrophage scavenger receptor 1 (MSR1) in inflammatory disorders and cancer. Front Immunol. 2022 Oct 17:13:1012002. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat CD204 at 5 µg/mL (100 µL/well) can bind biotinylated advanced glycation endproducts of bovine serum albumin (AGE-BSA) .The ED50 for this effect is 303.8 ng/mL

  • CD204/MSR1 Protein, Rat (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for CD204/MSR1 Protein, Rat (HEK293, His)
  • Experimental Validation Results for CD204/MSR1 Protein, Rat (HEK293, His)
  • CD204/MSR1 Protein, Rat (HEK293, His) Product Image