CD43 Protein, Mouse (HEK293, His)

CD43, Mouse, a predominant leukocyte surface sialoprotein, crucially regulates T-cell functions. It positively influences T-cell activation, proliferation, differentiation, trafficking, and migration, especially enhancing migration to lymph nodes through ERM proteins. CD43, Mouse, negatively steers Th2 differentiation, promotes Th1 lineage commitment, and induces IFN-gamma expression during TCR activation in CD4(+) and CD8(+) T-cells. Facilitating Th17 cell rolling, CD43, Mouse, serves as a major E-selectin ligand, mediates Th17 cell adhesion to E-selectin, acts as a T-cell counter-receptor for SIGLEC1, and protects against apoptotic signals, promoting cell survival. CD43 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD43 protein, expressed by HEK293, with C-His labeled tag. The total length of CD43 Protein, Mouse (HEK293, His) is 229 a.a., with molecular weight of 80-120 kDa.

The CD43 protein in mice emerges as the predominant cell surface sialoprotein of leukocytes, wielding a regulatory influence over various T-cell functions crucial to immune responses. CD43 positively regulates T-cell activation, proliferation, differentiation, trafficking, and migration, particularly enhancing T-cell trafficking to lymph nodes through its interaction with ERM proteins (EZR, RDX, and MSN). In addition to negatively regulating Th2 cell differentiation, CD43 steers T-cells towards a Th1 lineage commitment. It plays a pivotal role in inducing the expression of IFN-gamma during T-cell receptor (TCR) activation, affecting both CD4(+) and CD8(+) T-cells, and contributes to T-cell preparation for cytokine sensing and differentiation into effector cells by influencing the expression of cytokine receptors IFNGR and IL4R. Serving as a major E-selectin ligand, CD43 facilitates Th17 cell rolling on activated vasculature and subsequent recruitment during inflammation, mediating Th17 cell adhesion to E-selectin. Moreover, CD43 acts as a T-cell counter-receptor for SIGLEC1 and exhibits a protective role by shielding cells from apoptotic signals, thus promoting cell survival.

Mouse

HEK293

C-His

P15702 (D20-G248)

20737  [NCBI]

80-120 kDa

Greater than 95% as determined by Tris-Bis PAGE.

<1 EU/μg, determined by LAL method.