CD47 Protein, Human (Biotinylated, HEK293, Fc-Avi)

FITC-Labeled CD47, an adhesive protein, modulates integrin signaling through G protein activation and serves as a receptor for thrombospondin THBS1, impacting signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, self-renewal, and immunoregulation. It regulates pulmonary endothelin EDN1 signaling, acts as a pressor agent in blood pressure regulation, and is crucial for memory formation. CD47's interactions with SIRPA, SIRPG, and FAS/CD95 influence dendritic cell maturation, T-cell activation, apoptosis, and angiogenesis. As a monomer, CD47 intricately participates in diverse cellular and molecular pathways, contributing to physiological and pathological processes. CD47 Protein, Human (Biotinylated, HEK293, Fc-Avi) is the recombinant human-derived CD47 protein, expressed by HEK293, with C-Avi, C-hFc labeled tag. The total length of CD47 Protein, Human (Biotinylated, HEK293, Fc-Avi) is 121 a.a., with molecular weight of 55-66 kDa.

CD47, an adhesive protein, facilitates cell-to-cell interactions and serves as a receptor for thrombospondin THBS1, modulating integrin signaling through the activation of heterotrimeric G proteins. Involved in diverse cellular processes, CD47 contributes to signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation. Notably, it plays a role in modulating pulmonary endothelin EDN1 signaling and functions as a pressor agent in the regulation of blood pressure in response to THBS1. CD47 is crucial for memory formation and synaptic plasticity in the hippocampus, acting as a receptor for SIRPA and SIRPG, which impacts dendritic cell maturation, cytokine production, cell-cell adhesion, and T-cell activation. Furthermore, CD47 positively modulates FAS-dependent apoptosis in T-cells and suppresses angiogenesis, contributing to metabolic dysregulation during aging. In response to THBS1, CD47 negatively modulates wound healing, inhibits stem cell self-renewal, and may play a role in membrane transport and/or integrin-dependent signal transduction. As a monomer, CD47 interacts with THBS1, SIRPA, FAS/CD95, SIRPG, UBQLN1, UBQLN2, and potentially fibrinogen, highlighting its intricate involvement in cellular and molecular pathways.

Immobilized Human SIRP alpha His at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD47 with a linear range of <8 μg/mL.

Human

HEK293

C-Avi;C-hFc

Q08722 (Q19-P139)

961  [NCBI]

55-66 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized a 0.2 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.