CD73/5'-Nucleotidase Protein, Human (HEK293, His)

CD73/5'-Nucleotidase Protein hydrolyzes nucleotide monophosphates, favoring AMP as the primary substrate and showing a preference for ribonucleotide monophosphates. It releases inorganic phosphate and the corresponding nucleoside. CD73 also acts on other substrates, including IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD, and NMN. CD73/5'-Nucleotidase Protein, Human (HEK293, His) is the recombinant human-derived CD73/5'-Nucleotidase protein, expressed by HEK293 , with C-6*His labeled tag.

The CD73/5'-Nucleotidase protein assumes a crucial role in cellular processes by catalyzing the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside. Notably, AMP stands out as the preferred substrate for this enzyme, emphasizing its significance in the conversion of adenylate nucleotides. CD73/5'-Nucleotidase exhibits a preference for ribonucleotide monophosphates over their deoxyribose counterparts, showcasing its selectivity in substrate recognition. In addition to AMP, other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD, and NMN, further illustrating the versatility of CD73/5'-Nucleotidase in nucleotide metabolism and its role in regulating the balance of nucleotide pools within the cell.

1. 2 μg/mL (100 μL/well) of immobilized recombinant Human 5'-nucleotidase/CD73-His can bind Anti-Human CD73 mAb with an ED₅₀ value of 2.78 ng/mL.
2. Measured by its ability to hydrolyze the 5'-phosphate group from the substrate adenosine-5'-monophosphate (AMP). The specific activity is about 3×10⁵ pmol/min/μg.

Human

HEK293

C-6*His

P21589-1 (W27-K547)

4907  [NCBI]

Partial

WELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIK

Approximately 62.0 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 120 mM NaCl, 4 mM CaCl₂, 20% glycerol, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.