1. Recombinant Proteins
  2. CD73/5'-Nucleotidase Protein, Human (HEK 293, His)

CD73/5'-Nucleotidase Protein, Human (HEK 293, His)

Cat. No.: HY-P70010
Handling Instructions

For research use only. We do not sell to patients.

Protein Expression Service

Recombinant Proteins-MedChemExpress
Size Price Stock Quantity
10 μg $165 In-stock
Estimated Time of Arrival: December 31
50 μg $496 In-stock
Estimated Time of Arrival: December 31
100 μg   Get quote  
250 μg   Get quote  

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  • Technical Parameters

  • Properties

  • Documentation

Synonyms
rHu5'-nucleotidase/CD73, His; 5'-Nucleotidase; 5'-NT; Ecto-5'-Nucleotidase; CD73; NT5E; NT5; NTE
Species

Human

Source

HEK 293

Accession

P21589 (W27-K547)

Gene ID

4907

Molecular Weight

Approximately 62.0 kDa

AA Sequence

WELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIK

Purity

Greater than 95% as determined by reducing SDS-PAGE

Biological Activity
2 μg/mL (100 μL/well) of immobilized recombinant Human 5'-nucleotidase/CD73-His can bind Anti-Human CD73 mAb with an ED50 value of 7.8 ng/mL.
Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 120 mM NaCl, 4 mM CaCl2, 20% Glycerol, pH 7.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
CD73/5'-Nucleotidase Protein, Human (HEK 293, His)
Cat. No.:
HY-P70010
Quantity:
MCE Japan Authorized Agent: