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CD73/5'-Nucleotidase Protein, Human (HEK293, His)

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Based on 1 Customer Validation

CD73/5'-Nucleotidase Protein hydrolyzes nucleotide monophosphates, favoring AMP as the primary substrate and showing a preference for ribonucleotide monophosphates. It releases inorganic phosphate and the corresponding nucleoside. CD73 also acts on other substrates, including IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD, and NMN. CD73/5'-Nucleotidase Protein, Human (HEK293, His) is the recombinant human-derived CD73/5'-Nucleotidase protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for CD73/5'-Nucleotidase Protein, Human (HEK293, His)
  • CD73/5'-Nucleotidase Protein, Human (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

CD73/5'-Nucleotidase Protein hydrolyzes nucleotide monophosphates, favoring AMP as the primary substrate and showing a preference for ribonucleotide monophosphates. It releases inorganic phosphate and the corresponding nucleoside. CD73 also acts on other substrates, including IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD, and NMN. CD73/5'-Nucleotidase Protein, Human (HEK293, His) is the recombinant human-derived CD73/5'-Nucleotidase protein, expressed by HEK293 , with C-6*His labeled tag.

Background

The CD73/5'-Nucleotidase protein assumes a crucial role in cellular processes by catalyzing the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside. Notably, AMP stands out as the preferred substrate for this enzyme, emphasizing its significance in the conversion of adenylate nucleotides. CD73/5'-Nucleotidase exhibits a preference for ribonucleotide monophosphates over their deoxyribose counterparts, showcasing its selectivity in substrate recognition. In addition to AMP, other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD, and NMN, further illustrating the versatility of CD73/5'-Nucleotidase in nucleotide metabolism and its role in regulating the balance of nucleotide pools within the cell.

Verified Bioactivity

1. 2 μg/mL (100 μL/well) of immobilized recombinant Human 5'-nucleotidase/CD73-His can bind Anti-Human CD73 mAb with an ED50 value of 2.78 ng/mL.
2. Measured by its ability to hydrolyze the 5'-phosphate group from the substrate adenosine-5'-monophosphate (AMP). The specific activity is about 3×105 pmol/min/μg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P21589-1 (W27-K547)

  • Gene ID
    4907  [NCBI]
  • Molecular Construction
    • N-term
    • CD73 (W27-K547)
      Accession # P21589-1
    • 6*His
    • C-term
  • Protein Length

    Full Length of 5'-nucleotidase Chain

  • Synonyms

    NT5E; ENT; Prev. NT5; 5'-NT; IMP-Specific 5'-Nucleotidase; NTE; Thymidylate 5'-Phosphatase; 5'-Nucleotidase, Ecto (CD73); 5'-Deoxynucleotidase; Purine 5-Prime-Nucleotidase; Ecto-5'-Nucleotidase; 5' Nucleotidase (CD73); 5'-Nucleotidase; CD73 Antigen; CALJA; E5NT; CD73; NT; 5'-Nucleotidase Ecto; EN

  • AA Sequence

    WELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIK

  • Molecular Weight

    Approximately 62-68 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 120 mM NaCl, 4 mM CaCl2, 20% glycerol, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • CD73/5'-Nucleotidase Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for CD73/5'-Nucleotidase Protein, Human (HEK293, His)
  • CD73/5'-Nucleotidase Protein, Human (HEK293, His) Product Image