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  5. CD73/5'-Nucleotidase Protein, Human (521a.a, HEK293, His)

CD73/5'-Nucleotidase Protein, Human (521a.a, HEK293, His)

Cat. No.: HY-P70010
COA Handling Instructions

For research use only. We do not sell to patients.

Protein Expression Service

Size Price Stock Quantity
10 μg $140 In-stock
50 μg $400 In-stock
100 μg   Get quote  
250 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Biological Activity

1. 2 μg/mL (100 μL/well) of immobilized recombinant Human 5'-nucleotidase/CD73-His can bind Anti-Human CD73 mAb with an ED50 value of 2.78 ng/mL.
2. Measured by its ability to hydrolyze the 5'-phosphate group from the substrate adenosine-5'-monophosphate (AMP). The specific activity is about 3×105 pmol/min/μg.








P21589 (W27-K547)

Gene ID
rHu5'-nucleotidase/CD73, His; 5'-Nucleotidase; 5'-NT; Ecto-5'-Nucleotidase; CD73; NT5E; NT5; NTE
AA Sequence


Molecular Weight

Approximately 62.0 kDa

  • Greater than 95% as determined by reducing SDS-PAGE



Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 120 mM NaCl, 4 mM CaCl2, 20% Glycerol, pH 7.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.


Shipping with dry ice.

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD73/5'-Nucleotidase Protein, Human (521a.a, HEK293, His)
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