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CL-P1/COLEC12 Protein, Rat (sf9, His)

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Based on 1 Customer Validation

CL-P1/COLEC12 is a multifunctional scavenger receptor that effectively defends against microorganisms and promotes their binding and phagocytosis, covering Gram-positive and Gram-negative bacteria and yeast. In addition, it mediates the recognition, internalization, and degradation of oxidatively modified low-density lipoprotein (oxLDL) by vascular endothelial cells. CL-P1/COLEC12 Protein, Rat (sf9, His) is the recombinant rat-derived CL-P1/COLEC12 protein, expressed by Sf9 insect cells , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for CL-P1/COLEC12 Protein, Rat (sf9, His)
  • CL-P1/COLEC12 Protein, Rat (sf9, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

CL-P1/COLEC12 is a multifunctional scavenger receptor that effectively defends against microorganisms and promotes their binding and phagocytosis, covering Gram-positive and Gram-negative bacteria and yeast. In addition, it mediates the recognition, internalization, and degradation of oxidatively modified low-density lipoprotein (oxLDL) by vascular endothelial cells. CL-P1/COLEC12 Protein, Rat (sf9, His) is the recombinant rat-derived CL-P1/COLEC12 protein, expressed by Sf9 insect cells , with N-His labeled tag.

Background

CL-P1/COLEC12, a multifaceted scavenger receptor, plays a crucial role in host defense by promoting the binding and phagocytosis of various microbes, encompassing Gram-positive and Gram-negative bacteria as well as yeast. Additionally, it functions as a key mediator in the recognition, internalization, and degradation of oxidatively modified low-density lipoprotein (oxLDL) by vascular endothelial cells. The receptor exhibits versatile carbohydrate-binding capabilities, interacting with Gal-type ligands, D-galactose, L- and D-fucose, GalNAc, T, and Tn antigens in a calcium-dependent manner. Specifically, it internalizes GalNAc in nurse-like cells and binds to sialyl Lewis X, a trisaccharide, and asialo-orosomucoid (ASOR). Notably, the extracellular domain of CL-P1/COLEC12 forms a stable trimer and engages in interactions with fibrillar amyloid-beta peptide, suggesting its potential involvement in processes related to amyloid-beta pathology.

Verified Bioactivity

Immobilized Rat CL-P1 at 5 µg/mL (100 µL/well) can bind biotinylated advanced glycation endproducts of bovine serum albumin (AGE-BSA).The ED50 for this effect is 174.8 ng/mL.

MCE Validation Data

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Immobilized Rat CL-P1 at 5 µg/mL (100 µL/well) can bind biotinylated advanced glycation endproducts of bovine serum albumin (AGE-BSA).The ED50 for this effect is 174.8 ng/mL.

Technical Parameters

  • Species Rat
  • Source Sf9 insect cells
  • Tag N-His
  • Accession

    Q4V885 (A101-L742)

  • Gene ID
    361289  [NCBI]
  • Molecular Construction
    • N-term
    • His
    • CL-P1 (A101-L742)
      Accession # Q4V885
    • C-term
  • Protein Length

    Partial Extracellular Domain

  • Synonyms

    COLEC12; Collectin-12; Collectin Subfamily Member 12; HCL-P1; SCARA4; CLP1; SRCL; NSR2; CL-P1; Scavenger Receptor Class A, Member 4; Scavenger Receptor With C-Type Lectin; Scavenger Receptor Class A Member 4; Nurse Cell Scavenger Receptor 2; Collectin Sub

  • AA Sequence

    ALSTNSELSTFRSDILDLRQQLQEITEKTSKNKDMLEKLQANGDSLVDRQSQLKETLQNNSFLITTVNKTLQAYNGYVTNLQQDTSVLQGNLQSQMYSQNVVIMNLNNLNLTQVQQRNLITNLQRSVDDTSLAIQQIKNDFQNLQQVFLQAKKDTDWLKEKVQSLQTLAANNSALAKANNDTLEDMNNQLSSFTGQMDNITTISQANEQSMKDLQDLHKDTENRTAVKFSQLEERFQVFETDIVNIINNISYTAHHLRTLTSNLNDVRTTCTDTLTRHTDDLTSLNNTLVNIRLDSISLRMQQDMMRSRLDTEVANLSVVMEEMKLVDSKHGQLIKNFTILQGPPGPRGPKGDRGSQGPPGPTGNKGQKGEKGEPGPPGPAGERGTIGPVGPPGERGSKGSKGSQGPKGSRGSPGKPGPQGPSGDPGPPGPPGKDGLPGPQGPPGFQGLQGTVGEPGVPGPRGLPGLPGVPGMPGPKGPPGPPGPSGAMEPLALQNEPTPASEVNGCPPHWKNFTDKCYYFSVEKEIFEDAKLFCEDKSSHLVFINSREEQQWIKKQTMGRESHWIGLTDSEQESEWKWLDGTPVDYKNWKAGQPDNWGSGHGPGEDCAGLIYAGQWNDFQCDEINNFICEKEREAVPSSIL

  • Predicted Molecular Mass

    72.4 kDa

  • Molecular Weight

    Approximately 90 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 300 mM NaCl, 10% glycerol, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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    MedChemExpress Validation
     Immobilized Rat CL-P1 at 5 µg/mL (100 µL/well) can bind biotinylated advanced glycation endproducts of bovine serum albumin (AGE-BSA).The ED50 for this effect is 174.8 ng/mL.

  • CL-P1/COLEC12 Protein, Rat (sf9, His) Product Image
    1/16
  • Experimental Validation Results for CL-P1/COLEC12 Protein, Rat (sf9, His)
  • CL-P1/COLEC12 Protein, Rat (sf9, His) Product Image