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CLEC12A/MICL Protein, Cynomolgus (HEK293, His)

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CLEC12A/MICL Protein is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. CLEC12A is a cell surface receptor known to regulate signaling cascades and facilitate the tyrosine phosphorylation of target MAP kinases. It interacts with PTPN6 and PTPN11, indicating its involvement in intricate cellular signaling processes. CLEC12A/MICL Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived CLEC12A/MICL protein, expressed by HEK293 , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Cynomolgus
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • CLEC12A/MICL Protein, Cynomolgus (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

CLEC12A/MICL Protein is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. CLEC12A is a cell surface receptor known to regulate signaling cascades and facilitate the tyrosine phosphorylation of target MAP kinases. It interacts with PTPN6 and PTPN11, indicating its involvement in intricate cellular signaling processes. CLEC12A/MICL Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived CLEC12A/MICL protein, expressed by HEK293 , with N-His labeled tag.

Background

C-type lectin domain family 12 member A (CLEC12A) is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. CLEC12A is a type II transmembrane protein expressed in humans by myeloid cells, including neutrophils, monocytes, plasmacytoid BDCA2+, and myeloid CD1+c/CD19- dendritic cells. CLEC12A is a cell surface receptor known to regulate signaling cascades and facilitate the tyrosine phosphorylation of target MAP kinases. It interacts with PTPN6 and PTPN11, indicating its involvement in intricate cellular signaling processes. In addition, CLEC12A may regulate the inflammatory response to infectious agents through direct interaction with pathogen-derived crystals or antigens. It regulates cytokine synthesis by synergizing or antagonizing signaling pathways of a variety of receptors including TLRs and the CD40 receptor in a stimulus-dependent manner. CLEC12A is also involved in adaptive immunity through its ability to internalize antigen for routing to Major Histocompatibility complex (MHC) compartments and presentation to T cells[1][2].

Technical Parameters

  • Species Cynomolgus
  • Source HEK293
  • Tag N-8*His
  • Accession

    A0A2K5WXQ6 (H32-A232)

  • Gene ID
    102143884  [NCBI]
  • Molecular Construction
    • N-term
    • 8*His
    • CLEC12A (H32-A232)
      Accession # A0A2K5WXQ6
    • C-term
  • Protein Length

    Partial

  • Synonyms

    CLEC12A; CLL1; C-Type Lectin Domain Family 12 Member A; C-Type Lectin Domain Family 12, Member A; DCAL-2; C-Type Lectin-Like Molecule-1; CLL-1; C-Type Lectin-Like Molecule 1; MICL; C-Type Lectin Protein CLL-1; Myeloid Inhibitory C-Type Lectin-Like Recepto

  • AA Sequence

    HITLKTAMKKMNKLQNINEELQRNVSLQLMSNMNSSNKIRNLSTTLQTIATRLCRELYSKEQEHKCKPCPRRWIWHKDSCYFLSDDVRTWQESRMACAAQNASLLKINNKNALEFIKSQSTSYPYWLGLSPEKDYSYGTSVDDIINSSAWVTRNASDLNNMFCGYINRIYVHYDYCIYRKKMICEKMANPVQLGFIHFREA

  • Predicted Molecular Mass

    24.6 kDa

  • Molecular Weight

    Approximately 38-50 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • CLEC12A/MICL Protein, Cynomolgus (HEK293, His) Product Image
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  • CLEC12A/MICL Protein, Cynomolgus (HEK293, His) Product Image