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  3. Pattern Recognition Receptors
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  5. DNGR-1/CLEC9A
  6. CLEC9A Protein, Human (HEK293, Fc)

CLEC9A Protein, Human (HEK293, Fc)

Cat. No.: HY-P77904
COA Handling Instructions

The CLEC9A Protein, expressed on a specific subset of myeloid cells, efficiently uptakes and processes material from deceased cells. It recognizes filamentous actin, especially with actin-binding domains like spectrin, when cell membranes are compromised. This facilitates Syk-dependent cross-presentation of antigens from dead cells. CLEC9A functions as a homodimer, highlighting its role in specialized endocytic processes of myeloid cells. CLEC9A Protein, Human (HEK293, Fc) is the recombinant human-derived CLEC9A protein, expressed by HEK293 , with N-hFc labeled tag. The total length of CLEC9A Protein, Human (HEK293, Fc) is 185 a.a., with molecular weight of 55-65 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

The CLEC9A Protein, expressed on a specific subset of myeloid cells, efficiently uptakes and processes material from deceased cells. It recognizes filamentous actin, especially with actin-binding domains like spectrin, when cell membranes are compromised. This facilitates Syk-dependent cross-presentation of antigens from dead cells. CLEC9A functions as a homodimer, highlighting its role in specialized endocytic processes of myeloid cells. CLEC9A Protein, Human (HEK293, Fc) is the recombinant human-derived CLEC9A protein, expressed by HEK293 , with N-hFc labeled tag. The total length of CLEC9A Protein, Human (HEK293, Fc) is 185 a.a., with molecular weight of 55-65 kDa.

Background

CLEC9A Protein operates as an endocytic receptor uniquely expressed on a specific subset of myeloid cells, designed for the efficient uptake and processing of material derived from deceased cells. This receptor exhibits a distinct recognition capability for the filamentous form of actin, particularly in conjunction with specific actin-binding domains found in cytoskeletal proteins like spectrin. This recognition occurs when cell membranes are compromised, facilitating the cross-presentation of antigens associated with dead cells in a Syk-dependent manner. Notably, CLEC9A functions as a homodimer, emphasizing its role in the specialized endocytic processes performed by this subset of myeloid cells.

Biological Activity

Immobilized Human CLEC9A, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CLEC9A Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.

Species

Human

Source

HEK293

Tag

N-hFc

Accession

Q6UXN8 (K57-V241)

Gene ID
Molecular Construction
N-term
hFc
CLEC9A (K57-V241)
Accession # Q6UXN8
C-term
Synonyms
CD370; CLEC9A; 9830005G06Rik; DNGR-1; HEEE9341; UNQ9341
Molecular Weight

55-65 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

CLEC9A Protein, Human (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
CLEC9A Protein, Human (HEK293, Fc)
Cat. No.:
HY-P77904
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