CLEC9A Protein, Human (HEK293, Fc)
Based on 1 Customer Validation
The CLEC9A Protein, expressed on a specific subset of myeloid cells, efficiently uptakes and processes material from deceased cells. It recognizes filamentous actin, especially with actin-binding domains like spectrin, when cell membranes are compromised. This facilitates Syk-dependent cross-presentation of antigens from dead cells. CLEC9A functions as a homodimer, highlighting its role in specialized endocytic processes of myeloid cells. CLEC9A Protein, Human (HEK293, Fc) is the recombinant human-derived CLEC9A protein, expressed by HEK293 , with N-hFc labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The CLEC9A Protein, expressed on a specific subset of myeloid cells, efficiently uptakes and processes material from deceased cells. It recognizes filamentous actin, especially with actin-binding domains like spectrin, when cell membranes are compromised. This facilitates Syk-dependent cross-presentation of antigens from dead cells. CLEC9A functions as a homodimer, highlighting its role in specialized endocytic processes of myeloid cells. CLEC9A Protein, Human (HEK293, Fc) is the recombinant human-derived CLEC9A protein, expressed by HEK293 , with N-hFc labeled tag.
Background
CLEC9A Protein operates as an endocytic receptor uniquely expressed on a specific subset of myeloid cells, designed for the efficient uptake and processing of material derived from deceased cells. This receptor exhibits a distinct recognition capability for the filamentous form of actin, particularly in conjunction with specific actin-binding domains found in cytoskeletal proteins like spectrin. This recognition occurs when cell membranes are compromised, facilitating the cross-presentation of antigens associated with dead cells in a Syk-dependent manner. Notably, CLEC9A functions as a homodimer, emphasizing its role in the specialized endocytic processes performed by this subset of myeloid cells.
Verified Bioactivity
Immobilized Human CLEC9A, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CLEC9A Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.
Technical Parameters
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Species Human
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Source HEK293
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Tag N-hFc
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Accession
Q6UXN8 (K57-V241)
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Molecular Construction
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N-term
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hFc
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CLEC9A (K57-V241)
Accession # Q6UXN8 -
C-term
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Protein Length
Extracellular Domain
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Synonyms
CLEC9A; CD370; C-Type Lectin Domain Containing 9A; Dendritic Cell Natural Killer Lectin Group Receptor 1; C-Type Lectin Domain Family 9 Member A; DNGR1; HEEE9341; C-Type Lectin Domain Family 9, Member A; UNQ9341; CD370 Antigen; DNGR-1
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AA Sequence
KLLQVSTIAMQQQEKLIQQERALLNFTEWKRSCALQMKYCQAFMQNSLSSAHNSSPCPNNWIQNRESCYYVSEIWSIWHTSQENCLKEGSTLLQIESKEEMDFITGSLRKIKGSYDYWVGLSQDGHSGRWLWQDGSSPSPGLLPAERSQSANQVCGYVKSNSLLSSNCSTWKYFICEKYALRSSV
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Predicted Molecular Mass
48.5 kDa
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Molecular Weight
Approximately 55-65 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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