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Coagulation factor XIII A/F13A Protein, Human (HEK293, His)

Customer Review

Based on 1 Customer Validation

Coagulation factor XIII A chain is the A chain of factor XIII. As a coagulation factor, Factor XIII A chain alters clot stabilization and bleeding. Factor XIII A chain regulates multiple aspects of wound healing: stabilizes fibrin clots and extracellular matrix, modulates monocyte functions, promote angiogenesis, and upregulates proangiogenic early growth response transcription factor 1.
Coagulation factor XIII A/F13A Protein, Human (HEK293, His) is the recombinant human-derived Coagulation factor XIII A/F13A protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for Coagulation factor XIII A/F13A Protein, Human (HEK293, His)
  • Coagulation factor XIII A/F13A Protein, Human (HEK293, His) Product Image
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609-228-6898
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Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Coagulation factor XIII A chain is the A chain of factor XIII. As a coagulation factor, Factor XIII A chain alters clot stabilization and bleeding. Factor XIII A chain regulates multiple aspects of wound healing: stabilizes fibrin clots and extracellular matrix, modulates monocyte functions, promote angiogenesis, and upregulates proangiogenic early growth response transcription factor 1[1][2][3][4].
Coagulation factor XIII A/F13A Protein, Human (HEK293, His) is the recombinant human-derived Coagulation factor XIII A/F13A protein, expressed by HEK293 , with C-6*His labeled tag.

Background

Coagulation factor XIII is critical in the coagulation cascade chain, and enhances the stability of blood clot formation. Factor XIII is involved in trauma, wound healing, tissue repair, pregnancy, and even bone metabolism. Factor XIII can bind to αvβ3 integrin, inducing VEGFR-2 activation, promoting endothelial cell proliferation and survival, and angiogenesis[1].
Factor XIII, a transglutaminase, is a complex of two A (gene F13A1) chains with catalytic activity and two B chains (gene F13B)[2]. Factor XIII A chain can be activated by thrombin and Ca2+. Factor XIII A usually exists in the cytoplasm of certain cells, particularly platelets and monocytes/macrophages[3].
Factor XIII A chain plays a role in wound healing, inflammatory and malignant diseases[4].

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    AAH27963.1 (G39-M732)

  • Gene ID
    2162  [NCBI]
  • Molecular Construction
    • N-term
    • F13A (G39-M732)
      Accession # AAH27963.1
    • 6*His
    • C-term
  • Protein Length

    Full Length of Coagulation factor XIII A chain Chain

  • Synonyms

    F13A1; Coagulation Factor XIII, A Polypeptide; Prev. F13A; Transglutaminase. Plasma; Protein-Glutamine Gamma-Glutamyltransferase A Chain; Coagulation Factor XIII; Coagulation Factor XIII, A1 Polypeptide; FSF, A Subunit; Coagulation Factor XIIIa; Fibrinoli

  • AA Sequence

    GVNLQEFLNVTSVHLFKERWDTNKVDHHTDKYENNKLIVRRGQSFYVQIDFSRPYDPRRDLFRVEYVIGRYPQENKGTYIPVPIVSELQSGKWGAKIVMREDRSVRLSIQSSPKCIVGKFRMYVAVWTPYGVLRTSRNPETDTYILFNPWCEDDAVYLDNEKEREEYVLNDIGVIFYGEVNDIKTRSWSYGQFEDGILDTCLYVMDRAQMDLSGRGNPIKVSRVGSAMVNAKDDEGVLVGSWDNIYAYGVPPSAWTGSVDILLEYRSSENPVRYGQCWVFAGVFNTFLRCLGIPARIVTNYFSAHDNDANLQMDIFLEEDGNVNSKLTKDSVWNYHCWNEAWMTRPDLPVGFGGWQAVDSTPQENSDGMYRCGPASVQAIKHGHVCFQFDAPFVFAEVNSDLIYITAKKDGTHVVENVDATHIGKLIVTKQIGGDGMMDITDTYKFQEGQEEERLALETALMYGAKKPLNTEGVMKSRSNVDMDFEVENAVLGKDFKLSITFRNNSHNRYTITAYLSANITFYTGVPKAEFKKETFDVTLEPLSFKKEAVLIQAGEYMGQLLEQASLHFFVTARINETRDVLAKQKSTVLTIPEIIIKVRGTQVVGSDMTVIVEFTNPLKETLRNVWVHLDGPGVTRPMKKMFREIRPNSTVQWEEVCRPWVSGHRKLIASMSSDSLRHVYGELDVQIQRRPSM

  • Predicted Molecular Mass

    80.3 kDa

  • Molecular Weight

    Approximately 80-90 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

1.Supplied as a 0.22 μm filtered solution of 50 mM NaCl, 5% sucrose, 0.3% histidine , pH 8.0.
2.Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 5% sucrose, 10% glycerol, 0.02% Tween 80, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Coagulation factor XIII A/F13A Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Coagulation factor XIII A/F13A Protein, Human (HEK293, His)
  • Coagulation factor XIII A/F13A Protein, Human (HEK293, His) Product Image