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CRELD2 Protein, Human (329a.a, HEK293, His)

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Based on 1 Customer Validation

CRELD2 Protein, potentially acting as a protein disulfide isomerase, may play a role in the unfolded protein response. It is also implicated in regulating the transport of the alpha4-beta2 neuronal acetylcholine receptor, showcasing its versatility in cellular processes related to protein folding and neuronal receptor dynamics. These functions suggest CRELD2's involvement in maintaining cellular homeostasis. CRELD2 Protein, Human (329a.a, HEK293, His) is the recombinant human-derived CRELD2 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CRELD2 Protein, Human (329a.a, HEK293, His)
  • CRELD2 Protein, Human (329a.a, HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

CRELD2 Protein, potentially acting as a protein disulfide isomerase, may play a role in the unfolded protein response. It is also implicated in regulating the transport of the alpha4-beta2 neuronal acetylcholine receptor, showcasing its versatility in cellular processes related to protein folding and neuronal receptor dynamics. These functions suggest CRELD2's involvement in maintaining cellular homeostasis. CRELD2 Protein, Human (329a.a, HEK293, His) is the recombinant human-derived CRELD2 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

CRELD2 protein, likely functioning as a protein disulfide isomerase, may contribute to the intricate processes associated with the unfolded protein response. Additionally, there is a potential role for CRELD2 in the regulation of the transport of the alpha4-beta2 neuronal acetylcholine receptor. These functions highlight the versatility of CRELD2 in cellular processes related to protein folding and neuronal receptor dynamics, suggesting its involvement in maintaining cellular homeostasis.

Verified Bioactivity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is typically 0.7722 μg/mL.

MCE Validation Data

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is typically 0.7722 μg/mL, corresponding to a specific activity is 1.295×103 units/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q6UXH1-1 (A25-L353)

  • Gene ID
    79174  [NCBI]
  • Molecular Construction
    • N-term
    • CRELD2 (A25-L353)
      Accession # Q6UXH1-1
    • 6*His
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    CRELD2; Cysteine-Rich With EGF-Like Domain Protein 2; CRELD Disulfide Isomerase 2; Protein Disulfide Isomerase CRELD2; Cysteine Rich With EGF Like Domains 2; MGC11256

  • AA Sequence

    AKKPTPCHRCRGLVDKFNQGMVDTAKKNFGGGNTAWEEKTLSKYESSEIRLLEILEGLCESSDFECNQMLEAQEEHLEAWWLQLKSEYPDLFEWFCVKTLKVCCSPGTYGPDCLACQGGSQRPCSGNGHCSGDGSRQGDGSCRCHMGYQGPLCTDCMDGYFSSLRNETHSICTACDESCKTCSGLTNRDCGECEVGWVLDEGACVDVDECAAEPPPCSAAQFCKNANGSYTCEECDSSCVGCTGEGPGNCKECISGYAREHGQCADVDECSLAEKTCVRKNENCYNTPGSYVCVCPDGFEETEDACVPPAEAEATEGESPTQLPSREDL

  • Predicted Molecular Mass

    36.4 kDa

  • Molecular Weight

    Approximately 42 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Ortiz JA, et al. The cysteine-rich with EGF-like domains 2 (CRELD2) protein interacts with the large cytoplasmic domain of human neuronal nicotinic acetylcholine receptor alpha4 and beta2 subunits. J Neurochem. 2005 Dec;95(6):1585-96. [Content Brief]

[2]. Tang Q, et al. CRELD2, endoplasmic reticulum stress, and human diseases. Front Endocrinol (Lausanne). 2023 Mar 2;14:1117414. [Content Brief]

[3]. Maslen CL, et al. CRELD2: gene mapping, alternate splicing, and comparative genomic identification of the promoter region. Gene. 2006 Nov 1;382:111-20 [Content Brief]

[4]. Boyle ST, et al. ROCK-mediated selective activation of PERK signalling causes fibroblast reprogramming and tumour progression through a CRELD2-dependent mechanism. Nat Cell Biol. 2020 Jul;22(7):882-895. [Content Brief]

[5]. Dennis EP, et al. CRELD2 Is a Novel LRP1 Chaperone That Regulates Noncanonical WNT Signaling in Skeletal Development. J Bone Miner Res. 2020 Aug;35(8):1452-1469. [Content Brief]

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Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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    MedChemExpress Validation
     Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is typically 0.7722 μg/mL, corresponding to a specific activity is 1.295×103 units/mg.

  • CRELD2 Protein, Human (329a.a, HEK293, His) Product Image
    1/16
  • Experimental Validation Results for CRELD2 Protein, Human (329a.a, HEK293, His)
  • CRELD2 Protein, Human (329a.a, HEK293, His) Product Image