Casein Kinase I gamma-2/CSNK1G2 Protein, Human (Active, sf9, GST)
Casein kinase I gamma-2 is a serine/threonine protein kinase that targets acidic proteins and phosphorylates a variety of substrates, such as COL4A3BP/CERT, MTA1 and SMAD3, especially destabilizing SMAD3 and inhibiting TGF- beta reaction. Hyperphosphorylation of COL4A3BP/CERT disrupts ER-to-Golgi transport and affects the synthesis of ceramide and sphingomyelin. Casein Kinase I gamma-2/CSNK1G2 Protein, Human (sf9, GST) is the recombinant human-derived CSNK1G2, expressed by Sf9 insect cells, with GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Casein kinase I gamma-2 is a serine/threonine protein kinase that targets acidic proteins and phosphorylates a variety of substrates, such as COL4A3BP/CERT, MTA1 and SMAD3, especially destabilizing SMAD3 and inhibiting TGF- beta reaction. Hyperphosphorylation of COL4A3BP/CERT disrupts ER-to-Golgi transport and affects the synthesis of ceramide and sphingomyelin. Casein Kinase I gamma-2/CSNK1G2 Protein, Human (sf9, GST) is the recombinant human-derived CSNK1G2, expressed by Sf9 insect cells, with GST labeled tag.
Background
Casein Kinase I gamma-2, a serine/threonine-protein kinase, exhibits a preference for acidic proteins, with caseins being prominent substrates. Demonstrating versatility, it phosphorylates a diverse array of proteins and actively participates in Wnt signaling. Noteworthy targets include COL4A3BP/CERT, MTA1, and SMAD3. Phosphorylation of SMAD3 serves to destabilize the protein, leading to ligand-dependent ubiquitination and subsequent proteasome degradation, thereby inhibiting TGF-beta responses. Hyperphosphorylation of the serine-repeat motif in COL4A3BP/CERT results in its inactivation, disrupting ER-to-Golgi transport and suppressing ceramide and sphingomyelin synthesis. Additionally, Casein Kinase I gamma-2 plays crucial roles in brain development, vesicular trafficking, neurotransmitter release from synaptic vesicles, and the regulation of fast synaptic transmission mediated by glutamate. Moreover, it contributes to the control of reactive oxygen species (ROS) levels, further emphasizing its multifaceted involvement in cellular processes.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag GST
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Accession
P78368 (D2-K415)
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Gene ID1455
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Protein Length
Full Length
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Synonyms
CSNK1G2; Non-Specific Serine/Threonine Protein Kinase; Casein Kinase 1 Gamma 2; Casein Kinase 1, Gamma 2; CK1g2; Casein Kinase I; Casein Kinase I Isoform Gamma-2; CK1G2; CKI-Gamma 2
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AA Sequence
DFDKKGGKGETEEGRRMSKAGGGRSSHGIRSSGTSSGVLMVGPNFRVGKKIGCGNFGELRLGKNLYTNEYVAIKLEPIKSRAPQLHLEYRFYKQLSATEGVPQVYYFGPCGKYNAMVLELLGPSLEDLFDLCDRTFTLKTVLMIAIQLITRMEYVHTKSLIYRDVKPENFLVGRPGTKRQHAIHIIDFGLAKEYIDPETKKHIPYREHKSLTGTARYMSINTHLGKEQSRRDDLEALGHMFMYFLRGSLPWQGLKADTLKERYQKIGDTKRATPIEVLCENFPEEMATYLRYVRRLDFFEKPDYDYLRKLFTDLFDRSGFVFDYEYDWAGKPLPTPIGTVHTDLPSQPQLRDKTQPHSKNQALNSTNGELNADDPTAGHSNAPITAPAEVEVADETKCCCFFKRRKRKSLQRHK
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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