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D-amino-acid oxidase/DAO Protein, Human (His)

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Based on 1 Customer Validation

D-amino-acid oxidase Protein, Human (His) is a recombinant D-amino-acid oxidase protein with a His tag. D-amino acid oxidase (DAO) is a flavin adenine dinucleotide (FAD)-dependent oxidase metabolizing neutral and polar D-amino acids.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for D-amino-acid oxidase/DAO Protein, Human (His)
  • D-amino-acid oxidase/DAO Protein, Human (His) Product Image
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609-228-6898
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

D-amino-acid oxidase Protein, Human (His) is a recombinant D-amino-acid oxidase protein with a His tag. D-amino acid oxidase (DAO) is a flavin adenine dinucleotide (FAD)-dependent oxidase metabolizing neutral and polar D-amino acids[1].

Background

D-amino acid oxidase (DAO) is a flavin adenine dinucleotide (FAD)–dependent peroxisomal enzyme that catalyzes the oxidation of neutral and polar D-amino acids with a strict stereospecificity to give α-keto acids, ammonia, and reactive oxygen species (ROS)[1].

Verified Bioactivity

Measured by its ability to produce hydrogen peroxide during the oxidative deamination of D-amino acids that incubate the reactions for 10 minutes at 37 °C. The specific activity is > 1000 pmol/min/μg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-6*His
  • Accession

    P14920 (M1-L347)

  • Gene ID
    1610  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His
    • DAO (M1-L347)
      Accession # P14920
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    DAO; DAAO; D-Amino Acid Oxidase; D-Amino-Acid Oxidase; DAMOX; OXDA

  • AA Sequence

    MRVVVIGAGVIGLSTALCIHERYHSVLQPLDIKVYADRFTPLTTTDVAAGLWQPYLSDPNNPQEADWSQQTFDYLLSHVHSPNAENLGLFLISGYNLFHEAIPDPSWKDTVLGFRKLTPRELDMFPDYGYGWFHTSLILEGKNYLQWLTERLTERGVKFFQRKVESFEEVAREGADVIVNCTGVWAGALQRDPLLQPGRGQIMKVDAPWMKHFILTHDPERGIYNSPYIIPGTQTVTLGGIFQLGNWSELNNIQDHNTIWEGCCRLEPTLKNARIIGERTGFRPVRPQIRLEREQLRTGPSNTEVIHNYGHGGYGLTIHWGCALEAAKLFGRILEEKKLSRMPPSHL

  • Predicted Molecular Mass

    41.6 kDa

  • Molecular Weight

    Approximately 40 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 20% Glycerol, 1 mM DTT, 2 mM EDTA, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

References

[1]. Nagano T, et al. d-amino acid oxidase promotes cellular senescence via the production of reactive oxygen species. Life Sci Alliance. 2019;2(1):e201800045. Published 2019 Jan 18. [Content Brief]

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • D-amino-acid oxidase/DAO Protein, Human (His) Product Image
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  • Experimental Validation Results for D-amino-acid oxidase/DAO Protein, Human (His)
  • D-amino-acid oxidase/DAO Protein, Human (His) Product Image