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DKK-3 Protein, Human (R335G, HEK293, His)

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Based on 1 Customer Validation

DKK-3 protein antagonizes canonical Wnt signaling by blocking LRP5/6-Wnt interactions and forming a ternary complex with KREMEN, thereby promoting LRP5/6 internalization.In vertebrate development, DKK-3, together with other DKKs, critically inhibits Wnt-regulated processes such as axial patterning, limb development, somitogenesis, and eye formation.DKK-3 Protein, Human (R335G, HEK293, His) is the recombinant human-derived DKK-3 protein, expressed by HEK293 , with C-6*His labeled tag and R335G, , , , mutation.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for DKK-3 Protein, Human (R335G, HEK293, His)
  • Experimental Validation Results for DKK-3 Protein, Human (R335G, HEK293, His)
  • DKK-3 Protein, Human (R335G, HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

DKK-3 protein antagonizes canonical Wnt signaling by blocking LRP5/6-Wnt interactions and forming a ternary complex with KREMEN, thereby promoting LRP5/6 internalization.In vertebrate development, DKK-3, together with other DKKs, critically inhibits Wnt-regulated processes such as axial patterning, limb development, somitogenesis, and eye formation.DKK-3 Protein, Human (R335G, HEK293, His) is the recombinant human-derived DKK-3 protein, expressed by HEK293 , with C-6*His labeled tag and R335G, , , , mutation.

Background

The DKK-3 protein functions as an antagonist of canonical Wnt signaling by impeding the interaction between LRP5/6 and Wnt and forming a ternary complex with the transmembrane protein KREMEN, facilitating the internalization of LRP5/6. Within vertebrate development, DKKs, including DKK-3, play a crucial role by locally inhibiting Wnt-regulated processes such as antero-posterior axial patterning, limb development, somitogenesis, and eye formation. In the adult, DKKs are implicated in diverse physiological and pathological conditions, encompassing bone formation, bone disease, cancer, and Alzheimer's disease (By similarity). Notably, DKK-3's interaction with LRP5 and LRP6 highlights its regulatory involvement in inhibiting Wnt signaling and its potential impact on various cellular processes.

Verified Bioactivity

Measured by its ability to inhibit the proliferation of Hela human cervical carcinoma cell. The ED50 for this effect is 3.621 μg/mL, corresponding to a specific activity is 276.167 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to inhibit the proliferation of Hela human cervical carcinoma cell. The ED50 for this effect is 3.621 μg/mL, corresponding to a specific activity is 276.167 units/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q9UBP4 (A22-I350, R335G)

  • Gene ID
    27122  [NCBI]
  • Molecular Construction
    • N-term
    • DKK-3 (A22-I350, R335G)
      Accession # Q9UBP4
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    DKK3; HDkk-3; Dickkopf Wnt Signaling Pathway Inhibitor 3; Dkk-3; REIC; Dickkopf WNT Signaling Pathway Inhibitor 3; Dickkopf-Related Protein 3; Dickkopf (Xenopus Laevis) Homolog 3; CRRL; Dickkopf 3 Homolog (Xenopus Laevis); RIG; Dickkopf 3 Homolog; Cardiom

  • AA Sequence

    APAPTATSAPVKPGPALSYPQEEATLNEMFREVEELMEDTQHKLRSAVEEMEAEEAAAKASSEVNLANLPPSYHNETNTDTKVGNNTIHVHREIHKITNNQTGQMVFSETVITSVGDEEGRRSHECIIDEDCGPSMYCQFASFQYTCQPCRGQRMLCTRDSECCGDQLCVWGHCTKMATRGSNGTICDNQRDCQPGLCCAFQRGLLFPVCTPLPVEGELCHDPASRLLDLITWELEPDGALDRCPCASGLLCQPHSHSLVYVCKPTFVGSRDQDGEILLPREVPDEYEVGSFMEEVRQELEDLERSLTEEMALGEPAAAAAALLGGEEI

  • Molecular Weight

    Approximately 50-65 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit the proliferation of Hela human cervical carcinoma cell. The ED50 for this effect is 3.621 μg/mL, corresponding to a specific activity is 276.167 units/mg.

  • DKK-3 Protein, Human (R335G, HEK293, His) Product Image
    1/16
  • Experimental Validation Results for DKK-3 Protein, Human (R335G, HEK293, His)
  • Experimental Validation Results for DKK-3 Protein, Human (R335G, HEK293, His)
  • DKK-3 Protein, Human (R335G, HEK293, His) Product Image