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DKK-3 Protein, Human (R335G, HEK293, His)

Cat. No.: HY-P78681
COA Handling Instructions

DKK-3 protein antagonizes canonical Wnt signaling by blocking LRP5/6-Wnt interaction and forming a ternary complex with KREMEN, promoting LRP5/6 internalization. In vertebrate development, DKK-3, along with other DKKs, crucially inhibits Wnt-regulated processes like axial patterning, limb development, somitogenesis, and eye formation. In adults, DKKs, including DKK-3, are implicated in diverse conditions like bone formation, bone disease, cancer, and Alzheimer's. DKK-3's interaction with LRP5 and LRP6 underscores its regulatory role in inhibiting Wnt signaling and its potential impact on various cellular processes. DKK-3 Protein, Human (R335G, HEK293, His) is the recombinant human-derived DKK-3 protein, expressed by HEK293 , with C-6*His labeled tag and R335G mutation. The total length of DKK-3 Protein, Human (R335G, HEK293, His) is 329 a.a., with molecular weight of 50-66 kDa.

For research use only. We do not sell to patients.

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Description

DKK-3 protein antagonizes canonical Wnt signaling by blocking LRP5/6-Wnt interaction and forming a ternary complex with KREMEN, promoting LRP5/6 internalization. In vertebrate development, DKK-3, along with other DKKs, crucially inhibits Wnt-regulated processes like axial patterning, limb development, somitogenesis, and eye formation. In adults, DKKs, including DKK-3, are implicated in diverse conditions like bone formation, bone disease, cancer, and Alzheimer's. DKK-3's interaction with LRP5 and LRP6 underscores its regulatory role in inhibiting Wnt signaling and its potential impact on various cellular processes. DKK-3 Protein, Human (R335G, HEK293, His) is the recombinant human-derived DKK-3 protein, expressed by HEK293 , with C-6*His labeled tag and R335G mutation. The total length of DKK-3 Protein, Human (R335G, HEK293, His) is 329 a.a., with molecular weight of 50-66 kDa.

Background

The DKK-3 protein functions as an antagonist of canonical Wnt signaling by impeding the interaction between LRP5/6 and Wnt and forming a ternary complex with the transmembrane protein KREMEN, facilitating the internalization of LRP5/6. Within vertebrate development, DKKs, including DKK-3, play a crucial role by locally inhibiting Wnt-regulated processes such as antero-posterior axial patterning, limb development, somitogenesis, and eye formation. In the adult, DKKs are implicated in diverse physiological and pathological conditions, encompassing bone formation, bone disease, cancer, and Alzheimer's disease (By similarity). Notably, DKK-3's interaction with LRP5 and LRP6 highlights its regulatory involvement in inhibiting Wnt signaling and its potential impact on various cellular processes.

Biological Activity

Measured by its ability to inhibit the proliferation of Hela human cervical carcinoma cell. The ED50 for this effect is 3.621 μg/mL, corresponding to a specific activity is 276.167 units/mg.

  • Measured by its ability to inhibit the proliferation of Hela human cervical carcinoma cell. The ED50 for this effect is 3.621 μg/mL, corresponding to a specific activity is 276.167 units/mg.
Species

Human

Source

HEK293

Tag

C-6*His

Accession

Q9UBP4-1 (A22-I350, R335G)

Gene ID
Molecular Construction
N-term
DKK-3 (A22-I350, R335G)
Accession # Q9UBP4-1
6*His
C-term
Synonyms
DKK3; REIC; RIG
AA Sequence

APAPTATSAPVKPGPALSYPQEEATLNEMFREVEELMEDTQHKLRSAVEEMEAEEAAAKASSEVNLANLPPSYHNETNTDTKVGNNTIHVHREIHKITNNQTGQMVFSETVITSVGDEEGRRSHECIIDEDCGPSMYCQFASFQYTCQPCRGQRMLCTRDSECCGDQLCVWGHCTKMATRGSNGTICDNQRDCQPGLCCAFQRGLLFPVCTPLPVEGELCHDPASRLLDLITWELEPDGALDRCPCASGLLCQPHSHSLVYVCKPTFVGSRDQDGEILLPREVPDEYEVGSFMEEVRQELEDLERSLTEEMALGEPAAAAAALLGGEEI

Molecular Weight

50-66 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

DKK-3 Protein, Human (R335G, HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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DKK-3 Protein, Human (R335G, HEK293, His)
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