ECE-2 Protein, Human (I320, HEK293, hFc)
The ECE-2 protein plays a crucial role in vascular regulation by catalyzing the conversion of large endothelin-1 to endothelin-1, helping to regulate blood pressure and vascular tone. Furthermore, ECE-2 exhibits potential methyltransferase activity and may be involved in amyloid-β processing, suggesting that this enzyme has a broader impact on cellular function than its established role in endothelin processing. ECE-2 Protein, Human (HEK293, hFc) is the recombinant human-derived ECE-2 protein, expressed by HEK293 , with N-hFc labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The ECE-2 protein plays a crucial role in vascular regulation by catalyzing the conversion of large endothelin-1 to endothelin-1, helping to regulate blood pressure and vascular tone. Furthermore, ECE-2 exhibits potential methyltransferase activity and may be involved in amyloid-β processing, suggesting that this enzyme has a broader impact on cellular function than its established role in endothelin processing. ECE-2 Protein, Human (HEK293, hFc) is the recombinant human-derived ECE-2 protein, expressed by HEK293 , with N-hFc labeled tag.
Background
The ECE-2 protein is an essential enzyme with dual functionality. Primarily, it plays a crucial role in converting big endothelin-1 to endothelin-1, a biologically active peptide involved in the regulation of vascular tone and blood pressure. In addition to its role in the endothelin system, ECE-2 exhibits potential methyltransferase activity, implying its involvement in the transfer of methyl groups between molecules. Moreover, there is a suggested role for ECE-2 in amyloid-beta processing, indicating a possible association with processes related to neurodegenerative conditions. The multifaceted nature of ECE-2's activities underscores its significance in both cardiovascular physiology, through endothelin processing, and potential contributions to molecular events associated with neurodegenerative disorders (
Technical Parameters
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Species Human
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Source HEK293
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Tag N-hFc
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Accession
P0DPD8-1 (G200-W883,I320)
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Gene ID110599583 [NCBI]
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Molecular Construction
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N-term
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hFc
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ECE-2 (G100-W883,I320)
Accession # P0DPD8-1 -
C-term
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Protein Length
Full Length of Lumenal Chain
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Synonyms
EEF1AKMT4-ECE2; EEF1AKMT4-ECE2 Readthrough Transcript Protein; EEF1AKMT4-ECE2 Readthrough
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AA Sequence
GVQYHRDPSHSTCLTEACIRVAGKILESLDRGVSPCEDFYQFSCGGWIRRNPLPDGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAEQKTQRFYLSCLQVERIEELGAQPLRDLIEKNGGWNITGPWDQDNFMEVLKAVAGTYRATPFFTVYISADSKSSNSNVIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMEELGMLLGGRPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQALAPSMDWLEFLSFLLSPLELSDSEPVVVYGMDYLQQVSELINRTEPSILNNYLIWNLVQKTTSSLDRRFESAQEKLLETLYGTKKSCVPRWQTCISNTDDALGFALGSLFVKATFDRQSKEIAEGMISEIRTAFEEALGQLVWMDEKTRQAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEISEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSMTPQTVNAYYLPTKNEIVFPAGILQAPFYARNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLAAFRNHTACMEEQYNQYQVNGERLNGRQTLGENIADNGGLKAAYNAYKAWLRKHGEEQQLPAVGLTNHQLFFVGFAQVWCSVRTPESSHEGLVTDPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNPGQLCEVW
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Predicted Molecular Mass
105 kDa
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Molecular Weight
Approximately 120-130 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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