EED Protein, Human (sf9, His-GST)
EED protein is a core member of the Polycomb group (PcG) and is critical in the PRC2/EED-EZH2 complex, driving "Lys-9" and "Lys-27" methylation on histone H3 to achieve transcriptional repression . It recognizes trimethylated "Lys-26" on histone H1 and inhibits the methyltransferase activity of the PRC2 complex on nucleosomal histone H3 "Lys-27". EED Protein, Human (sf9, His-GST) is the recombinant human-derived EED protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
EED protein is a core member of the Polycomb group (PcG) and is critical in the PRC2/EED-EZH2 complex, driving "Lys-9" and "Lys-27" methylation on histone H3 to achieve transcriptional repression . It recognizes trimethylated "Lys-26" on histone H1 and inhibits the methyltransferase activity of the PRC2 complex on nucleosomal histone H3 "Lys-27". EED Protein, Human (sf9, His-GST) is the recombinant human-derived EED protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
Background
EED, a Polycomb group (PcG) protein, serves as a key component of the PRC2/EED-EZH2 complex, contributing to the methylation of 'Lys-9' and 'Lys-27' residues on histone H3, resulting in the transcriptional repression of target genes. It plays a crucial role in recognizing trimethylated 'Lys-26' of histone H1, inhibiting the methyltransferase activity of the PRC2 complex on nucleosomal histone H3 'Lys-27'. Conversely, recognition of 'Lys-27' trimethylation enables the propagation of this repressive mark. Beyond its role in histone modification, the PRC2/EED-EZH2 complex may act as a platform recruiting DNA methyltransferases, bridging two distinct epigenetic repression systems. Genes subject to repression by this complex include HOXC8, HOXA9, MYT1, and CDKN2A. Comprising EED, EZH2, SUZ12, RBBP4, and RBBP7, and possibly AEBP2, the PRC2/EED-EZH2 complex requires EED, EZH2, and SUZ12 as minimum components for its methyltransferase activity. Furthermore, EED participates in the PRC2/EED-EZH1 complex, which involves EED, EZH1, SUZ12, RBBP4, and AEBP2. The intricate network of interactions, including those with histones, DNMTs, and other proteins, highlights EED's pivotal role in epigenetic regulation.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-His;N-GST
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Accession
O75530 (M1-R441)
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Molecular Construction
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N-term
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His-GST
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EED (M1-R441)
Accession # O75530 -
C-term
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Protein Length
Full Length
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Synonyms
EED; Embryonic Ectoderm Development Protein; Embryonic Ectoderm Development; Alternative Protein EED; HEED; Polycomb Protein Eed; WD Protein Associating With Integrin Cytoplasmic Tails 1; COGIS; Polycomb Protein EED; WAIT1; WAIT-1
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AA Sequence
MSEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTERPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSCENAIVCWKPGKMEDDIDKIKPSESNVTILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAKCTTLTHHKCGAAIRQTSFSRDSSILIAVCDDASIWRWDRLR
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Predicted Molecular Mass
78 kDa
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Molecular Weight
Approximately 75-85 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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