Endoglycan/PODXL2 Protein, Human (HEK293, His)

The endocan/PODXL2 protein serves as a ligand for angioselectin, enabling leukocytes to rapidly roll on the vascular surface through high-affinity divalent cation-dependent interactions with E-, P-, and L-selectin. Through disulfide bonds, the protein forms homodimers and interacts with specific selectins, including SELL, SELE, and SELP. Endoglycan/PODXL2 Protein, Human (HEK293, His) is the recombinant human-derived Endoglycan/PODXL2 protein, expressed by HEK293 , with C-His labeled tag.

Endoglycan/PODXL2 Protein functions as a ligand for vascular selectins, facilitating the rapid rolling of leukocytes over vascular surfaces by engaging in high-affinity divalent cation-dependent interactions with E-, P-, and L-selectins. It forms homodimers through disulfide linkages and interacts with specific selectin proteins, including SELL, SELE, and SELP. These interactions underscore its role in mediating crucial adhesive events that contribute to the intricate processes of leukocyte recruitment and vascular homeostasis.

Human

HEK293

C-8*His

Q9NZ53-1 (G33-T500)

50512  [NCBI]

Extracellular Domain

GSDEPGPEGLTSTSLLDLLLPTGLEPLDSEEPSETMGLGAGLGAPGSGFPSEENEESRILQPPQYFWEEEEELNDSSLDLGPTADYVFPDLTEKAGSIEDTSQAQELPNLPSPLPKMNLVEPPWHMPPREEEEEEEEEEEREKEEVEKQEEEEEEELLPVNGSQEEAKPQVRDFSLTSSSQTPGATKSRHEDSGDQASSGVEVESSMGPSLLLPSVTPTTVTPGDQDSTSQEAEATVLPAAGLGVEFEAPQEASEEATAGAAGLSGQHEEVPALPSFPQTTAPSGAEHPDEDPLGSRTSASSPLAPGDMELTPSSATLGQEDLNQQLLEGQAAEAQSRIPWDSTQVICKDWSNLAGKNYIILNMTENIDCEVFRQHRGPQLLALVEEVLPRHGSGHHGAWHISLSKPSEKEQHLLMTLVGEQGVVPTQDVLSMLGDIRRSLEEIGIQNYSTTSSCQARASQVRSDYGT

70-115 kDa

≥ 95%, as determined by Bis-Tris PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.