Endoglycan/PODXL2 Protein, Human (HEK293, His)
Based on 1 Customer Validation
The endocan/PODXL2 protein serves as a ligand for angioselectin, enabling leukocytes to rapidly roll on the vascular surface through high-affinity divalent cation-dependent interactions with E-, P-, and L-selectin. Through disulfide bonds, the protein forms homodimers and interacts with specific selectins, including SELL, SELE, and SELP. Endoglycan/PODXL2 Protein, Human (HEK293, His) is the recombinant human-derived Endoglycan/PODXL2 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The endocan/PODXL2 protein serves as a ligand for angioselectin, enabling leukocytes to rapidly roll on the vascular surface through high-affinity divalent cation-dependent interactions with E-, P-, and L-selectin. Through disulfide bonds, the protein forms homodimers and interacts with specific selectins, including SELL, SELE, and SELP. Endoglycan/PODXL2 Protein, Human (HEK293, His) is the recombinant human-derived Endoglycan/PODXL2 protein, expressed by HEK293 , with C-His labeled tag.
Background
Endoglycan/PODXL2 Protein functions as a ligand for vascular selectins, facilitating the rapid rolling of leukocytes over vascular surfaces by engaging in high-affinity divalent cation-dependent interactions with E-, P-, and L-selectins. It forms homodimers through disulfide linkages and interacts with specific selectin proteins, including SELL, SELE, and SELP. These interactions underscore its role in mediating crucial adhesive events that contribute to the intricate processes of leukocyte recruitment and vascular homeostasis.
Technical Parameters
-
Species Human
-
Source HEK293
-
Tag C-8*His
-
Accession
Q9NZ53-1 (G33-T500)
-
Molecular Construction
-
N-term
-
Endoglycan (G33-T500)
Accession # Q9NZ53-1 -
8*His
-
C-term
-
-
Protein Length
Extracellular Domain
-
Synonyms
PODXL2; EG; Podocalyxin Like 2; Podocalyxin-Like Protein 2; Endoglycan; Podocalyxin-Like 2; PODLX2
-
AA Sequence
GSDEPGPEGLTSTSLLDLLLPTGLEPLDSEEPSETMGLGAGLGAPGSGFPSEENEESRILQPPQYFWEEEEELNDSSLDLGPTADYVFPDLTEKAGSIEDTSQAQELPNLPSPLPKMNLVEPPWHMPPREEEEEEEEEEEREKEEVEKQEEEEEEELLPVNGSQEEAKPQVRDFSLTSSSQTPGATKSRHEDSGDQASSGVEVESSMGPSLLLPSVTPTTVTPGDQDSTSQEAEATVLPAAGLGVEFEAPQEASEEATAGAAGLSGQHEEVPALPSFPQTTAPSGAEHPDEDPLGSRTSASSPLAPGDMELTPSSATLGQEDLNQQLLEGQAAEAQSRIPWDSTQVICKDWSNLAGKNYIILNMTENIDCEVFRQHRGPQLLALVEEVLPRHGSGHHGAWHISLSKPSEKEQHLLMTLVGEQGVVPTQDVLSMLGDIRRSLEEIGIQNYSTTSSCQARASQVRSDYGT
-
Predicted Molecular Mass
51.5 kDa
-
Molecular Weight
Approximately 85-115 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
-
Glycosylation
Yes
-
Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (238 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
