ENPP-1 Protein, Human (Biotinylated, HEK293, His-Avi)

ENPP-1 protein is a nucleotide pyrophosphatase that inhibits excessive hydroxyapatite crystal growth by preferentially hydrolyzing ATP and other nucleoside triphosphates to generate PPi, thereby critically regulating bone mineralization and soft tissue calcification. ENPP-1 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived ENPP-1 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

ENPP-1 Protein, a nucleotide pyrophosphatase, plays a crucial role in bone mineralization and soft tissue calcification by regulating pyrophosphate levels. By preferentially hydrolyzing ATP and other nucleoside 5' triphosphates, such as GTP, CTP, and UTP, ENPP-1 generates diphosphate (PPi), which inhibits the further growth of nascent hydroxyapatite crystals, thereby preventing excessive bone mineralization and calcification of soft tissues. Additionally, it participates in the hydrolysis of diadenosine polyphosphates and 3',5'-cAMP to AMP, and may regulate nucleotide sugar availability in the endoplasmic reticulum and Golgi, influencing purinergic signaling. ENPP-1 also contributes to the repression of hedgehog signaling, inhibiting ectopic joint calcification and maintaining articular chondrocytes. Furthermore, it is implicated in modulating insulin sensitivity and function, participating in melanogenesis, and can hydrolyze 2',3'-cGAMP, a second messenger involved in triggering type-I interferon production. The exact role of 2',3'-cGAMP hydrolysis in the extracellular space remains unclear.

Immobilized Biotinylated Human ENPP-1, His Tag at 0.5 μg/mL (100 μl/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC₅₀ of 4.5 ng/mL determined by ELISA.

Human

HEK293

C-Avi;C-8*His

P22413 (K98-D925)

5167  [NCBI]

Extracellular Domain

Biotin

KPSCAKEVKSCKGRCFERTFGNCRCDAACVELGNCCLDYQETCIEPEHIWTCNKFRCGEKRLTRSLCACSDDCKDKGDCCINYSSVCQGEKSWVEEPCESINEPQCPAGFETPPTLLFSLDGFRAEYLHTWGGLLPVISKLKKCGTYTKNMRPVYPTKTFPNHYSIVTGLYPESHGIIDNKMYDPKMNASFSLKSKEKFNPEWYKGEPIWVTAKYQGLKSGTFFWPGSDVEINGIFPDIYKMYNGSVPFEERILAVLQWLQLPKDERPHFYTLYLEEPDSSGHSYGPVSSEVIKALQRVDGMVGMLMDGLKELNLHRCLNLILISDHGMEQGSCKKYIYLNKYLGDVKNIKVIYGPAARLRPSDVPDKYYSFNYEGIARNLSCREPNQHFKPYLKHFLPKRLHFAKSDRIEPLTFYLDPQWQLALNPSERKYCGSGFHGSDNVFSNMQALFVGYGPGFKHGIEADTFENIEVYNLMCDLLNLTPAPNNGTHGSLNHLLKNPVYTPKHPKEVHPLVQCPFTRNPRDNLGCSCNPSILPIEDFQTQFNLTVAEEKIIKHETLPYGRPRVLQKENTICLLSQHQFMSGYSQDILMPLWTSYTVDRNDSFSTEDFSNCLYQDFRIPLSPVHKCSFYKNNTKVSYGFLSPPQLNKNSSGIYSEALLTTNIVPMYQSFQVIWRYFHDTLLRKYAEERNGVNVVSGPVFDFDYDGRCDSLENLRQKRRVIRNQEILIPTHFFIVLTSCKDTSQTPLHCENLDTLAFILPHRTDNSESCVHGKHDSSWVEELLMLHRARITDVEHITGLSFYQQRKEPVSDILKLKTHLPTFSQED

110-120 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris,150 mM NaCl, pH 7.4, 8% trehalose.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.