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EphB2 Protein, Human (HEK293, Fc)

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EphB2 Protein is a neurotrophic factor, and a member of the eph receptor tyrosine kinase. EphB2 plays an important role in cancer progression and in immunity. EphB2 can bind to directly to the NMDA receptor and induces its tyrosine phosphorylation. EphB2 is essential for neuronal connectivity and plasticity in the brain, as well as synaptic function. Besides, EphB2 can also interact with cells expressing ephrinB ligands, and results in EphB2 activation and phosphorylation of tyrosines in the ephrinB cytodomain. EphB2 also regulates thrombus formation and clot retraction. EphB2 Protein, Human (HEK293, Fc) is the recombinant human-derived EphB2 protein, expressed by HEK293 , with C-hFc labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for EphB2 Protein, Human (HEK293, Fc)
  • EphB2 Protein, Human (HEK293, Fc) Product Image
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Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

EphB2 Protein is a neurotrophic factor, and a member of the eph receptor tyrosine kinase. EphB2 plays an important role in cancer progression and in immunity. EphB2 can bind to directly to the NMDA receptor and induces its tyrosine phosphorylation. EphB2 is essential for neuronal connectivity and plasticity in the brain, as well as synaptic function. Besides, EphB2 can also interact with cells expressing ephrinB ligands, and results in EphB2 activation and phosphorylation of tyrosines in the ephrinB cytodomain. EphB2 also regulates thrombus formation and clot retraction[1][2][3][4][5]. EphB2 Protein, Human (HEK293, Fc) is the recombinant human-derived EphB2 protein, expressed by HEK293 , with C-hFc labeled tag.

Background

EphB2 protein (Ephrin type-B receptor 2), a neurotrophic factor, is a member of the eph receptor tyrosine kinase. In the gut of rats, EphB2 is expressed preferentially by enteric neurons[1]. EphB2 is also aberrantly expressed in many cancers, and plays an important role in cancer progression. Besides, EphB2 is expressed on some immunocytes, indicating the roles of EphB2 in immunity[2].
EphB2 can bind to directly to the NMDA receptor and induces its tyrosine phosphorylation. It's reported that EphB2 is essential for neuronal connectivity and plasticity in the brain, as well as synaptic function. EphB2 mediates the structural plasticity of neurons associated with the learning of a new behavioral task[1][3]. Besides, EphB2 can also interact with cells expressing ephrinB ligands, and results in EphB2 activation and phosphorylation of tyrosines in the ephrinB cytodomain[4]. EphB2 regulates initial platelet activation in the absence of ligand binding in a contact-independent manner, and EphB2 signaling also regulates thrombus formation and clot retraction[5].
EphB2 is an about 117-kDa transmembrane protein. Human, mouse and rat EphB2 shares more than 98% aa sequence identity.

Verified Bioactivity

This product does not contain protein kinase domain.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-hFc
  • Accession

    Q6NVW1 (V19-S482)

  • Gene ID
    2048  [NCBI]
  • Molecular Construction
    • N-term
    • EphB2 (V19-S482)
      Accession # Q6NVW1
    • hFc
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    EPHB2; Ephrin Type-B Receptor 2 Variant; Prev. EPHT3; Protein-Tyrosine Kinase HEK5; Prev. DRT; Elk-Related Tyrosine Kinase; Prev. ERK; ELK-Related Tyrosine Kinase; Tyrosine-Protein Kinase Receptor EPH-3; Eph Tyrosine Kinase 3; Ephrin Type-B Receptor 2; EP

  • AA Sequence

    VEETLMDSTTATAELGWMVHPPSGWEEVSGYDENMNTIRTYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEMKFSVRDCSSIPSVPGSCKETFNLYYYEADFDSATKTFPNWMENPWVKVDTIAADESFSQVDLGGRVMKINTEVRSFGPVSRSGFYLAFQDYGGCMSLIAVRVFYRKCPRIIQNGAIFQETLSGAESTSLVAARGSCIANAEEVDVPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCRGCPSGTFKANQGDEACTHCPINSRTTSEGATNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDSGGREDLVYNIICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPFSPQFASVNITTNQAAPSAVSIMHQVSRTVDSITLSWSQPDQPNGVILDYELQYYEKMKTQRS

  • Predicted Molecular Mass

    78.5 kDa

  • Molecular Weight

    Approximately 95-120 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
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MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • EphB2 Protein, Human (HEK293, Fc) Product Image
    1/16
  • Experimental Validation Results for EphB2 Protein, Human (HEK293, Fc)
  • EphB2 Protein, Human (HEK293, Fc) Product Image