EphB2 Protein, Human (HEK293, Fc)

EphB2 Protein is a neurotrophic factor, and a member of the eph receptor tyrosine kinase. EphB2 plays an important role in cancer progression and in immunity. EphB2 can bind to directly to the NMDA receptor and induces its tyrosine phosphorylation. EphB2 is essential for neuronal connectivity and plasticity in the brain, as well as synaptic function. Besides, EphB2 can also interact with cells expressing ephrinB ligands, and results in EphB2 activation and phosphorylation of tyrosines in the ephrinB cytodomain. EphB2 also regulates thrombus formation and clot retraction^{[1][2][3][4][5]}. EphB2 Protein, Human (HEK293, Fc) is the recombinant human-derived EphB2 protein, expressed by HEK293, with C-hFc labeled tag. The total length of EphB2 Protein, Human (HEK293, Fc) is 464 a.a., with molecular weight of 95-120 kDa.

EphB2 protein (Ephrin type-B receptor 2), a neurotrophic factor, is a member of the eph receptor tyrosine kinase. In the gut of rats, EphB2 is expressed preferentially by enteric neurons^[1]. EphB2 is also aberrantly expressed in many cancers, and plays an important role in cancer progression. Besides, EphB2 is expressed on some immunocytes, indicating the roles of EphB2 in immunity^[2].
EphB2 can bind to directly to the NMDA receptor and induces its tyrosine phosphorylation. It's reported that EphB2 is essential for neuronal connectivity and plasticity in the brain, as well as synaptic function. EphB2 mediates the structural plasticity of neurons associated with the learning of a new behavioral task^[1][3]. Besides, EphB2 can also interact with cells expressing ephrinB ligands, and results in EphB2 activation and phosphorylation of tyrosines in the ephrinB cytodomain^[4]. EphB2 regulates initial platelet activation in the absence of ligand binding in a contact-independent manner, and EphB2 signaling also regulates thrombus formation and clot retraction^[5].
EphB2 is an about 117-kDa transmembrane protein. Human, mouse and rat EphB2 shares more than 98% aa sequence identity.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-hFc

Q6NVW1 (V19-S482)

2048  [NCBI]

VEETLMDSTTATAELGWMVHPPSGWEEVSGYDENMNTIRTYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEMKFSVRDCSSIPSVPGSCKETFNLYYYEADFDSATKTFPNWMENPWVKVDTIAADESFSQVDLGGRVMKINTEVRSFGPVSRSGFYLAFQDYGGCMSLIAVRVFYRKCPRIIQNGAIFQETLSGAESTSLVAARGSCIANAEEVDVPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCRGCPSGTFKANQGDEACTHCPINSRTTSEGATNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDSGGREDLVYNIICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPFSPQFASVNITTNQAAPSAVSIMHQVSRTVDSITLSWSQPDQPNGVILDYELQYYEKMKTQRS

95-120 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.