EsxA Protein, Mycobacterium tuberculosis (His, Myc)
Based on 1 Customer Validation
EsxA Protein, Mycobacterium tuberculosis (His, Myc) is the recombinant EsxA, expressed by E. coli , with N-10*His, C-Myc labeled tag.
- Species: Others
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
EsxA Protein, Mycobacterium tuberculosis (His, Myc) is the recombinant EsxA, expressed by E. coli , with N-10*His, C-Myc labeled tag.
Background
EsxA is a secreted protein that acts as a potent host T-cell antigen. It plays multiple roles in modulating the host's immune response to infection and is responsible for bacterial escape into the host cytoplasm.
Verified Bioactivity
Measured by its binding ability in a functional ELISA. When Recombinant Mycobacterium tuberculosis EsxA is immobilized at 2.5 µg/mL(100 µL/well) can bind Anti-EsxB antibody. The ED50 for this effect is 468 ng/mL.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
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Bioactivity - ELISA
Bioactivity - ELISA
Technical Parameters
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Species Others
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Source E. coli
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Tag N-10*His;C-Myc
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Accession
P0A565 (T2-A95)
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Gene ID45427879
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Protein Length
Full Length of Mature Protein
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Synonyms
6 kDa early secretory antigenic target; ESAT-6; esxA; esaT6; BQ2027_MB3905
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AA Sequence
TEQQWNFAGIEAAASAIQGNVTSIHSLLDEGKQSLTKLAAAWGGSGSEAYQGVQQKWDATATELNNALQNLARTISEAGQAMASTEGNVTGMFA
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Molecular Weight
Approximately 13 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (235 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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