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FGFR-4 Protein, Human (HEK293, Fc)

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Based on 1 publication(s) in Google Scholar

The leptin R/LEPR protein is a receptor protein that binds to the hormone leptin. It plays a vital role in regulating energy balance and body weight. FGFR-4 Protein, Human (HEK293, Fc) is the recombinant human-derived FGFR-4 protein, expressed by HEK293 , with C-hFc labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for FGFR-4 Protein, Human (HEK293, Fc)
  • Experimental Validation Results for FGFR-4 Protein, Human (HEK293, Fc)
  • FGFR-4 Protein, Human (HEK293, Fc) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

The leptin R/LEPR protein is a receptor protein that binds to the hormone leptin. It plays a vital role in regulating energy balance and body weight. FGFR-4 Protein, Human (HEK293, Fc) is the recombinant human-derived FGFR-4 protein, expressed by HEK293 , with C-hFc labeled tag.

Background

The FGFR-4 Protein serves as a tyrosine kinase and cell surface receptor for fibroblast growth factors, playing a pivotal role in regulating multiple pathways, including cell proliferation, differentiation, migration, lipid metabolism, bile acid biosynthesis, vitamin D metabolism, glucose uptake, and phosphate homeostasis. Structurally, the protein comprises an extracellular region with three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment, and a cytoplasmic tyrosine kinase domain. Through its extracellular portion, which interacts with fibroblast growth factors, FGFR-4 initiates downstream signaling cascades that ultimately influence mitogenesis and differentiation. The gene demonstrates broad expression, with elevated levels observed in the lung (RPKM 16.7), kidney (RPKM 12.5), and 15 other tissues, underscoring its potential significance in diverse physiological contexts across various organs.

Verified Bioactivity

1.This product does not contain protein kinase domain.
2.Measured by its ability to inhibit FGF acidic (aFGF / FGF1) dependent proliferation of Balb/c3T3 mouse embryonic fibroblasts and the ED50 is typically 4-25 ng/mL.
3.Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED50 of this effect is 2.694 ng/mL in the presence of 1ng/mL FGF-1, corresponding to a specific activity is 3.71×105 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED50 of this effect is 2.694 ng/mL in the presence of 1 ng/mL FGF-1, corresponding to a specific activity is 3.71×105 units/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-hFc
  • Accession

    NP_002002.3 (L22-D369)

  • Gene ID
    2264  [NCBI]
  • Molecular Construction
    • N-term
    • FGFR-4 (L22-D369)
      Accession # NP_002002.3
    • hFc
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    FGFR4; Receptor Protein-Tyrosine Kinase; Fibroblast Growth Factor Receptor 4; Hydroxyaryl-Protein Kinase; JTK2; Protein-Tyrosine Kinase; TKF; Soluble FGFR4 Variant 1; CD334; Tyrosylprotein Kinase; Tyrosine Kinase Related To Fibroblast Growth Factor Recept

  • AA Sequence

    LEASEEVELEPCLAPSLEQQEQELTVALGQPVRLCCGRAERGGHWYKEGSRLAPAGRVRGWRGRLEIASFLPEDAGRYLCLARGSMIVLQNLTLITGDSLTSSNDDEDPKSHRDPSNRHSYPQQAPYWTHPQRMEKKLHAVPAGNTVKFRCPAAGNPTPTIRWLKDGQAFHGENRIGGIRLRHQHWSLVMESVVPSDRGTYTCLVENAVGSIRYNYLLDVLERSPHRPILQAGLPANTTAVVGSDVELLCKVYSDAQPHIQWLKHIVINGSSFGADGFPYVQVLKTADINSSEVEVLYLRNVSAEDAGEYTCLAGNSIGLSYQSAWLTVLPEEDPTWTAAAPEARYTD

  • Molecular Weight

    Approximately 80-110 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED50 of this effect is 2.694 ng/mL in the presence of 1 ng/mL FGF-1, corresponding to a specific activity is 3.71×105 units/mg.

  • FGFR-4 Protein, Human (HEK293, Fc) Product Image
    1/16
  • Experimental Validation Results for FGFR-4 Protein, Human (HEK293, Fc)
  • Experimental Validation Results for FGFR-4 Protein, Human (HEK293, Fc)
  • FGFR-4 Protein, Human (HEK293, Fc) Product Image