FGFR-4 Protein, Rhesus Macaque (HEK293, His)

The FGFR-4 protein is a cell surface receptor for tyrosine kinase and fibroblast growth factor. FGFR4, a receptor for FGF-1 and FGF-3 highly expressed in cancer cells, promotes tumor progression in colon cancer by activating Mek/Erk and MMP-7. FGFR-4 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived FGFR-4 protein, expressed by HEK293 , with C-His labeled tag.

FGFR-4 protein, as a cell surface receptor for tyrosine kinase and fibroblast growth factor, plays a key role in regulating multiple pathways, including cell proliferation, differentiation, migration, lipid metabolism, bile acid biosynthesis, vitamin D metabolism, glucose uptake, and phosphate homeostasis. Structurally, the protein consists of an extracellular region with three immunoglobulin-like domains, a hydrophobic membrane span segment, and a cytoplasmic tyrosine kinase domain. Through the extracellular portion of its interaction with fibroblast growth factor, FGFR-4 initiates downstream signaling cascades that ultimately influence mitosis and differentiation. This gene showed widespread expression, with elevated levels observed in the lung (RPKM 16.7), kidney (RPKM 12.5), and 15 other tissues, underscoring its potential significance in different physiological Settings in different organs. FGFR4, a receptor for FGF-1 and FGF-3 highly expressed in cancer cells, promotes tumor progression in colon cancer by activating Mek/Erk and MMP-7^[1][2][3].

1.This product does not contain protein kinase domain.
2.Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED₅₀ for this effect is 3.68 ng/mL in the presence of 2 ng/mL FGF-acidic, corresponding to a specific activity is 2.717×10⁵ units/mg.

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  Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED₅₀ for this effect is 3.68 ng/mL in the presence of 2 ng/mL FGF-acidic, corresponding to a specific activity is 2.717×10⁵ units/mg.

Rhesus Macaque

HEK293

C-6*His

XP_001087243.1 (L22-D369)

698494  [NCBI]

Partial

LEASEEVELEPCLAPSMEQQEQELTVALGQPVRLCCGRAERGGHWYKEGSRLAPAGRVRGWRGRLEIASFLPEDAGRYLCLARASMIVLQNLTLTIDDSLTSSNDDEDPQSHRDSSNGHIYPQQAPYWTHPQRMEKKLHAVPAGNTVKFRCPAAGNPTPTIRWLKDGQAFHGENRIGGIRLRHQHWSLVMESVVPSDRGTYTCLVENAVGIIRYNYLLDVLERSPHRPILQAGLPANTTAVVGSDVELLCKVYSDAQPHIQWLKHIVINGSSFGADGFPYVQVLKTADINSSEVEVLYLRNVSAEDAGEYTCLAGNSIGLSYQSAWLTVLPEEDLTWTAATPEARYTD

Approximately 60-80 kDa

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Bai YP, et al. FGF-1/-3/FGFR4 signaling in cancer-associated fibroblasts promotes tumor progression in colon cancer through Erk and MMP-7. Cancer Sci. 2015 Oct;106(10):1278-87.  [Content Brief]

  [2]. Stark KL, et al. FGFR-4, a new member of the fibroblast growth factor receptor family, expressed in the definitive endoderm and skeletal muscle lineages of the mouse. Development. 1991 Oct;113(2):641-51.  [Content Brief]

  [3]. Yamada SM, et al. Fibroblast growth factor receptor (FGFR) 4 correlated with the malignancy of human astrocytomas. Neurol Res. 2002 Apr;24(3):244-8.  [Content Brief]