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  4. FLRT2 Protein, Mouse (HEK293, His)

FLRT2 Protein, Mouse (HEK293, His)

Cat. No.: HY-P76934
SDS COA Handling Instructions

The FLRT2 protein plays a key role in cell-cell adhesion, migration, and axonal guidance. Its interaction with ADGRL3 promotes cell-cell adhesion and promotes cortical neuronal migration through interaction with UNC5D. FLRT2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived FLRT2 protein, expressed by HEK293 , with C-His labeled tag. The total length of FLRT2 Protein, Mouse (HEK293, His) is 504 a.a., with molecular weight of 60-80 kDa.

For research use only. We do not sell to patients.

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2 μg $30 In-stock
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  • Biological Activity

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Description

The FLRT2 protein plays a key role in cell-cell adhesion, migration, and axonal guidance. Its interaction with ADGRL3 promotes cell-cell adhesion and promotes cortical neuronal migration through interaction with UNC5D. FLRT2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived FLRT2 protein, expressed by HEK293 , with C-His labeled tag. The total length of FLRT2 Protein, Mouse (HEK293, His) is 504 a.a., with molecular weight of 60-80 kDa.

Background

FLRT2 protein exerts a crucial role in diverse cellular processes, including cell-cell adhesion, cell migration, and axon guidance. Its interaction with ADGRL3 and potentially other latrophilins expressed on adjacent cells facilitates cell-cell adhesion, while also playing a role in the migration of cortical neurons during brain development through interaction with UNC5D. In axon guidance, FLRT2 mediates growth cone collapse and exhibits a repulsive effect on neuron guidance, interacting with UNC5D and potentially other UNC-5 family members. Additionally, FLRT2 is implicated in fibroblast growth factor-mediated signaling cascades. Vital for normal cardiac basement membrane organization during embryogenesis, FLRT2 is also required for proper embryonic epicardium and heart morphogenesis. Through self-association via leucine-rich repeats, FLRT2 forms homooligomers and interacts with various partners, including FGFR1, FGFR2, ADGRL1/LPHN1, ADGRL3, UNC5D, UNC5B, and FN1.

Biological Activity

Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells. The ED50 for this effect is 0.8205 µg/mL, corresponding to a specific activity is 1.219×103 units/mg.

  • Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells. The ED50 for this effect is 0.8205 µg/mL, corresponding to a specific activity is 1.219×103 units/mg.
Species

Mouse

Source

HEK293

Tag

C-10*His

Accession

Q8BLU0 (C36-S539)

Gene ID
Molecular Construction
N-term
FLRT2 (C36-S539)
Accession # Q8BLU0
His
C-term
Synonyms
Leucine-rich repeat transmembrane protein FLRT2; Flrt2; Kiaa0405
AA Sequence

CPSVCRCDRNFVYCNERSLTSVPLGIPEGVTVLYLHNNQINNAGFPAELHNVQSVHTVYLYGNQLDEFPMNLPKNVRVLHLQENNIQTISRAALAQLLKLEELHLDDNSISTVGVEDGAFREAISLKLLFLSKNHLSSVPVGLPVDLQELRVDENRIAVISDMAFQNLTSLERLIVDGNLLTNKGIAEGTFSHLTKLKEFSIVRNSLSHPPPDLPGTHLIRLYLQDNQINHIPLTAFANLRKLERLDISNNQLRMLTQGVFDHLSNLKQLTARNNPWFCDCSIKWVTEWLKYIPSSLNVRGFMCQGPEQVRGMAVRELNMNLLSCPTTTPGLPVFTPAPSTVSPTTQSPTLSVPSPSRGSVPPAPTPSKLPTIPDWDGRERVTPPISERIQLSIHFVNDTSIQVSWLSLFTVMAYKLTWVKMGHSLVGGIVQERIVSGEKQHLSLVNLEPRSTYRICLVPLDAFNYRTVEDTICSEATTHASYLNNGSNTASSHEQTTSHSMGS

Molecular Weight

Approximately 60-80 kDa due to the glycosylation.

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

FLRT2 Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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FLRT2 Protein, Mouse (HEK293, His)
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HY-P76934
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