FLRT2 Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
The FLRT2 protein plays a key role in cell-cell adhesion, migration, and axonal guidance. Its interaction with ADGRL3 promotes cell-cell adhesion and promotes cortical neuronal migration through interaction with UNC5D. FLRT2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived FLRT2 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The FLRT2 protein plays a key role in cell-cell adhesion, migration, and axonal guidance. Its interaction with ADGRL3 promotes cell-cell adhesion and promotes cortical neuronal migration through interaction with UNC5D. FLRT2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived FLRT2 protein, expressed by HEK293 , with C-His labeled tag.
FLRT2 protein exerts a crucial role in diverse cellular processes, including cell-cell adhesion, cell migration, and axon guidance. Its interaction with ADGRL3 and potentially other latrophilins expressed on adjacent cells facilitates cell-cell adhesion, while also playing a role in the migration of cortical neurons during brain development through interaction with UNC5D. In axon guidance, FLRT2 mediates growth cone collapse and exhibits a repulsive effect on neuron guidance, interacting with UNC5D and potentially other UNC-5 family members. Additionally, FLRT2 is implicated in fibroblast growth factor-mediated signaling cascades. Vital for normal cardiac basement membrane organization during embryogenesis, FLRT2 is also required for proper embryonic epicardium and heart morphogenesis. Through self-association via leucine-rich repeats, FLRT2 forms homooligomers and interacts with various partners, including FGFR1, FGFR2, ADGRL1/LPHN1, ADGRL3, UNC5D, UNC5B, and FN1.
Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells. The ED50 for this effect is 0.8205 µg/mL, corresponding to a specific activity is 1.219×103 units/mg.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-10*His
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Accession
Q8BLU0 (C36-S539)
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Molecular Construction
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N-term
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FLRT2 (C36-S539)
Accession # Q8BLU0 -
His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
FLRT2; Leucine-Rich Repeat Transmembrane Protein FLRT2; Fibronectin Leucine Rich Transmembrane Protein 2; KIAA0405; Fibronectin-Like Domain-Containing Leucine-Rich Transmembrane Protein 2
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AA Sequence
CPSVCRCDRNFVYCNERSLTSVPLGIPEGVTVLYLHNNQINNAGFPAELHNVQSVHTVYLYGNQLDEFPMNLPKNVRVLHLQENNIQTISRAALAQLLKLEELHLDDNSISTVGVEDGAFREAISLKLLFLSKNHLSSVPVGLPVDLQELRVDENRIAVISDMAFQNLTSLERLIVDGNLLTNKGIAEGTFSHLTKLKEFSIVRNSLSHPPPDLPGTHLIRLYLQDNQINHIPLTAFANLRKLERLDISNNQLRMLTQGVFDHLSNLKQLTARNNPWFCDCSIKWVTEWLKYIPSSLNVRGFMCQGPEQVRGMAVRELNMNLLSCPTTTPGLPVFTPAPSTVSPTTQSPTLSVPSPSRGSVPPAPTPSKLPTIPDWDGRERVTPPISERIQLSIHFVNDTSIQVSWLSLFTVMAYKLTWVKMGHSLVGGIVQERIVSGEKQHLSLVNLEPRSTYRICLVPLDAFNYRTVEDTICSEATTHASYLNNGSNTASSHEQTTSHSMGS
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Molecular Weight
Approximately 60-80 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (239 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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