FLRT2 Protein, Mouse (HEK293, His)

The FLRT2 protein plays a key role in cell-cell adhesion, migration, and axonal guidance. Its interaction with ADGRL3 promotes cell-cell adhesion and promotes cortical neuronal migration through interaction with UNC5D. FLRT2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived FLRT2 protein, expressed by HEK293 , with C-His labeled tag. The total length of FLRT2 Protein, Mouse (HEK293, His) is 504 a.a., with molecular weight of 60-80 kDa.

FLRT2 protein exerts a crucial role in diverse cellular processes, including cell-cell adhesion, cell migration, and axon guidance. Its interaction with ADGRL3 and potentially other latrophilins expressed on adjacent cells facilitates cell-cell adhesion, while also playing a role in the migration of cortical neurons during brain development through interaction with UNC5D. In axon guidance, FLRT2 mediates growth cone collapse and exhibits a repulsive effect on neuron guidance, interacting with UNC5D and potentially other UNC-5 family members. Additionally, FLRT2 is implicated in fibroblast growth factor-mediated signaling cascades. Vital for normal cardiac basement membrane organization during embryogenesis, FLRT2 is also required for proper embryonic epicardium and heart morphogenesis. Through self-association via leucine-rich repeats, FLRT2 forms homooligomers and interacts with various partners, including FGFR1, FGFR2, ADGRL1/LPHN1, ADGRL3, UNC5D, UNC5B, and FN1.

Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells. The ED₅₀ for this effect is 0.8205 µg/mL, corresponding to a specific activity is 1.219×10³ units/mg.

Mouse

HEK293

C-10*His

Q8BLU0 (C36-S539)

399558  [NCBI]

CPSVCRCDRNFVYCNERSLTSVPLGIPEGVTVLYLHNNQINNAGFPAELHNVQSVHTVYLYGNQLDEFPMNLPKNVRVLHLQENNIQTISRAALAQLLKLEELHLDDNSISTVGVEDGAFREAISLKLLFLSKNHLSSVPVGLPVDLQELRVDENRIAVISDMAFQNLTSLERLIVDGNLLTNKGIAEGTFSHLTKLKEFSIVRNSLSHPPPDLPGTHLIRLYLQDNQINHIPLTAFANLRKLERLDISNNQLRMLTQGVFDHLSNLKQLTARNNPWFCDCSIKWVTEWLKYIPSSLNVRGFMCQGPEQVRGMAVRELNMNLLSCPTTTPGLPVFTPAPSTVSPTTQSPTLSVPSPSRGSVPPAPTPSKLPTIPDWDGRERVTPPISERIQLSIHFVNDTSIQVSWLSLFTVMAYKLTWVKMGHSLVGGIVQERIVSGEKQHLSLVNLEPRSTYRICLVPLDAFNYRTVEDTICSEATTHASYLNNGSNTASSHEQTTSHSMGS

Approximately 60-80 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.