Fumarate hydratase/FH Protein, Human
The fumarate hydratase/FH protein plays a key role in cellular processes as it catalyzes the hydration of fumarate to L-malate in the tricarboxylic acid (TCA) cycle, which is essential for energy production through the production of NADH . Fumarate hydratase/FH Protein, Human is the recombinant human-derived Fumarate hydratase/FH protein, expressed by E. coli , with tag free.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The fumarate hydratase/FH protein plays a key role in cellular processes as it catalyzes the hydration of fumarate to L-malate in the tricarboxylic acid (TCA) cycle, which is essential for energy production through the production of NADH . Fumarate hydratase/FH Protein, Human is the recombinant human-derived Fumarate hydratase/FH protein, expressed by E. coli , with tag free.
Background
Fumarate Hydratase (FH) is an enzyme that catalyzes the reversible conversion of fumarate to L-malate. This stereospecific interconversion plays a pivotal role in the tricarboxylic acid (TCA) cycle, a central metabolic pathway involved in energy production. Specifically, FH facilitates the hydration of fumarate to L-malate, contributing to a key transition step in the TCA cycle. Experiments in various species suggest that specific isoforms of FH may act in defined pathways and exhibit preferences for one direction of the reaction over the other. By participating in the TCA cycle, FH ensures the efficient generation of energy in the form of NADH, highlighting its essential role in cellular metabolism.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source E. coli
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Tag Tag Free
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Accession
P07954-1 (A45-K510)
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Molecular Construction
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N-term
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FH (A45-K510)
Accession # P07954-1 -
C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
FH; Fumarate Hydratase Isoform 4; Fumarate Hydratase; Fumarate Hydratase Isoform 7; Fumarate Hydratase, Mitochondrial; Fumarate Hydratase Isoform 5; Fumarase; HLRCC; HsFH; MCUL1; Epididymis Secretory Sperm Binding Protein; FMRD; Fumarate Hydratase Isoform
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AA Sequence
ASQNSFRIEYDTFGELKVPNDKYYGAQTVRSTMNFKIGGVTERMPTPVIKAFGILKRAAAEVNQDYGLDPKIANAIMKAADEVAEGKLNDHFPLVVWQTGSGTQTNMNVNEVISNRAIEMLGGELGSKIPVHPNDHVNKSQSSNDTFPTAMHIAAAIEVHEVLLPGLQKLHDALDAKSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK
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Predicted Molecular Mass
50.2 kDa
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Molecular Weight
Approximately 45 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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