Furin/PCSK3 Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
Furin/PCSK3 protein is an endoprotease with RX(K/R)R cleavage activity and is widely expressed in the constitutive secretory pathway.It plays a crucial role in processing various substrates, including TGFB1, and activating TGF-β-1.Furin/PCSK3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Furin/PCSK3 protein, expressed by HEK293 , with C-10*His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Furin/PCSK3 protein is an endoprotease with RX(K/R)R cleavage activity and is widely expressed in the constitutive secretory pathway.It plays a crucial role in processing various substrates, including TGFB1, and activating TGF-β-1.Furin/PCSK3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Furin/PCSK3 protein, expressed by HEK293 , with C-10*His labeled tag.
Background
Furin/PCSK3 protein is a widely expressed endoprotease present in constitutive secretory pathways that exhibits cleavage activity at the RX(K/R)R consensus motif. It plays a crucial role in the processing of various substrates, including TGFB1, contributing to the activation of TGF-beta-1. Additionally, Furin/PCSK3 protein is responsible for converting the non-functional prohormone Brain natriuretic factor into its active hormone BNP(1-45) through proteolytic cleavage. Moreover, it regulates the acidification of dense-core secretory granules in islets of Langerhans cells by mediating the processing of the accessory subunit ATP6AP1/Ac45 of the V-ATPase.
Verified Bioactivity
1.Measured by its ability to cleave the fluorogenic peptide substrate pERTKR-AMC. The specific activity is 307.98 pmol/min/µg, as measured under the described conditions.
2.Measured by its ability to cleave 50µM fluorogenic peptide substrate Pyr-Arg-Thr-Lys-Arg-AMC that incubate at room temperature for 5minutes.The specific activity is 1653.016 pmol/min/µg.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-10*His
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Accession
P23188 (Q25-E714)
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Molecular Construction
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N-term
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Furin (Q25-E714)
Accession # P23188 -
10*His
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C-term
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Protein Length
Lumenal Domain
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Synonyms
FURIN; Subtilisin-Like Proprotein Convertase 1; Prev. PCSK3; Paired Basic Amino Acid Cleaving Enzyme (Furin, Membrane Associated Receptor Protein); Prev. PACE; Furin (Paired Basic Amino Acid Cleaving Enzyme); Prev. FUR; Proprotein Convertase Subtilisin/Ke
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AA Sequence
QGQKIFTNTWAVHIPGGPAVADRVAQKHGFHNLGQIFGDYYHFWHRAVTKRSLSPHRPRHSRLQREPQVKWLEQQVAKRRAKRDVYQEPTDPKFPQQWYLSGVTQRDLNVKEAWAQGFTGHGIVVSILDDGIEKNHPDLAGNYDPGASFDVNDQDPDPQPRYTQMNDNRHGTRCAGEVAAVANNGVCGVGVAYNARIGGVRMLDGEVTDAVEARSLGLNPNHIHIYSASWGPEDDGKTVDGPARLAEEAFFRGVSQGRGGLGSIFVWASGNGGREHDSCNCDGYTNSIYTLSISSATQFGNVPWYSEACSSTLATTYSSGNQNEKQIVTTDLRQKCTESHTGTSASAPLAAGIIALTLEANKNLTWRDMQHLVVQTSKPAHLNADDWATNGVGRKVSHSYGYGLLDAGAMVALAQNWTTVAPQRKCIVEILVEPKDIGKRLEVRKAVTACLGEPNHITRLEHVQARLTLSYNRRGDLAIHLISPMGTRSTLLAARPHDYSADGFNDWAFMTTHSWDEDPAGEWVLEIENTSEANNYGTLTKFTLVLYGTAPEGLSTPPESSGCKTLTSSQACVVCEEGYSLHQKSCVQHCPPGFIPQVLDTHYSTENDVEIIRASVCTPCHASCATCQGPAPTDCLSCPSHASLDPVEQTCSRQSQSSRESRPQQQPPALRPEVEMEPRLQAGLASHLPE
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Molecular Weight
Approximately 60-80 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCL, 150 mM NaCl, 1 mM CaCl2, 0.5% (w/v) Brij-35, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (240 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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