GAD2/GAD65 Protein, Human (sf9, GST)

GAD2/GAD65 proteins are key enzymes in neurotransmitter metabolism, specifically catalyzing the production of gamma-aminobutyric acid (GABA). GABA is an important inhibitory neurotransmitter in the central nervous system, playing a key role in regulating neuronal excitability and maintaining the balance between excitation and inhibition in the brain. GAD2/GAD65 Protein, Human (sf9, GST) is the recombinant human-derived GAD2/GAD65 protein, expressed by Sf9 insect cells , with N-GST labeled tag.

The GAD2, also known as GAD65 (Glutamate Decarboxylase 2), protein is an enzyme that plays a crucial role in neurotransmitter regulation by catalyzing the conversion of glutamate to gamma-aminobutyric acid (GABA). GAD2/GAD65 is a major isoform of glutamate decarboxylase, and its enzymatic activity is vital for maintaining the balance between excitatory and inhibitory neurotransmission in the central nervous system. GABA serves as the primary inhibitory neurotransmitter, and its synthesis by GAD2/GAD65 contributes to the modulation of neuronal excitability. Dysregulation of GAD2 activity has been implicated in various neurological disorders, underscoring the significance of this enzyme in neural function and highlighting its potential as a therapeutic target.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-GST

Q05329/NP_000809.1 (M1-L585)

2572  [NCBI]

Full Length

MASPGSGFWSFGSEDGSGDSENPGTARAWCQVAQKFTGGIGNKLCALLYGDAEKPAESGGSQPPRAAARKAACACDQKPCSCSKVDVNYAFLHATDLLPACDGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWELADQPQNLEEILMHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWLTSTANTNMFTYEIAPVFVLLEYVTLKKMREIIGWPGGSGDGIFSPGGAISNMYAMMIARFKMFPEVKEKGMAALPRLIAFTSEHSHFSLKKGAAALGIGTDSVILIKCDERGKMIPSDLERRILEAKQKGFVPFLVSATAGTTVYGAFDPLLAVADICKKYKIWMHVDAAWGGGLLMSRKHKWKLSGVERANSVTWNPHKMMGVPLQCSALLVREEGLMQNCNQMHASYLFQQDKHYDLSYDTGDKALQCGRHVDVFKLWLMWRAKGTTGFEAHVDKCLELAEYLYNIIKNREGYEMVFDGKPQHTNVCFWYIPPSLRTLEDNEERMSRLSKVAPVIKARMMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL

Approximately 92.6 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 200 mM Tris, 150 mM NaCl, 10% Glycerol, 1 mM GSH, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.