GAD2/GAD65 Protein, Human (sf9, GST)
Based on 1 Customer Validation
GAD2/GAD65 proteins are key enzymes in neurotransmitter metabolism, specifically catalyzing the production of gamma-aminobutyric acid (GABA). GABA is an important inhibitory neurotransmitter in the central nervous system, playing a key role in regulating neuronal excitability and maintaining the balance between excitation and inhibition in the brain. GAD2/GAD65 Protein, Human (sf9, GST) is the recombinant human-derived GAD2/GAD65 protein, expressed by Sf9 insect cells , with N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
GAD2/GAD65 proteins are key enzymes in neurotransmitter metabolism, specifically catalyzing the production of gamma-aminobutyric acid (GABA). GABA is an important inhibitory neurotransmitter in the central nervous system, playing a key role in regulating neuronal excitability and maintaining the balance between excitation and inhibition in the brain. GAD2/GAD65 Protein, Human (sf9, GST) is the recombinant human-derived GAD2/GAD65 protein, expressed by Sf9 insect cells , with N-GST labeled tag.
Background
The GAD2, also known as GAD65 (Glutamate Decarboxylase 2), protein is an enzyme that plays a crucial role in neurotransmitter regulation by catalyzing the conversion of glutamate to gamma-aminobutyric acid (GABA). GAD2/GAD65 is a major isoform of glutamate decarboxylase, and its enzymatic activity is vital for maintaining the balance between excitatory and inhibitory neurotransmission in the central nervous system. GABA serves as the primary inhibitory neurotransmitter, and its synthesis by GAD2/GAD65 contributes to the modulation of neuronal excitability. Dysregulation of GAD2 activity has been implicated in various neurological disorders, underscoring the significance of this enzyme in neural function and highlighting its potential as a therapeutic target.
Verified Bioactivity
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-GST
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Accession
Q05329/NP_000809.1 (M1-L585)
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Molecular Construction
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N-term
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GST
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GAD2 (M1-L585)
Accession # Q05329/NP_000809.1 -
C-term
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Protein Length
Full Length
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Synonyms
GAD2; Glutamate Decarboxylase 65 KDa Isoform; Glutamate Decarboxylase 2; GAD-65; GAD65; Glutamate Decarboxylase 2 (Pancreatic Islets And Brain, 65kD); 65 KDa Glutamic Acid Decarboxylase; Glutamate Decarboxylase-2 (Pancreas); Glutamate Decarboxylase 2 (Pancreatic Islets And Brain, 65kDa); Glutamate Decarboxylase
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AA Sequence
MASPGSGFWSFGSEDGSGDSENPGTARAWCQVAQKFTGGIGNKLCALLYGDAEKPAESGGSQPPRAAARKAACACDQKPCSCSKVDVNYAFLHATDLLPACDGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWELADQPQNLEEILMHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWLTSTANTNMFTYEIAPVFVLLEYVTLKKMREIIGWPGGSGDGIFSPGGAISNMYAMMIARFKMFPEVKEKGMAALPRLIAFTSEHSHFSLKKGAAALGIGTDSVILIKCDERGKMIPSDLERRILEAKQKGFVPFLVSATAGTTVYGAFDPLLAVADICKKYKIWMHVDAAWGGGLLMSRKHKWKLSGVERANSVTWNPHKMMGVPLQCSALLVREEGLMQNCNQMHASYLFQQDKHYDLSYDTGDKALQCGRHVDVFKLWLMWRAKGTTGFEAHVDKCLELAEYLYNIIKNREGYEMVFDGKPQHTNVCFWYIPPSLRTLEDNEERMSRLSKVAPVIKARMMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL
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Predicted Molecular Mass
92.6 kDa
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Molecular Weight
Approximately 80-90 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 200 mM Tris, 150 mM NaCl, 10% Glycerol, 1 mM GSH, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (239 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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