GARP&Latent TGF beta Complex Protein, Mouse (Biotinylated, HEK293, His-Avi)

GARP/latent TGF-b1 complexes are potent inducers of Th17 differentiation in the presence of exogenous IL-6 and inducers of Treg in the presence of IL-2. GARP&Latent TGF beta Complex Protein, Mouse (Biotinylated, HEK293, His-Avi) is a biotinylated recombinant protein (GARP (I18-N628)&TGF-b1 (L30-S390)) produced by HEK293 cells with His-Avi tag^[1][2].

GARP/latent TGF-b1 complexes are potent inducers of Th17 differentiation in the presence of exogenous IL-6 and inducers of Treg in the presence of IL-2^[1][2].
GARP/LTGFβ1 complex is expressed at very low levels on freshly explanted Treg, but markedly upregulated following Treg activation in vitro. GARP/Latent TGF-β1 complex on Treg cells modulates the induction of peripherally derived Treg cells during oral tolerance^[3]

Immobilized Mouse ITGAV&ITGB6, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse GARP&Latent TGF beta 1 Complex, His Tag with the EC₅₀ of 94.4 ng/ml determined by ELISA.

Mouse

HEK293

N-Avi;N-8*His

G3XA59 (I18-N628)&P04202 (L30-S390)

434215  [NCBI]&21803  [NCBI]

Biotin

The single lysine residue in Avitag is biotinylated through an enzymatic reaction.

72-76 kDa (GARP), 13 kDa and 45-47 kDa (Latent TGF beta 1)

≥ 95%, as determined by Bis-Tris PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Soyoung A Oh,TGF-β: guardian of T cell function. J Immunol. 2013 Oct 15;191(8):3973-9.  [Content Brief]

  [2]. Angela X Zhou, et al. GARP-TGF-β complexes negatively regulate regulatory T cell development and maintenance of peripheral CD4+ T cells in vivo. J Immunol. 2013 May 15;190(10):5057-64.  [Content Brief]

  [3]. Justin P Edwards, et al. The GARP/Latent TGF-β1 complex on Treg cells modulates the induction of peripherally derived Treg cells during oral tolerance. Eur J Immunol. 2016 Jun;46(6):1480-9.  [Content Brief]