GASP-1/WFIKKN2 Protein, Human (HEK293, His)

GASP-1/WFIKKN2, a multifaceted protease inhibitor with distinct domains, potentially inhibits serine proteases and metalloproteases. Its intriguing role involves inhibiting mature myostatin activity, leaving activin unaffected. GASP-1/WFIKKN2's engagement with both mature and propeptide forms of myostatin/MSTN adds complexity to the regulatory landscape of protease activities in biological systems. GASP-1/WFIKKN2 Protein, Human (HEK293, His) is the recombinant human-derived GASP-1/WFIKKN2 protein, expressed by HEK293 , with C-His labeled tag.

GASP-1/WFIKKN2 is a multifaceted protease inhibitor that possesses multiple distinct domains and exhibits a range of potential inhibitory activities against serine proteases and metalloproteases. This protein exerts an inhibitory effect on the biological activity of mature myostatin, while activin is unaffected. GASP-1/WFIKKN2 binds to both the mature and propeptide forms of myostatin/MSTN, thereby contributing to the complex regulation of protease activity in biological systems.

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (HY-P2185A) that incubate at room temperature. The IC₅₀ is 0.969 nM.

Human

HEK293

C-His

Q8TEU8 (L35-H576)

124857  [NCBI]

Full Length of Mature Protein

LPPIRYSHAGICPNDMNPNLWVDAQSTCRRECETDQECETYEKCCPNVCGTKSCVAARYMDVKGKKGPVGMPKEATCDHFMCLQQGSECDIWDGQPVCKCKDRCEKEPSFTCASDGLTYYNRCYMDAEACSKGITLAVVTCRYHFTWPNTSPPPPETTMHPTTASPETPELDMAAPALLNNPVHQSVTMGETVSFLCDVVGRPRPEITWEKQLEDRENVVMRPNHVRGNVVVTNIAQLVIYNAQLQDAGIYTCTARNVAGVLRADFPLSVVRGHQAAATSESSPNGTAFPAAECLKPPDSEDCGEEQTRWHFDAQANNCLTFTFGHCHRNLNHFETYEACMLACMSGPLAACSLPALQGPCKAYAPRWAYNSQTGQCQSFVYGGCEGNGNNFESREACEESCPFPRGNQRCRACKPRQKLVTSFCRSDFVILGRVSELTEEPDSGRALVTVDEVLKDEKMGLKFLGQEPLEVTLLHVDWACPCPNVTVSEMPLIIMGEVDGGMAMLRPDSFVGASSARRVRKLREVMHKKTCDVLKEFLGLH

Approximately 70-75 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.