GRK6 Protein, Human (Active, sf9, His-GST)
Based on 1 Customer Validation
GRK6 selectively phosphorylates activated G protein-coupled receptors, triggering beta-arrestin-mediated desensitization, internalization, and signaling events. It is implicated in desensitizing D2-like dopamine receptors in the striatum and chemokine receptor CXCR4, crucial for CXCL12-induced cell chemotaxis. Additionally, GRK6 phosphorylates rhodopsin (RHO) and the non-GPCR LRP6 during Wnt signaling, as observed in vitro. GRK6 Protein, Human (sf9, His-GST) is the recombinant human-derived GRK6 protein, expressed by Sf9 insect cells , with N-GST, N-His labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
GRK6 selectively phosphorylates activated G protein-coupled receptors, triggering beta-arrestin-mediated desensitization, internalization, and signaling events. It is implicated in desensitizing D2-like dopamine receptors in the striatum and chemokine receptor CXCR4, crucial for CXCL12-induced cell chemotaxis. Additionally, GRK6 phosphorylates rhodopsin (RHO) and the non-GPCR LRP6 during Wnt signaling, as observed in vitro. GRK6 Protein, Human (sf9, His-GST) is the recombinant human-derived GRK6 protein, expressed by Sf9 insect cells , with N-GST, N-His labeled tag.
Background
GRK6 is a protein that selectively phosphorylates activated forms of G protein-coupled receptors, initiating downstream processes such as beta-arrestin-mediated receptor desensitization, internalization, and signaling events crucial for their functional downregulation. It is implicated in the desensitization of D2-like dopamine receptors in the striatum and the chemokine receptor CXCR4, playing a pivotal role in CXCL12-induced cell chemotaxis. Additionally, GRK6 has been shown to phosphorylate rhodopsin (RHO) in vitro and a non G-protein-coupled receptor, LRP6, during Wnt signaling, suggesting its involvement in diverse cellular pathways.
Verified Bioactivity
The specific activity was determined to be >7 nmol/min/mg using casein as substrate.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-GST;N-His
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Accession
P43250-2 (M1-R589)
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Molecular Construction
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N-term
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His-GST
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GRK6 (M1-R589)
Accession # P43250-2 -
C-term
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Protein Length
Full Length of Isoform-2
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Synonyms
GRK6; G Protein-Coupled Receptor Kinase GRK6; Prev. GPRK6; G Protein-Coupled Receptor Kinase 6; G Protein-Coupled Receptor Kinase
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AA Sequence
MELENIVANTVLLKAREGGGGNRKGKSKKWRQMLQFPHISQCEELRLSLERDYHSLCERQPIGRLLFREFCATRPELSRCVAFLDGVAEYEVTPDDKRKACGRQLTQNFLSHTGPDLIPEVPRQLVTNCTQRLEQGPCKDLFQELTRLTHEYLSVAPFADYLDSIYFNRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRIKKRKGEAMALNEKQILEKVNSRFVVSLAYAYETKDALCLVLTLMNGGDLKFHIYHMGQAGFPEARAVFYAAEICCGLEDLHRERIVYRDLKPENILLDDHGHIRISDLGLAVHVPEGQTIKGRVGTVGYMAPEVVKNERYTFSPDWWALGCLLYEMIAGQSPFQQRKKKIKREEVERLVKEVPEEYSERFSPQARSLCSQLLCKDPAERLGCRGGSAREVKEHPLFKKLNFKRLGAGMLEPPFKPDPQAIYCKDVLDIEQFSTVKGVELEPTDQDFYQKFATGSVPIPWQNEMVETECFQELNVFGLDGSVPPDLDWKGQPPAPPKKGLLQRLFSRQRIAVETAATARKSSPPASSPQPEAPTSSWR
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Predicted Molecular Mass
95.1 kDa
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Molecular Weight
Approximately 85 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.5, 10% glycerol, 0.5 mM TCEP, 2 mM GSH.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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