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  3. Cytokines and Growth Factors
  4. HDGF Protein, Mouse (P. pastoris, N-His, C-Myc)

HDGF Protein, Mouse (P. pastoris, N-His, C-Myc)

Cat. No.: HY-P700488
Handling Instructions

HDGF Protein, a transcriptional repressor, promotes fibroblast mitogenic activity. Existing as a monomer or domain-swapped homodimer, it interacts with nuclear proteins NCL and YBX1/YB1. HDGF Protein, Mouse (P. pastoris, N-His, C-Myc) is the recombinant mouse-derived HDGF protein, expressed by P. pastoris, with C-Myc, N-10*His labeled tag. The total length of HDGF Protein, Mouse (P. pastoris, N-His, C-Myc) is 237 a.a., with molecular weight of 30.3 kDa.

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Description

HDGF Protein, a transcriptional repressor, promotes fibroblast mitogenic activity. Existing as a monomer or domain-swapped homodimer, it interacts with nuclear proteins NCL and YBX1/YB1. HDGF Protein, Mouse (P. pastoris, N-His, C-Myc) is the recombinant mouse-derived HDGF protein, expressed by P. pastoris, with C-Myc, N-10*His labeled tag. The total length of HDGF Protein, Mouse (P. pastoris, N-His, C-Myc) is 237 a.a., with molecular weight of 30.3 kDa.
Background

HDGF Protein functions as a transcriptional repressor and exhibits mitogenic activity for fibroblasts. This heparin-binding protein can exist as a monomer and as a domain-swapped homodimer. Additionally, it interacts with nuclear proteins NCL and YBX1/YB1.
Species

Mouse
Source

P. pastoris
Tag

C-Myc;N-10*His
Accession

P51859 (M1-L237)
Gene ID

15191  [NCBI]
Molecular Construction
N-term
10*His
HDGF (M1-L237)
Accession # P51859
Myc
C-term
Synonyms
rHuHepatoma-derived growth factor/HDGF, His; Hepatoma-Derived Growth Factor; HDGF; High Mobility Group Protein 1-Kike 2; HMG-1L2; HDGF; HMG1L2
AA Sequence

MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCAAEPEVEPEAHEGDGDKKGSAEGSSDEEGKLVIDEPAKEKNEKGTLKRRAGDVLEDSPKRPKESGDHEEEDKEIAALEGERPLPVEVEKNSTPSEPDSGQGPPAEEEEGEEEAAKEEAEAQGVRDHESL

Molecular Weight

30.3 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

HDGF Protein, Mouse (P. pastoris, N-His, C-Myc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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