HGFA/HGF Activator Protein, Human (HEK293, His)

HGFA/HGF-activating proteins play a crucial role by catalyzing the activation of hepatocyte growth factor (HGF) and converting it into biologically active heterodimers. The process involves the formation of heterodimers with short and long chains linked by disulfide bonds. HGFA/HGF Activator Protein, Human (HEK293, His) is the recombinant human-derived HGFA/HGF Activator protein, expressed by HEK293 , with C-His labeled tag.

HGFA/HGF Activator is a pivotal enzyme in cellular processes as it catalyzes the activation of hepatocyte growth factor (HGF) by transforming it from a single-chain form into a biologically active heterodimeric configuration. This activation mechanism involves the formation of a heterodimer, consisting of a short chain and a long chain, intricately connected by a disulfide bond. This heterodimeric structure underscores the essential role of HGFA in modulating HGF activity, implicating its significance in various cellular responses and signaling pathways.

Measured by its ability to cleave 10 μM peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 that incubate at room temperature for 30 minutes .The specific activity is 33.56-47.99 pmol/min/µg.

Human

HEK293

C-His

Q04756/NP_001519.1 (Q36-S655)

3083  [NCBI]

Full Length of Mature Protein

QPGGNRTESPEPNATATPAIPTILVTSVTSETPATSAPEAEGPQSGGLPPPPRAVPSSSSPQAQALTEDGRPCRFPFRYGGRMLHACTSEGSAHRKWCATTHNYDRDRAWGYCVEATPPPGGPAALDPCASGPCLNGGSCSNTQDPQSYHCSCPRAFTGKDCGTEKCFDETRYEYLEGGDRWARVRQGHVEQCECFGGRTWCEGTRHTACLSSPCLNGGTCHLIVATGTTVCACPPGFAGRLCNIEPDERCFLGNGTGYRGVASTSASGLSCLAWNSDLLYQELHVDSVGAAALLGLGPHAYCRNPDNDERPWCYVVKDSALSWEYCRLEACESLTRVQLSPDLLATLPEPASPGRQACGRRHKKRTFLRPRIIGGSSSLPGSHPWLAAIYIGDSFCAGSLVHTCWVVSAAHCFSHSPPRDSVSVVLGQHFFNRTTDVTQTFGIEKYIPYTLYSVFNPSDHDLVLIRLKKKGDRCATRSQFVQPICLPEPGSTFPAGHKCQIAGWGHLDENVSGYSSSLREALVPLVADHKCSSPEVYGADISPNMLCAGYFDCKSDACQGDSGGPLACEKNGVAYLYGIISWGDGCGRLHKPGVYTRVANYVDWINDRIRPPRRLVAPS

Approximately 95-110 kDa.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% - 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.