HGFA/HGF Activator Protein, Human (HEK293, His)

The HGFA/HGF Activator Protein serves a crucial role by catalyzing the activation of hepatocyte growth factor (HGF), transforming it into a biologically active heterodimer. This process involves the formation of a heterodimer with a short and long chain connected by a disulfide bond. The resulting heterodimeric structure highlights HGFA's essential role in modulating HGF activity, emphasizing its significance in diverse cellular responses and signaling pathways. HGFA/HGF Activator Protein, Human (HEK293, His) is the recombinant human-derived HGFA/HGF Activator protein, expressed by HEK293, with C-His labeled tag. The total length of HGFA/HGF Activator Protein, Human (HEK293, His) is 655 a.a., with molecular weight of ~34 & 37 & 65 & 105 KDa, respectively.

HGFA/HGF Activator is a pivotal enzyme in cellular processes as it catalyzes the activation of hepatocyte growth factor (HGF) by transforming it from a single-chain form into a biologically active heterodimeric configuration. This activation mechanism involves the formation of a heterodimer, consisting of a short chain and a long chain, intricately connected by a disulfide bond. This heterodimeric structure underscores the essential role of HGFA in modulating HGF activity, implicating its significance in various cellular responses and signaling pathways.

Human

HEK293

C-His

Q04756 (M1-S655)

3083  [NCBI]

Approximately 34&37&65&105 kDa.

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.