HGFA/HGF Activator Protein, Human (HEK293, His)
Based on 1 Customer Validation
HGFA/HGF-activating proteins play a crucial role by catalyzing the activation of hepatocyte growth factor (HGF) and converting it into biologically active heterodimers. The process involves the formation of heterodimers with short and long chains linked by disulfide bonds. HGFA/HGF Activator Protein, Human (HEK293, His) is the recombinant human-derived HGFA/HGF Activator protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
HGFA/HGF-activating proteins play a crucial role by catalyzing the activation of hepatocyte growth factor (HGF) and converting it into biologically active heterodimers. The process involves the formation of heterodimers with short and long chains linked by disulfide bonds. HGFA/HGF Activator Protein, Human (HEK293, His) is the recombinant human-derived HGFA/HGF Activator protein, expressed by HEK293 , with C-His labeled tag.
Background
HGFA/HGF Activator is a pivotal enzyme in cellular processes as it catalyzes the activation of hepatocyte growth factor (HGF) by transforming it from a single-chain form into a biologically active heterodimeric configuration. This activation mechanism involves the formation of a heterodimer, consisting of a short chain and a long chain, intricately connected by a disulfide bond. This heterodimeric structure underscores the essential role of HGFA in modulating HGF activity, implicating its significance in various cellular responses and signaling pathways.
Verified Bioactivity
Measured by its ability to cleave 10 μM peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 that incubate at room temperature for 30 minutes .The specific activity is 33.56-47.99 pmol/min/µg.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
Q04756/NP_001519.1 (Q36-S655)
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Molecular Construction
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N-term
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HGFA (Q36-S655)
Accession # Q04756/NP_001519.1 -
His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
HGFAC; Serine Protease HGFAC; HGF Activator; HGFA Serine Protease; HGFA; HGFAP; Hepatocyte Growth Factor Activator Serine Protease; Hepatocyte Growth Factor Activator
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AA Sequence
QPGGNRTESPEPNATATPAIPTILVTSVTSETPATSAPEAEGPQSGGLPPPPRAVPSSSSPQAQALTEDGRPCRFPFRYGGRMLHACTSEGSAHRKWCATTHNYDRDRAWGYCVEATPPPGGPAALDPCASGPCLNGGSCSNTQDPQSYHCSCPRAFTGKDCGTEKCFDETRYEYLEGGDRWARVRQGHVEQCECFGGRTWCEGTRHTACLSSPCLNGGTCHLIVATGTTVCACPPGFAGRLCNIEPDERCFLGNGTGYRGVASTSASGLSCLAWNSDLLYQELHVDSVGAAALLGLGPHAYCRNPDNDERPWCYVVKDSALSWEYCRLEACESLTRVQLSPDLLATLPEPASPGRQACGRRHKKRTFLRPRIIGGSSSLPGSHPWLAAIYIGDSFCAGSLVHTCWVVSAAHCFSHSPPRDSVSVVLGQHFFNRTTDVTQTFGIEKYIPYTLYSVFNPSDHDLVLIRLKKKGDRCATRSQFVQPICLPEPGSTFPAGHKCQIAGWGHLDENVSGYSSSLREALVPLVADHKCSSPEVYGADISPNMLCAGYFDCKSDACQGDSGGPLACEKNGVAYLYGIISWGDGCGRLHKPGVYTRVANYVDWINDRIRPPRRLVAPS
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Molecular Weight
Approximately 34, 37, 65, 105 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% - 8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (239 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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