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  3. HLA-A*0301 KRAS G12V Complex Protein, Human (HEK293, His-Avi)

HLA-A*0301 KRAS G12V Complex Protein, Human (HEK293, His-Avi)

Cat. No.: HY-P77781
Handling Instructions

HLA-A*0301 collaborates with B2M to present viral and tumor peptides, guiding CD8-positive T cell responses against infected or transformed cells. It can also present self-peptides, avoiding autoreactivity. The antigen recognition specificity involves the peptide and MHC residues, with HLA-A*0301 presenting 8 to 13 amino acid peptides, characterized by allele-specific binding motifs. Specific alleles, like A*01:01 and A*03:01, present a distinct repertoire of peptides, including viral and tumor epitopes. HLA-A*0301 KRAS G12V Complex Protein, Human (HEK293, His-Avi) is a recombinant protein dimer complex containing HLA-A*0301 and B2M/Beta-2-microglobulin Protein, expressed by HEK293 , with C-Avi, C-His labeled tag and VVGAVGVGK peptide. HLA-A*0301 KRAS G12V Complex Protein, Human (HEK293, His-Avi), has molecular weight of 55-60 kDa.

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Description

HLA-A*0301 collaborates with B2M to present viral and tumor peptides, guiding CD8-positive T cell responses against infected or transformed cells. It can also present self-peptides, avoiding autoreactivity. The antigen recognition specificity involves the peptide and MHC residues, with HLA-A*0301 presenting 8 to 13 amino acid peptides, characterized by allele-specific binding motifs. Specific alleles, like A*01:01 and A*03:01, present a distinct repertoire of peptides, including viral and tumor epitopes. HLA-A*0301 KRAS G12V Complex Protein, Human (HEK293, His-Avi) is a recombinant protein dimer complex containing HLA-A*0301 and B2M/Beta-2-microglobulin Protein, expressed by HEK293 , with C-Avi, C-His labeled tag and VVGAVGVGK peptide. HLA-A*0301 KRAS G12V Complex Protein, Human (HEK293, His-Avi), has molecular weight of 55-60 kDa.

Background

HLA-A*0301, a critical antigen-presenting major histocompatibility complex class I (MHCI) molecule, collaborates with B2M/beta 2 microglobulin to predominantly display viral and tumor-derived peptides on antigen-presenting cells. This presentation allows recognition by alpha-beta T cell receptor (TCR) on HLA-A-restricted CD8-positive T cells, thereby guiding antigen-specific T cell immune responses to eliminate infected or transformed cells. Additionally, HLA-A*0301 may present self-peptides derived from the signal sequence of secreted or membrane proteins, although T cells specific for these peptides are typically inactivated to prevent autoreactivity. The recognition of both the peptide and the MHC molecule by TCR underscores the fine specificity of antigen recognition, with the peptide responsible for this specificity and MHC residues accounting for the MHC restriction of T cells. HLA-A*0301 typically presents intracellular peptide antigens of 8 to 13 amino acids resulting from cytosolic proteolysis via IFNG-induced immunoproteasome or via endopeptidase IDE/insulin-degrading enzyme. It can bind different peptides containing allele-specific binding motifs primarily defined by anchor residues at positions 2 and 9. Notably, specific alleles, such as A*01:01 and A*03:01, present a restricted repertoire of peptides, including viral and tumor epitopes, each characterized by distinct motifs and anchors.

Species

Human

Source

HEK293

Tag

C-Avi;C-His

Accession

NP_002107.3 (G25-T305)&P61769 (I21-M119)&VVGAVGVGK

Gene ID

3105  [NCBI]&567  [NCBI]

Synonyms
MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD
Molecular Weight

55-60 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

HLA-A*0301 KRAS G12V Complex Protein, Human (HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
HLA-A*0301 KRAS G12V Complex Protein, Human (HEK293, His-Avi)
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HY-P77781
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