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HLA-A*1101 KRAS G12V Complex Protein, Human (HEK293, His-Avi)

Cat. No.: HY-P77786
COA Handling Instructions

HLA-A*1101as is a key MHCI molecule that presents viral and tumor-derived peptides that direct T cell immune responses against infected or transformed cells. It collaborates with B2M to showcase a diverse peptide library. HLA-A*1101 KRAS G12V Complex Protein, Human (HEK293, His-Avi) is a recombinant protein dimer complex containing HLA-A*1101 and B2M/Beta-2-microglobulin Protein, expressed by HEK293 , with C-Avi, C-His labeled tag and VVGAVGVGK peptide. HLA-A*1101 KRAS G12V Complex Protein, Human (HEK293, His-Avi), has molecular weight of 42-46 kDa.

For research use only. We do not sell to patients.

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10 μg $175 In-stock
50 μg $490 In-stock
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Description

HLA-A*1101as is a key MHCI molecule that presents viral and tumor-derived peptides that direct T cell immune responses against infected or transformed cells. It collaborates with B2M to showcase a diverse peptide library. HLA-A*1101 KRAS G12V Complex Protein, Human (HEK293, His-Avi) is a recombinant protein dimer complex containing HLA-A*1101 and B2M/Beta-2-microglobulin Protein, expressed by HEK293 , with C-Avi, C-His labeled tag and VVGAVGVGK peptide. HLA-A*1101 KRAS G12V Complex Protein, Human (HEK293, His-Avi), has molecular weight of 42-46 kDa.

Background

HLA-A*1101, an antigen-presenting major histocompatibility complex class I (MHCI) molecule, serves a pivotal role in immune responses by presenting predominantly viral and tumor-derived peptides on antigen-presenting cells. This presentation facilitates the recognition of these peptides by the alpha-beta T cell receptor (TCR) on HLA-A-restricted CD8-positive T cells, guiding antigen-specific T cell immune responses aimed at eliminating infected or transformed cells. In collaboration with B2M/beta 2 microglobulin, HLA-A*1101 displays a diverse peptide repertoire, encompassing viral epitopes and tumor-associated antigens. Both the presented peptide and the MHCI molecule contribute to the specificity of antigen recognition, with the peptide determining fine specificity and MHCI residues influencing the MHC restriction of T cells. HLA-A*1101 typically presents intracellular peptide antigens of 8 to 13 amino acids arising from cytosolic proteolysis. It can bind different peptides containing allele-specific binding motifs, primarily defined by anchor residues at positions 2 and 9. Allele-specific motifs characterize distinct peptide repertoires, such as A*01:01, presenting viral epitopes and tumor antigens with a common canonical motif. Notably, HLA-A*1101 fails to present highly immunogenic peptides from EBV latent antigens. For A*11:01, it plays a crucial role in controlling infections, presenting immunodominant epitopes from HIV-1, EBV, and HBV, along with contributing to the immune response against SARS-CoV-2.

Species

Human

Source

HEK293

Tag

C-Avi;C-His

Accession

AAV53343 (G25-T305)&P61769 (I21-M119)&VVGAVGVGK

Gene ID

3105  [NCBI]&567  [NCBI]

Synonyms
MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD
Molecular Weight

42-46 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

HLA-A*1101 KRAS G12V Complex Protein, Human (HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
HLA-A*1101 KRAS G12V Complex Protein, Human (HEK293, His-Avi)
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HY-P77786
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