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ICAM-1/CD54 Protein, Rat (HEK293, His)

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ICAM-1/CD54 protein is a crucial ligand for leukocyte adhesion, promoting endothelial apical cup assembly during migration.It regulates immune responses and inflammation.ICAM-1 also interacts with MUC1, promoting cell aggregation, and contributes to immunological synapse formation between antigen-presenting cells and T cells.These interactions showcase ICAM-1's diverse functions in cellular adhesion and immune modulation.ICAM-1/CD54 Protein, Rat (HEK293, His) is the recombinant rat-derived ICAM-1/CD54 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

ICAM-1/CD54 protein is a crucial ligand for leukocyte adhesion, promoting endothelial apical cup assembly during migration.It regulates immune responses and inflammation.ICAM-1 also interacts with MUC1, promoting cell aggregation, and contributes to immunological synapse formation between antigen-presenting cells and T cells.These interactions showcase ICAM-1's diverse functions in cellular adhesion and immune modulation.ICAM-1/CD54 Protein, Rat (HEK293, His) is the recombinant rat-derived ICAM-1/CD54 protein, expressed by HEK293 , with C-His labeled tag.

Background

ICAM-1/CD54 protein serves as a pivotal ligand for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2), playing a critical role in diverse cellular interactions. Specifically, during leukocyte trans-endothelial migration, engagement of ICAM-1 promotes the assembly of endothelial apical cups through the activation of ARHGEF26/SGEF and RHOG. This intricate molecular process, reminiscent of other ICAM family members, underscores ICAM-1's involvement in regulating immune responses and inflammatory reactions. Beyond its role in leukocyte adhesion, ICAM-1 forms homodimers and interacts with MUC1, facilitating cell aggregation in epithelial cells. Furthermore, ICAM-1 engages with ARHGEF26/SGEF and interacts on the T cell side with CD81, CD247, and CD9, contributing to the establishment of immunological synapses between antigen-presenting cells and T cells. These versatile interactions highlight the multifaceted functions of ICAM-1 in cellular adhesion and immune modulation.

Verified Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to rat ICAM1 coated plates (12.5 µg/mL, 100 µL/well), approximately > 30% cells will adhere specifically.

Technical Parameters

  • Species Rat
  • Source HEK293
  • Tag C-His
  • Accession

    Q00238 (Q28-T493)

  • Gene ID
    25464  [NCBI]
  • Molecular Construction
    • N-term
    • ICAM-1 (Q28-T493)
      Accession # Q00238
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    ICAM1; Epididymis Secretory Sperm Binding Protein; Intercellular Adhesion Molecule 1; Intercellular Adhesion Molecule-1; CD54; Cell Surface Glycoprotein P3.58; BB2; Human Rhinovirus Receptor; Major Group Rhinovirus Receptor; CD54 Antigen; ICAM-1; P3.58; I

  • AA Sequence

    QVSIHPTEAFLPRGGSVQVNCSSSCEDENLGLGLETNWMKDELSSGHNWKLFKLSDIGEDSRPLCFENCGTTQSSASATITVYSFPERVELDPLPAWQQVGKNLILRCLVEGGAPRTQLSVVLLRGNETLSRQAVDGDPKEITFTVLASRGDHGANFSCFTELDLRPQGLSLFKNVSEVRQLRTFDLPTRVLKLDTPDLLEVGTQQKFLCSLEGLFPASEAQIYLEMGGQMLTLESTNSRDFVSATASVEVTEKLDRTLQLRCVLELADQTLEMEKTLRIYNFSAPILTLSQPEVSEGDQVTVKCEAHGGAQVVLLNSTSPRPPTSQGTSPRPPTSQIQFTLNASPEDHKRRFFCSAALEVDGKSLFKNQTLELHVLYGPHLDKKDCLGNWTWQEGSQQTLTCQPQGNPAPNLTCSRKADGVPLPIGMVKSVKREMNGTYKCRAFSSRGSITRDVHLTVLYHDQNT

  • Predicted Molecular Mass

    52.7 kDa

  • Molecular Weight

    Approximately 70-75 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
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Biological Activity (ED50) Biological Activity (ED50)
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MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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