IFN-alpha 1/IFNA1 Protein, Rat (HEK293, His, solution)
Based on 1 Customer Validation
IFN-alpha 1/IFNA1 Protein, Rat (HEK293, His, solution) is the recombinant rat-derived IFN-alpha 1/IFNA1, expressed by HEK293 , with C-His labeled tag.
- Species: Rat
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Biological Activity
Description
IFN-alpha 1/IFNA1 Protein, Rat (HEK293, His, solution) is the recombinant rat-derived IFN-alpha 1/IFNA1, expressed by HEK293 , with C-His labeled tag.
Verified Bioactivity
Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV).The ED50 for this effect is typically 4-20 ng/mL.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Rat
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Source HEK293
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Tag C-His
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Accession
P05011 (C24-S192)
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Molecular Construction
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N-term
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IFNA1 (C24-S192)
Accession # P05011 -
His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
IFNA1; Interferon Alpha-1; Interferon Alpha 1; Interferon Alpha-D; IFN-AlphaD; Interferon-Alpha1; IFN-ALPHA; IFN-Alpha-1; IFNA13; LeIF D; IFNA@; Interferon Alpha-1/13; IFL; IFN-Alpha-1/13; IFN; IFN-Alpha 1b; Interferon Alpha 1b
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AA Sequence
CDLPHTHNLRNKRVFTLLAQMRRLSPVSCLKDRKYFGFPLEKVDGQQIQKAQAIPVLHELTQQILSLFTSKESSTAWDATLLDSFCNDLQQQLSGLQACLMQQVGVQESPLTQEDSLLAVREYFHRITVYLRENKHSPCAWEVVKAEVWRALSSSANLMGRLREERNES
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Predicted Molecular Mass
20.9 kDa
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Molecular Weight
Approximately 20-25 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (233 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)